Factors affecting the autologous mixed lymphocyte reaction in kidney transplantation
Ημερομηνία
1983Συγγραφέας
Fuller, L.Flaa, C.
Jaffe, D.
Strauss, J.
Kyriakides, George K.
Miller, Jody C.
Source
Journal of Clinical InvestigationVolume
71Issue
5Pages
1322-1330Google Scholar check
Metadata
Εμφάνιση πλήρους εγγραφήςΕπιτομή
In long-term well adapted kidney transplant recipients we have found a close correlation between the T helper (T(H)):T suppressor/cytotoxic (T(S/C)) subset ratios and the presence of T cells that respond in the autologous mixed lymphocyte reaction (AMLR). In 21 recipients with T cell E rosette levels ranging between 53 and 86% and T(H):T(S/C) ratios between 0.15 to 2.10, ratios of > 0.8 correlated with AMLR responses (13/13), and ratios of < 0.8 with AMLR nonreactivity (7/7). By contrast, the allogeneic MLR showed no apparent correlation with the T(H):T(S/C) ratios or with the AMLR pre- or postoperatively. It was found that the AMLR in 22 of 23 normal individuals was markedly inhibited by autologous T cells obtained from peripheral blood lymphocytes, exposed to 3,000 rad (Tx) and added as a third component to the cultures. In contrast, 13 of 13 kidney transplant recipients failed to exhibit this Tx AMLR inhibitory cell population. The 'naturally occurring' T inhibitory cells, fractionated by an affinity column chromatography procedure into x-irradiated T(H) and T(S/C) subsets, inhibited the AMLR to the same extent as unseparated Tx cells. In cell interchange studies performed in four of five HLA identical donor-recipient AMLR (immunosuppressed), but recipient Tx cells failed to inhibit the donor AMLR. Finally T cells, primed in AMLR and allogeneic MLR for 10 d were tested for AMLR or allogeneic MLR inhibitory activity. Allogeneic MLR primed x-irradiated cells, inhibited both the AMLR and allogeneic MLR while AMLR x-irradiated primed cells inhibited neither reaction. The Tx AMLR inhibitor found in normal peripheral blood, appears to be a cell that is highly sensitive to the effects of biologic or pharmacologic immunosuppressive agents.