A Prototype Device for 3D Regional, Passive Elasticity Measurements of the Murine Myocardium Using AFM
Date
2016Author
Constantinides, C.Kossivas, F.
Epameinonda, P.
Michaelides, M.
Rebholz, Claus
Source
Experimental TechniquesVolume
40Pages
43-48Google Scholar check
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This study presents the design and implementation of a prototype device that interfaces with commercial atomic force microscopy (AFM) instruments to accurately measure ex vivo, regional, passive elasticity measurements of the murine myocardium in three-dimensions (3D). The constructed prototype has a translation stage base allows fine adjustment of planar location at the micrometer level of accuracy. Four degrees of motional freedom are allowed in total, permitting fine, independent rotations of the murine heart (oriented at right-angles with respect to its normal mode of operation) at 2-5° increments, along its two axes of symmetry. Successful mounting of the constructed prototype was realized on the AFM's base platform, with Zpositioning maintained through the instrument's electronics. Measurements of normal epicardial tissue elasticity in one excised male C57BL/6 heart led to a mean value (over 15 epicardial locations) of 51.8 ± 5.4 kPa. A flexible, versatile, and low-cost prototype assembly was designed, implemented, and tested to conduct epicardial elasticity measurements in a semi-automatedmanner, using AFM. The epicardial elasticity values are within expected, published ranges for the normal myocardium of 10-55 kPa. Efforts are ongoing to allow 3D regional elasticity measurements at finer resolution with potential usefulness to studies of genetically altered mice. ABSTRACT FROM AUTHOR] Copyright of Experimental Techniques is the property of Springer Science & Business Media B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)