dc.contributor.author | Kirmizis, Antonis | en |
dc.contributor.author | Bartley, S. M. | en |
dc.contributor.author | Kuzmichev, A. | en |
dc.contributor.author | Margueron, R. | en |
dc.contributor.author | Reinberg, D. | en |
dc.contributor.author | Green, R. | en |
dc.contributor.author | Farnham, P. J. | en |
dc.creator | Kirmizis, Antonis | en |
dc.creator | Bartley, S. M. | en |
dc.creator | Kuzmichev, A. | en |
dc.creator | Margueron, R. | en |
dc.creator | Reinberg, D. | en |
dc.creator | Green, R. | en |
dc.creator | Farnham, P. J. | en |
dc.date.accessioned | 2019-11-04T12:51:50Z | |
dc.date.available | 2019-11-04T12:51:50Z | |
dc.date.issued | 2004 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53172 | |
dc.description.abstract | Polycomb group (PcG) complexes 2 and 3 are involved in transcriptional silencing. These complexes contain a histone lysine methyltransferase (HKMT) activity that targets different lysine residues on histones H1 or H3 in vitro. However, it is not known if these histones are methylation targets in vivo because the human PRC2/3 complexes have not been studied in the context of a natural promoter because of the lack of known target genes. Here we report the use of RNA expression arrays and CpG-island DNA arrays to identify and characterize human PRC2/3 target genes. Using oligonucleotide arrays, we first identified a cohort of genes whose expression changes upon siRNA-mediated removal of Suz12, a core component of PRC2/3, from colon cancer cells. To determine which of the putative target genes are directly bound by Suz12 and to precisely map the binding of Suz12 to those promoters, we combined a high-resolution chromatin immunoprecipitation (ChIP) analysis with custom oligonucleotide promoter arrays. We next identified additional putative Suz12 target genes by using ChIP coupled to CpG-island microarrays. We showed that HKMT-Ezh2 and Eed, two other components of the PRC2/3 complexes, colocalize to the target promoters with Suz12. Importantly, recruitment of Suz12, Ezh2 and Eed to target promoters coincides with methylation of histone H3 on Lys 27. | en |
dc.source | Genes and Development | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-3042781030&doi=10.1101%2fgad.1200204&partnerID=40&md5=64a7c17ea774d706881fb5e07803c65b | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | human tissue | en |
dc.subject | promoter region | en |
dc.subject | Gene Expression Regulation | en |
dc.subject | unclassified drug | en |
dc.subject | regulator protein | en |
dc.subject | human cell | en |
dc.subject | RNA | en |
dc.subject | small interfering RNA | en |
dc.subject | transcription regulation | en |
dc.subject | DNA | en |
dc.subject | gene targeting | en |
dc.subject | DNA microarray | en |
dc.subject | Transcription Factors | en |
dc.subject | DNA-Binding Proteins | en |
dc.subject | Colonic Neoplasms | en |
dc.subject | gene silencing | en |
dc.subject | cancer cell culture | en |
dc.subject | RNA analysis | en |
dc.subject | protein localization | en |
dc.subject | Promoter Regions (Genetics) | en |
dc.subject | immunoprecipitation | en |
dc.subject | Proteins | en |
dc.subject | repressor protein | en |
dc.subject | Repressor Proteins | en |
dc.subject | Carrier Proteins | en |
dc.subject | histone H3 | en |
dc.subject | Histone methylation | en |
dc.subject | Histones | en |
dc.subject | lysine | en |
dc.subject | RNA interference | en |
dc.subject | Chromatin immunoprecipitation | en |
dc.subject | CpG island | en |
dc.subject | CpG Islands | en |
dc.subject | Eed | en |
dc.subject | embryonic ectoderm development protein | en |
dc.subject | histone lysine methyltransferase | en |
dc.subject | Histone-Lysine N-Methyltransferase | en |
dc.subject | Macromolecular Substances | en |
dc.subject | Methylation | en |
dc.subject | Oligonucleotide Array Sequence Analysis | en |
dc.subject | Polycomb | en |
dc.subject | polycomb group protein | en |
dc.subject | polycomb repressive complex 2 protein | en |
dc.subject | polycomb repressive complex 3 protein | en |
dc.subject | protein ezh2 | en |
dc.subject | protein methylation | en |
dc.subject | protein suz12 | en |
dc.subject | Suz12 | en |
dc.title | Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27 | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1101/gad.1200204 | |
dc.description.volume | 18 | |
dc.description.startingpage | 1592 | |
dc.description.endingpage | 1605 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :364</p> | en |
dc.source.abbreviation | Genes Dev. | en |
dc.contributor.orcid | Kirmizis, Antonis [0000-0002-3748-8711] | |
dc.gnosis.orcid | 0000-0002-3748-8711 | |