Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy
Ημερομηνία
1999Συγγραφέας
Lewin, S. R.Vesanen, M.
Kostrikis, Leontios G.
Hurley, A.
Duran, M.
Zhang, L.
Ho, David D.
Markowitz, M.
Gibson, U. E.
Heid, C. A.
ISSN
0022-538XSource
Journal of virologyVolume
73Pages
6099-6103Google Scholar check
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Metadata
Εμφάνιση πλήρους εγγραφήςΕπιτομή
We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.
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