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Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy

dc.contributor.authorLewin, S. R.en
dc.contributor.authorVesanen, M.en
dc.contributor.authorKostrikis, Leontios G.en
dc.contributor.authorHurley, A.en
dc.contributor.authorDuran, M.en
dc.contributor.authorZhang, L.en
dc.contributor.authorHo, David D.en
dc.contributor.authorMarkowitz, M.en
dc.contributor.authorGibson, U. E.en
dc.contributor.authorHeid, C. A.en
dc.creatorLewin, S. R.en
dc.creatorVesanen, M.en
dc.creatorKostrikis, Leontios G.en
dc.creatorHurley, A.en
dc.creatorDuran, M.en
dc.creatorZhang, L.en
dc.creatorHo, David D.en
dc.creatorMarkowitz, M.en
dc.creatorGibson, U. E.en
dc.creatorHeid, C. A.en
dc.date.accessioned2019-11-04T12:52:17Z
dc.date.available2019-11-04T12:52:17Z
dc.date.issued1999
dc.identifier.issn0022-538X
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53226
dc.description.abstractWe have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individualsen
dc.description.abstracthowever, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.en
dc.sourceJournal of virologyen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0033031005&partnerID=40&md5=f50d0343454db92ea51ecf897e7d4067
dc.subjectarticleen
dc.subjecthumanen
dc.subjectHumansen
dc.subjectadulten
dc.subjectpriority journalen
dc.subjectclinical articleen
dc.subjectTime Factorsen
dc.subjectlamivudineen
dc.subjectzidovudineen
dc.subjectHIV Infectionsen
dc.subjectnonhumanen
dc.subjectimmunosuppressive treatmenten
dc.subjectviremiaen
dc.subjectvirus loaden
dc.subjectvirus RNAen
dc.subjectpolymerase chain reactionen
dc.subjectmessenger RNAen
dc.subjectLongitudinal Studiesen
dc.subjectmononuclear cellen
dc.subjectAnti-HIV Agentsen
dc.subjectindinaviren
dc.subjectHIV-1en
dc.subjectvirus replicationen
dc.subjectantivirus agenten
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjecthuman immunodeficiency virus infectionen
dc.subjectritonaviren
dc.subjectsaquinaviren
dc.subjectRNA, Messengeren
dc.subjectRNA, Viralen
dc.subjectvirus isolationen
dc.subjectLeukocytes, Mononuclearen
dc.subjectCD4 Lymphocyte Counten
dc.subjecthuman immunodeficiency virus 1en
dc.subjectvirus detectionen
dc.subjectvirus expressionen
dc.titleUse of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapyen
dc.typeinfo:eu-repo/semantics/article
dc.description.volume73
dc.description.startingpage6099
dc.description.endingpage6103
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :183</p>en
dc.source.abbreviationJ.Virol.en
dc.contributor.orcidKostrikis, Leontios G. [0000-0002-5340-7109]
dc.gnosis.orcid0000-0002-5340-7109


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