Genistein induces apoptosis and topoisomerase II-mediated DNA breakage in colon cancer cells
Ημερομηνία
2000Συγγραφέας
Salti, G. I.Grewal, S.
Mehta, R. R.
Das Gupta, T. K.
Boddie, A. W., Jr.
Constantinou, Andreas I.
Source
European journal of cancerVolume
36Pages
796-802Google Scholar check
Keyword(s):
Metadata
Εμφάνιση πλήρους εγγραφήςΕπιτομή
The present study was undertaken to determine if (a) genistein induces topo II-mediated DNA damage in HT-29 colon cancer cells and (b) if this damage is required to induce apoptosis. DNA damage was evaluated using the comet assay. Apoptosis was determined by the ethidium bromide/acridine orange staining technique. DNA breakage was noted within 1 h of treatment. Apoptosis was only induced with high concentrations (≥60 μM) of genistein. Marked inhibition of HT-29 cell growth was evident at concentrations ranging from 60 to 150 μM. This was associated with a cell cycle arrest at G2/M. Similar findings were obtained in SW-620 and SW-1116 colon cancer cell lines. Aclarubicin, a topo II antagonist, reduced genistein-induced DNA breaks but did not reduce apoptosis. These data suggest that, in colon cancer cells, topo II serves as the enzymatic target of genistein. Furthermore, topo II-mediated DNA cleavage is not required for the induction of apoptosis. © 2000.