Browsing by Author "Skourides, Paris A."
Now showing items 21-27 of 27
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Article
Samba, a Xenopus hnRNP expressed in neural and neural crest tissues
Yan, C. Y. I.; Skourides, Paris A.; Chang, Christopher C.; Brivanlou, A. (2009)RNA binding proteins regulate gene expression at the posttranscriptional level and play important roles in embryonic development. Here, we report the cloning and expression of Samba, a Xenopus hnRNP that is maternally ...
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Article
A simple mechanochemical model for calcium signalling in embryonic epithelial cells
Kaouri, K.; Maini, P. K.; Skourides, Paris A.; Christodoulou, N.; Chapman, S. J. (2019)Calcium signalling is one of the most important mechanisms of information propagation in the body. In embryogenesis the interplay between calcium signalling and mechanical forces is critical to the healthy development of ...
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Book Chapter
Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus
Ioannou, Androulla; Eleftheriou, Iro; Charalambous, Anna; Skourides, Paris A. (Springer New York, 2015)The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount ...
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Article
Single-shot optical sectioning using two-color probes in HiLo fluorescence microscopy
Muro, E.; Vermeulen, P.; Ioannou, Androulla; Skourides, Paris A.; Dubertret, B.; Fragola, A.; Loriette, V. (2011)We describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving ...
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Article
Spatially and temporally regulated α6 integrin cleavage during Xenopus laevis development
Demetriou, Manolis C.; Stylianou, Panayiota; Andreou, Maria I.; Yiannikouri, Olga; Tsaprailis, George; Cress, Anne E.; Skourides, Paris A. (2008)The α6 integrin is essential for early nervous system development in Xenopus laevis. We have previously reported a uPA cleaved form of integrin α6 (α6p), in invasive human prostate cancer tissue, whose presence correlates ...
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Article
Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2011)Background: Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within ...
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Article
Xenopus laevis nucleotide binding protein 1 (xNubp1) is important for convergent extension movements and controls ciliogenesis via regulation of the actin cytoskeleton
Ioannou, Androulla; Santama, Niovi; Skourides, Paris A. (2013)Nucleotide binding protein 1 (Nubp1) is a highly conserved phosphate loop (P-loop) ATPase involved in diverse processes including iron-sulfur protein assembly, centrosome duplication and lung development. Here, we report ...