Browsing by Author "Daskalakis, Vangelis"
Now showing items 1-8 of 8
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Coupling of helix E-F motion with the O-nitrito and 2-nitrovinyl coordination in myoglobin
Ioannou, Androulla; Lambrou, Alexandra; Daskalakis, Vangelis; Pinakoulaki, Eftychia (2017)Myoglobin (Mb) is known to react slowly with nitirite to form the green pigment by NO2 − cordination to the heme Fe in the O-binding nitrito (O1[sbnd]N[dbnd]O2) mode and to the heme 2-vinyl position. Nitrite is a powerful ...
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Article
Nitrite coordination in myoglobin
Ioannou, Androulla; Lambrou, Alexandra; Daskalakis, Vangelis; Pinakoulaki, Eftychia (2017)The coordination of nitrite in myoglobin (Mb) has been characterized by resonance Raman spectroscopy and the frequencies of the nitrite bound to the heme Fe as well to the 2-vinyl have been computed by density functional ...
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The origin of the Fe IV = O intermediates in cytochrome aa 3 oxidase
Pinakoulaki, Eftychia; Daskalakis, Vangelis; Varotsis, Constantinos (2012)The dioxygen reduction mechanism in cytochrome oxidases relies on proton control of the electron transfer events that drive the process. Proton delivery and proton channels in the protein that are relevant to substrate ...
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Probing the environment of CuB in heme-copper oxidases
Daskalakis, Vangelis; Pinakoulaki, Eftychia; Stavrakis, Stavros; Varotsis, Constantinos (2007)Time-resolved step-scan FTIR (TRS2-FTIR) and density functional theory have been applied to probe the structural dynamics of CuB in heme-copper oxidases at room temperature. The TRS2-FTIR data of cbb3 from Pseudomonas ...
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The protein effect in the structure of two ferryl-oxo intermediates at the same oxidation level in the heme copper binuclear center of cytochrome c oxidase
Pinakoulaki, Eftychia; Daskalakis, Vangelis; Ohta, T.; Richter, O. -M H.; Budiman, K.; Kitagawa, T.; Ludwig, B.; Varotsis, Constantinos (2013)Identification of the intermediates and determination of their structures in the reduction of dioxygen to water by cytochrome c oxidase (CcO) are particularly important to understanding both O2 activation and proton pumping ...
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Structural dynamics of heme-copper oxidases and nitric oxide reductases: Time-resolved step-scan Fourier transform infrared and time-resolved resonance Raman studies
Pinakoulaki, Eftychia; Koutsoupakis, Constantinos; Stavrakis, Stavros; Aggelaki, Marialena; Papadopoulos, George Angelos; Daskalakis, Vangelis; Varotsis, Constantinos (2005)Of the spectroscopic methods available for the characterization of the dynamics of heme protein active sites, time-resolved resonance Raman spectroscopy (TR3) is a powerful technique because excitation within the heme π-π* ...
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Time-resolved step-scan Fourier transform infrared investigation of heme-copper oxidases: Implications for O2 input and H 2O/H+ output channels
Koutsoupakis, Constantinos; Pinakoulaki, Eftychia; Stavrakis, Stavros; Daskalakis, Vangelis; Varotsis, Constantinos (2004)We have applied FTIR and time-resolved step-scan Fourier transform infrared (TRS2-FTIR) spectroscopy to investigate the dynamics of the heme-CuB binuclear center and the protein dynamics of mammalian aa3, Pseudomonas ...
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Two ligand-binding sites in the O2-sensing signal transducer HemAT: Implications for ligand recognition/discrimination and signaling
Pinakoulaki, Eftychia; Yoshimura, H.; Daskalakis, Vangelis; Yoshioka, S.; Aono, S.; Varotsis, Constantinos (2006)We have identified a ligand (CO) accommodation cavity in the signal transducer sensor protein HemAT (heme-based aerotactic transducer) that allows us to gain single-molecule insights into the mechanism of gas sensor proteins. ...