Browsing by Author "Ioannou, Androulla"
Now showing items 1-12 of 12
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Article
Comparing intracellular stability and targeting of sulfobetaine quantum dots with other surface chemistries in live cells
Muro, E.; Fragola, A.; Pons, T.; Lequeux, N.; Ioannou, Androulla; Skourides, Paris A.; Dubertret, B. (2012)The in vivo labeling of intracellular components with quantum dots (QDs) is very limited because of QD aggregation in the cell cytoplasm and/or QD confinement into lysosomal compartments. In order to improve intracellular ...
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Article
Coupling of helix E-F motion with the O-nitrito and 2-nitrovinyl coordination in myoglobin
Ioannou, Androulla; Lambrou, Alexandra; Daskalakis, Vangelis; Pinakoulaki, Eftychia (2017)Myoglobin (Mb) is known to react slowly with nitirite to form the green pigment by NO2 − cordination to the heme Fe in the O-binding nitrito (O1[sbnd]N[dbnd]O2) mode and to the heme 2-vinyl position. Nitrite is a powerful ...
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Article
High-resolution whole-mount in situ hybridization using quantum dot nanocrystals
Ioannou, Androulla; Eleftheriou, Iro; Lubatti, Andrea; Charalambous, Anna; Skourides, Paris A. (2012)The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically ...
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Article
Nitrite coordination in myoglobin
Ioannou, Androulla; Lambrou, Alexandra; Daskalakis, Vangelis; Pinakoulaki, Eftychia (2017)The coordination of nitrite in myoglobin (Mb) has been characterized by resonance Raman spectroscopy and the frequencies of the nitrite bound to the heme Fe as well to the 2-vinyl have been computed by density functional ...
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Article
Probing nitrite coordination in horseradish peroxidase by resonance Raman spectroscopy: Detection of two binding sites
Ioannou, Androulla; Pinakoulaki, Eftychia (2017)Nitrite is a powerful oxidant that affects the activity of peroxidases towards various substrates and leads to heme macrocycle modifications in members of the peroxidase family, such as the horseradish peroxidase (HRP). ...
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Article
Resonance Raman detection of the heme Fe(II)-NO/2-nitrovinyl species in myoglobin
Ioannou, Androulla; Pinakoulaki, Eftychia (2018)The six-coordinate heme Fe(II)-NO/2-nitrovinyl species in myoglobin has been detected and characterized by resonance Raman spectroscopy. The Fe(II)-14NO and 15N-O stretching frequencies of the ferrous heme nitrosyl/2-nitrovinyl ...
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Book Chapter
Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus
Ioannou, Androulla; Eleftheriou, Iro; Charalambous, Anna; Skourides, Paris A. (Springer New York, 2015)The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount ...
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Article
Single-shot optical sectioning using two-color probes in HiLo fluorescence microscopy
Muro, E.; Vermeulen, P.; Ioannou, Androulla; Skourides, Paris A.; Dubertret, B.; Fragola, A.; Loriette, V. (2011)We describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving ...
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Article
Spectral density ratio based clustering methods for the binary segmentation of protein sequences: A comparative study
Ioannou, Androulla; Fokianos, Konstantinos; Promponas, Vasilis J. (2010)We compare several spectral domain based clustering methods for partitioning protein sequence data. The main instrument for this exercise is the spectral density ratio model, which specifies that the logarithmic ratio of ...
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Article
Spin Crossover in Nitrito-Myoglobin as Revealed by Resonance Raman Spectroscopy
Lambrou, Alexandra; Ioannou, Androulla; Pinakoulaki, Eftychia (2016)The myoglobin (Mb) heme Fe-O-N=O and heme Fe-O-N=O/2-nitrovinyl species have been characterized by resonance Raman spectroscopy. In the heme Fe-O-N=O species, the bound nitrite ligand is removed by solvent exchange, thus ...
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Article
Spin Crossover in Nitrito‐Myoglobin as Revealed by Resonance Raman Spectroscopy
Lambrou, Alexandra; Ioannou, Androulla; Pinakoulaki, Eftychia (2016)
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Article
Xenopus laevis nucleotide binding protein 1 (xNubp1) is important for convergent extension movements and controls ciliogenesis via regulation of the actin cytoskeleton
Ioannou, Androulla; Santama, Niovi; Skourides, Paris A. (2013)Nucleotide binding protein 1 (Nubp1) is a highly conserved phosphate loop (P-loop) ATPase involved in diverse processes including iron-sulfur protein assembly, centrosome duplication and lung development. Here, we report ...