Browsing by Author "Stavrakis, Stavros"
Now showing items 1-7 of 7
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Decay of the transient CuB-CO complex is accompanied by formation of the heme Fe-CO complex of cytochrome cbb3-CO at ambient temperature: Evidence from time-resolved fourier transform infrared spectroscopy
Stavrakis, Stavros; Koutsoupakis, Constantinos; Pinakoulaki, Eftychia; Urban, A.; Saraste, M.; Varotsis, Constantinos (2002)Time-resolved step-scan Fourier infrared spectroscopy has been used to study the CO-bound cbb3-type cytochrome c oxidase from Pseudomonas stutzeri at room temperature. We observe a single band in the FTIR spectrum at 1956 ...
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Fourier transform infrared evidence for a ferric six-coordinate nitrosylheme b3 complex of cytochrome cbb3 oxidase from Pseudomonas stutzeri at ambient temperature
Stavrakis, Stavros; Pinakoulaki, Eftychia; Urbani, A.; Varotsis, Constantinos (2002)We report the first vibrational study of NO bound to an oxidized heme-copper oxidase. Cytochrome cbb3 oxidase from P. stutzeri reduces both O2 and NO to H2O and N2O, respectively. The ferric nitrosyl complex of cbb3 exhibits ...
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Observation of the equilibrium CuB-CO complex and functional implications of the transient heme a3 propionates in cytochrome ba3-CO from Thermus thermophilus. Fourier transform infrared (FTIR) and time-resolved step-scan FTIR studies
Koutsoupakis, Constantinos; Stavrakis, Stavros; Pinakoulaki, Eftychia; Soulimane, T.; Varotsis, Constantinos (2002)We report the first evidence for the existence of the equilibrium CuB 1+-CO species of CO-bound reduced cytochrome ba3 from Thermus thermophilus at room temperature. The frequency of the C-O stretching mode of CuB 1+-CO ...
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Probing the environment of CuB in heme-copper oxidases
Daskalakis, Vangelis; Pinakoulaki, Eftychia; Stavrakis, Stavros; Varotsis, Constantinos (2007)Time-resolved step-scan FTIR (TRS2-FTIR) and density functional theory have been applied to probe the structural dynamics of CuB in heme-copper oxidases at room temperature. The TRS2-FTIR data of cbb3 from Pseudomonas ...
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Resonance Raman detection of a ferrous five-coordinate nitrosylheme b3 complex in cytochrome cbb3 oxidase from Pseudomonas stutzeri
Pinakoulaki, Eftychia; Stavrakis, Stavros; Urbani, A.; Varotsis, Constantinos (2002)Understanding the chemical nature of the nitric oxide (NO) moiety of nitrosylheme copper oxidases is crucial for elucidation of the NO activation process. In the present work, direct resonance Raman spectroscopic observation ...
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Structural dynamics of heme-copper oxidases and nitric oxide reductases: Time-resolved step-scan Fourier transform infrared and time-resolved resonance Raman studies
Pinakoulaki, Eftychia; Koutsoupakis, Constantinos; Stavrakis, Stavros; Aggelaki, Marialena; Papadopoulos, George Angelos; Daskalakis, Vangelis; Varotsis, Constantinos (2005)Of the spectroscopic methods available for the characterization of the dynamics of heme protein active sites, time-resolved resonance Raman spectroscopy (TR3) is a powerful technique because excitation within the heme π-π* ...
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Time-resolved step-scan Fourier transform infrared investigation of heme-copper oxidases: Implications for O2 input and H 2O/H+ output channels
Koutsoupakis, Constantinos; Pinakoulaki, Eftychia; Stavrakis, Stavros; Daskalakis, Vangelis; Varotsis, Constantinos (2004)We have applied FTIR and time-resolved step-scan Fourier transform infrared (TRS2-FTIR) spectroscopy to investigate the dynamics of the heme-CuB binuclear center and the protein dynamics of mammalian aa3, Pseudomonas ...