Browsing by Subject "Embryo, Nonmammalian"

Article

Activation of endogenous FAK via expression of its amino terminal domain in xenopus embryos

Petridou, Nicoletta I.; Stylianou, Panayiota; Christodoulou, Neophytos; Rhoads, Daniel S.; Guan, Junlin; Skourides, Paris A. (2012)

Background: The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic ...

Article

Cell-Autonomous Ca(2+) Flashes Elicit Pulsed Contractions of an Apical Actin Network to Drive Apical Constriction during Neural Tube Closure

Christodoulou, Neophytos; Skourides, Paris A. (2015)

Neurulation is a critical period in all vertebrates and results in the formation of the neural tube, which gives rise to the CNS. Apical constriction is one of the fundamental morphogenetic movements that drives neural ...

Article

Comparing intracellular stability and targeting of sulfobetaine quantum dots with other surface chemistries in live cells

Muro, E.; Fragola, A.; Pons, T.; Lequeux, N.; Ioannou, Androulla; Skourides, Paris A.; Dubertret, B. (2012)

The in vivo labeling of intracellular components with quantum dots (QDs) is very limited because of QD aggregation in the cell cytoplasm and/or QD confinement into lysosomal compartments. In order to improve intracellular ...

Article

Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis

Pitsouli, Chrysoula; Perrimon, N. (2010)

Adult structures in holometabolous insects such as Drosophila are generated by groups of imaginal cells dedicated to the formation of different organs. Imaginal cells are specified in the embryo and remain quiescent until ...

Article

FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis

Petridou, Nicoletta I.; Skourides, Paris A. (2014)

Spindle orientation is critical for proper morphogenesis of organs and tissues as well as for the maintenance of tissue morphology. Although significant progress has been made in understanding the mechanisms linking the ...

Article

In vivo imaging of quantum dots encapsulated in phospholipid micelles

Dubertret, B.; Skourides, Paris A.; Norris, D. J.; Brivanlou, A. H.; Libchaber, A.; Noireaux, V. (2002)

Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this ...

Article

Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo

Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2011)

Background: Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within ...