Browsing by Subject "Plasmids"
Now showing items 1-5 of 5
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Article
Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes
(2008)A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase ...
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Article
Flavonoids as DNA topoisomerase antagonists and poisons: Structure-activity relationships
(1995)Selected flavonoids were tested for their ability to inhibit the catalytic activity of DNA topoisomerase (topo) I and II. Myricetin, quercetin, fisetin, and morin were found to inhibit both enzymes, while phloretin, ...
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Nanoscopic cationic methacrylate star homopolymers: Synthesis by group transfer polymerization, characterization and evaluation as transfection reagents
(2004)Seven star polymers with degrees of polymerization (DPs) of the arms from 10 to 100 and dimensions in the nanometer range were prepared using sequential group transfer polymerization of 2-(dimethylamino)-ethyl methacrylate ...
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Article
Topologically constrained domains of supercoiled DNA in eukaryotic cells
(1997)The size of supercoiled, topologically constrained DNA domains within the squamous carcinoma cell line SQ-20B were determined by direct comparison with a panel of irradiated supercoiled plasmid DNAs. Loss of supercoiling ...
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Article
Trophoblast-specific gene manipulation using lentivirus-based vectors
(2007)The trophoblast layers of the mammalian placenta carry out many complex functions required to pattern the developing embryo and maintain its growth and survival in the uterine environment. Genetic disruption of many gene ...