Browsing by Subject "RNA, Messenger"

Article

A base substitution at IVS-19 3′-end splice junction causes exon 20 skipping in proα2(I) collagen mRNA and produces mild osteogenesis imperfecta

Mottes, M.; Sangalli, A.; Valli, M.; Forlino, A.; Gomez-Lira, M.; Antoniazzi, F.; Constantinou-Deltas, Constantinos D.; Cetta, G.; Pignatti, P. F. (1994)

Molecular investigations on a young patient and her family were undertaken to identify the molecular defect responsible for a mild form of osteogenesis imperfecta (OI) with blue sclerae, dentinogenesis imperfecta and joint ...

Article

Coexpression of H2-Mb and H2-Ab genes during fetal and postnatal development

Georgiades, Pantelis; Kieszkiewicz, J.; Rozycka, M.; Brickell, P. M.; Lund, T. (1996)

The major histocompatibility complex (MHC) class II-like molecules, H2-M, have an essential role in processing and presentation of antigens by the MHC class II molecules, because functional inactivation of these genes lead ...

Article

Delta-like and Gtl2 are reciprocally expressed differentially methylated linked imprinted genes on mouse chromosome 12

Takada, S.; Tevendale, M.; Baker, J.; Georgiades, Pantelis; Campbell, E.; Freeman, T.; Johnson, M. H.; Paulsen, M.; Ferguson-Smith, A. C. (2000)

The distal portion of mouse chromosome 12 is imprinted. To date, however, Gtl2 is the only imprinted gene identified on chromosome 12. Gtl2 encodes multiple alternatively spliced transcripts with no apparent open reading ...

Article

An error in dystrophin mRNA processing in golden retriever muscular dystrophy, an animal homologue of Duchenne muscular dystrophy

Sharp, N. J. H.; Kornegay, J. N.; Van Camp, S. D.; Herbstreith, M. H.; Secore, S. L.; Kettle, S.; Hung, W. -Y; Constantinou-Deltas, Constantinos D.; Dykstra, M. J.; Roses, A. D.; Bartlett, R. J. (1992)

Golden retriever muscular dystrophy (GRMD) is a spontaneous, X-linked, progressively fatal disease of dogs and is also a homologue of Duchenne muscular dystrophy (DMD). Two-thirds of DMD patients carry detectable deletions ...

Article

Genomic approaches that aid in the identification of transcription factor target genes

Kirmizis, Antonis; Farnham, P. J. (2004)

It is well-established that deregulation of the transcriptional activity of many different genes has been causatively linked to human diseases. In cancer, altered patterns of gene expression are often the result of the ...

Article

High-resolution whole-mount in situ hybridization using quantum dot nanocrystals

Ioannou, Androulla; Eleftheriou, Iro; Lubatti, Andrea; Charalambous, Anna; Skourides, Paris A. (2012)

The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically ...

Article

Histone H3 tail clipping regulates gene expression

Santos-Rosa, H.; Kirmizis, Antonis; Nelson, C.; Bartke, T.; Saksouk, N.; Cote, J.; Kouzarides, T. (2009)

Induction of gene expression in yeast and human cells involves changes in the histone modifications associated with promoters. Here we identify a histone H3 endopeptidase activity in Saccharomyces cerevisiae that may ...

Article

Identification of the polycomb group protein SU(Z)12 as a potential molecular target for human cancer therapy

Kirmizis, Antonis; Bartley, S. M.; Farnham, P. J. (2003)

We have previously identified SU(Z)12 as an E2F target gene. Because many E2F target genes encode proteins that are critical for the control of cell proliferation, we have further characterized the regulation and expression ...

Article

In vivo, site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing

Charalambous, Anna; Andreou, Maria I.; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2012)

The ability to target proteins with nanostructures and/or nanodevices in vivo is important for understanding and controlling their biological function. Quantum dots (QDs) serve as an ideal model nanostructure due to their ...

Article

KIF1Bβ transports dendritically localized mRNPs in neurons and is recruited to synapses in an activity-dependent manner

Charalambous, Despina C.; Pasciuto, E.; Mercaldo, V.; Pilo Boyl, P.; Munck, S.; Bagni, C.; Santama, Niovi (2013)

KIF1Bβ is a kinesin-like, microtubule-based molecular motor protein involved in anterograde axonal vesicular transport in vertebrate and invertebrate neurons. Certain KIF1Bβ isoforms have been implicated in different forms ...

Article

Regulation of Lactate Dehydrogenase Gene Expression by AMP‐dependent Protein Kinase Subunits

JUNGMANN, R. A.; Constantinou, Andreas I.; SQUINTO, S. P.; KWAST‐WELFELD, J.; SCHWEPPE, J. S. (1986)

Article

Rotenoids mediate potent cancer chemopreventive activity through transcriptional regulation of ornithine decarboxylasetissue plasminogen activator-mediated thrombolysis

Gerhäuser, C.; Mar, W.; Lee, S. K.; Suh, N.; Luo, Y.; Kosmeder, J.; Luyengi, L.; Fong, H. H. S.; Kinghorn, A. D.; Moriarty, R. M.; Mehta, R. G.; Constantinou, Andreas I.; Moon, R. C.; Pezzuto, J. M. (1995)

For the discovery of new cancer chemopreventive agents, we have studied the potential of plant extracts to inhibit phorbol ester-induced ornithine decarboxylase (ODC) activity in cell culture. Four active rotenoids were ...

Article

Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells

Tonetti, D. A.; Rubenstein, R.; DeLeon, M.; Zhao, H.; Pappas, S. G.; Bentrem, D. J.; Chen, B.; Constantinou, Andreas I.; Jordan, V. C. (2003)

We previously reported stable transfection of estrogen receptor alpha (ERα) into the ER-negative MDA-MB-231 cells (S30) as a tool to examine the mechanism of action of estrogen and antiestrogens [J. Natl. Cancer Inst. 84 ...

Article

Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy

Lewin, S. R.; Vesanen, M.; Kostrikis, Leontios G.; Hurley, A.; Duran, M.; Zhang, L.; Ho, David D.; Markowitz, M.; Gibson, U. E.; Heid, C. A. (1999)

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR ...