Browsing by Subject "animal experiment"

Article

Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells

Antoniades, Ioanna; Stylianou, Panayiota; Christodoulou, Neophytos; Skourides, Paris A. (2017)

We previously identified focal adhesion kinase (FAK) as an important regulator of ciliogenesis in multiciliated cells. FAK and other focal adhesion (FA) proteins associate with the basal bodies and their striated rootlets ...

Article

Angiotensin inhibition enhances drug delivery and potentiates chemotherapy by decompressing tumour blood vessels

Chauhan, V. P.; Martin, J. D.; Liu, H.; Lacorre, D. A.; Jain, S. R.; Kozin, S. V.; Stylianopoulos, T.; Mousa, A. S.; Han, X.; Adstamongkonkul, P.; Popovic, Z.; Huang, P.; Bawendi, M. G.; Boucher, Y.; Jain, R. K. (2013)

Cancer and stromal cells actively exert physical forces (solid stress) to compress tumour blood vessels, thus reducing vascular perfusion. Tumour interstitial matrix also contributes to solid stress, with hyaluronan ...

Article

Bacterial cupredoxin azurin as an inducer of apoptosis and regression in human breast cancer

Punj, V.; Bhattacharyya, S.; Saint-Dic, D.; Vasu, C.; Cunningham, E. A.; Graves, J.; Yamada, T.; Constantinou, Andreas I.; Christov, K.; White, B.; Li, G.; Majumdar, D.; Chakrabarty, A. M.; Das Gupta, T. K. (2004)

Azurin, a copper-containing redox protein released by the pathogenic bacterium Pseudomonas aeruginosa, is highly cytotoxic to the human breast cancer cell line MCF-7, but is less cytotoxic toward p53-negative (MDA-MB-157) ...

Article

Beneficial effects of a vanadium complex with cysteine, administered at low doses on benzo(α) pyrene-induced leiomyosarcomas in wistar rats

Liasko, Roman; Kabanos, Themistoklis A.; Karkabounas, Spyridon Ch; Malamas, Michael Ph; Tasiopoulos, Anastasios J.; Stefanou, Dimitrios G.; Collery, P.; Evangelou, Angelos M. (1998)

Background: Vanadium is a potent environmental and body metal, possessing remarkable antitumor and antidiabetic properties. Vanadium salts and complexes have been widely investigated for their anticarcinogenic properties ...

Article

Calpain2 protease: A new member of the Wnt/Ca2+ pathway modulating convergent extension movements in Xenopus

Zanardelli, Sara; Christodoulou, Neophytos; Skourides, Paris A. (2013)

Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate several physiological processes by limited cleavage of different substrates. The role of Calpain2 in embryogenesis is not clear with ...

Article

Character displacement of song and morphology in African tinkerbirds

Kirschel, A. N. G.; Blumstein, Daniel T.; Smith, T. B. (2009)

Divergence in acoustic signals between populations of animals can lead to species recognition failure, reproductive isolation, and speciation. Character displacement may facilitate coexistence of species in natural ...

Article

Chemopreventive effects of soy protein and purified soy isoflavones on DMBA-induced mammary tumors in female Sprague-Dawley rats

Constantinou, Andreas I.; Lantvit, D.; Hawthorne, M.; Xu, X.; Van Breemen, R. B.; Pezzuto, J. M. (2001)

There are conflicting reports on the effect of soy and its components on mammary carcinogenesis in adult female rats, mainly because of different rodent models that are used in chemoprevention studies. The present study ...

Article

Combined chemical and optical methods for monitoring the early decay stages of surrogate human models

Statheropoulos, Miltiades K.; Agapiou, Agapios; Zorba, Eleni; Mikedi, Katerina; Karma, Sofia; Pallis, George C.; Eliopoulos, C.; Spiliopoulou, C. (2011)

As the body decays shortly after death, a variety of gases and volatile organic compounds (VOCs) constantly emanate. Ethical and practical reasons limit the use of human corpses in controlled, time-dependent, intervening ...

Article

Cyst formation in the PKD2 (1-703) transgenic rat precedes deregulation of proliferation-related pathways

Koupepidou, P.; Felekkis, Kyriacos N.; Kränzlin, B.; Sticht, C.; Gretz, N.; Constantinou-Deltas, Constantinos D. (2010)

Background: Polycystic Kidney Disease is characterized by the formation of large fluid-filled cysts that eventually destroy the renal parenchyma leading to end-stage renal failure. Although remarkable progress has been ...

Article

Deficiencies in the Fanconi Anemia DNA damage response pathway increase sensitivity to HPV-associated head and neck cancer

Park, J. W.; Pitot, H. C.; Strati, K.; Spardy, N.; Duensing, S.; Grompe, M.; Lambert, P. F. (2010)

Patients with the rare genetic disease, Fanconi anemia (FA), are highly susceptible to squamous cell carcinomas arising at multiple anatomic sites including the head and neck region. Human papillomaviruses (HPVs), particularly ...

Article

Dietary retinoids and carotenoids in rodent models of mammary tumorigenesis

Moon, R. C.; Constantinou, Andreas I. (1997)

In this review of the scientific literature the relationship between retinoids, carotenoids, and mammary carcinogenesis is examined. Several retinoids have shown promise as chemopreventive agents against chemically induced ...

Article

Dietary Soy Isoflavones and Estrone Protect Ovariectomized ERαKO and Wild-Type Mice from Carcinogen-Induced Colon Cancer

Guo, J. -Y; Li, X.; Browning Jr., J. D.; Rottinghaus, G. E.; Lubahn, D. B.; Constantinou, Andreas I.; Bennink, M.; MacDonald, R. S. (2004)

Consumption of soy foods has been weakly associated with reduced colon cancer risk. Colon cancer risk is influenced by estrogen exposure, although the mechanism through which this occurs is not defined. Conversion of ...

Article

Distribution of epidermal growth factor receptors in normal and neoplastic mammary tissues

Mehta, R. G.; Mehta, R. R.; Constantinou, Andreas I.; Moon, R. C. (1995)

Epidermal growth factor (EGF) is considered to be mitogenic for proliferation of mammary glands in animals. The action of EGF is mediated by specific EGF receptors (EGF-R). In the present study, we investigated distribution ...

Article

Down-regulation of glutatione S-transferase α 4 (hGSTA4) in the muscle of thermally injured patients is indicative of susceptibility to bacterial infection

Apidianakis, Yiorgos; Que, Y. -A; Xu, W.; Tegos, G. P.; Zimniak, P.; Hamblin, M. R.; Tompkins, R. G.; Xiao, W.; Rahme, L. G. (2012)

Patients with severe burns are highly susceptible to bacterial infection. While immunosuppression facilitates infection, the contribution of soft tissues to infection beyond providing a portal for bacterial entry remains ...

Article

Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques

Jin, X.; Bauer, D. E.; Tuttleton, S. E.; Lewin, S.; Gettie, A.; Blanchard, J.; Irwin, C. E.; Safrit, J. T.; Mittler, J.; Weinberger, L.; Kostrikis, Leontios G.; Zhang, L.; Perelson, A. S.; Ho, David D. (1999)

To determine the role of CD8+ T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was ...

Article

Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa infection

Apidianakis, Yiorgos; Rahme, L. G. (2009)

Conservation of host signaling pathways and tissue physiology between Drosophila melanogaster and mammals allows for the modeling of human host-pathogen interactions in Drosophila. Here we present the use of genetically ...

Article

The effect of the phytoestrogens genistein, daidzein, and equol on the growth of tamoxifen-resistant T47D/PKCα

Tonetti, D. A.; Zhang, Y.; Zhao, H.; Lim, S. -B; Constantinou, Andreas I. (2007)

Soy supplements are often consumed by women for alleviating menopausal symptoms or for the perceived protective effects against breast cancer. More concerning is the concurrent consumption of soy isoflavones with tamoxifen ...

Article

Effects of in Vivo CD8+ T cell depletion on virus replication in rhesus macaques immunized with a live, attenuated simian immunodeficiency virus vaccine

Metzner, K. J.; Jin, X.; Lee, F. V.; Gettie, A.; Bauer, D. E.; Di Mascio, M.; Perelson, A. S.; Marx, P. A.; Ho, David D.; Kostrikis, Leontios G.; Connor, R. I. (2000)

The role of CD8+ T lymphocytes in controlling replication of live, attenuated simian immunodeficiency virus (SIV) was investigated as part of a vaccine study to examine the correlates of protection in the SIV/rhesus macaque ...

Article

Effects of sol-gel synthesis on 5Fe-15Mn-40Zn-40Ti-O mixed oxide structure and its H2S removal efficiency from industrial gas streams

Polychronopoulou, Kyriaki; Efstathiou, Angelos M. (2009)

A novel Fe-Mn-Zn-Ti-O mixed metal oxide has been developed for efficient low-temperature (25-50°C) removal of H2S from a gas mixture containing 600 ppm H2S, 25 vol% H2, 7.5 vol % CO 2, and 1-3 vol% H2O that simulates typical ...

Article

FOXO3a is broadly neuroprotective in vitro and in vivo against insults implicated in motor neuron diseases

Mojsilovic-Petrovic, J.; Nedelsky, N.; Boccitto, M.; Mano, I.; Georgiades, Savvas N.; Zhou, W.; Liu, Y.; Neve, R. L.; Taylor, J. P.; Driscoll, M.; Clardy, J.; Merry, D.; Kalb, R. G. (2009)

Aging is a risk factor for the development of adult-onset neurodegenerative diseases. Although some of the molecular pathways regulating longevity and stress resistance in lower organisms are defined (i.e., those activating ...