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Browsing by Subject "immunoprecipitation"

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    • Article  

      Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells 

      Antoniades, Ioanna; Stylianou, Panayiota; Christodoulou, Neophytos; Skourides, Paris A. (2017)
      We previously identified focal adhesion kinase (FAK) as an important regulator of ciliogenesis in multiciliated cells. FAK and other focal adhesion (FA) proteins associate with the basal bodies and their striated rootlets ...

    • Article  

      Altered Phosphorylation, Biosynthesis and Degradation of the 170 kDa Isoform of Topoisomerase II in Amsacrine-Resistant Human Leukemia Cells 

      Ganapathi, R.; Zwelling, L.; Constantinou, Andreas I.; Ford, J.; Grabowski, D. (1993)
      Resistance to amsacrine in HL-60/AMSA is 50-100 fold compared to the parental HL-60/S cells. Synthesis and phosphorylation of topoisomerase II (TOPO II) were 2-3 fold lower in HL-60/AMSA compared to HL-60/S cells metabolically ...

    • Article  

      Arginine methylation at histone H3R2 controls deposition of H3K4 trimethylation 

      Kirmizis, Antonis; Santos-Rosa, H.; Penkett, C. J.; Singer, M. A.; Vermeulen, M.; Mann, M.; Bähler, J.; Green, R. D.; Kouzarides, T. (2007)
      Modifications on histones control important biological processes through their effects on chromatin structure. Methylation at lysine 4 on histone H3 (H3K4) is found at the 5′ end of active genes and contributes to ...

    • Article  

      Fetal-specific DNA methylation ratio permits noninvasive prenatal diagnosis of trisomy 21 

      Papageorgiou, Elisavet A.; Karagrigoriou, Alex; Tsaliki, Evdokia; Velissariou, Voula; Carter, N. P.; Patsalis, Philippos C. (2011)

    • Article  

      A functional variant in NEPH3 gene confers high risk of renal failure in primary hematuric glomerulopathies. Evidence for predisposition to microalbuminuria in the general population 

      Voskarides, Konstantinos; Stefanou, Charalambos; Pieri, Myrtani; Demosthenous, Panayiota; Felekkis, Kyriacos N.; Arsali, Maria; Athanasiou, Yiannis; Xydakis, D.; Stylianou, Konstantinos G.; Daphnis, Eugenios K.; Goulielmos, George N.; Loizou, P.; Savige, J.; Höhne, M.; Völker, L. A.; Benzing, T.; Maxwell, P. H.; Gale, D. P.; Gorski, M.; Böger, C.; Kollerits, B.; Kronenberg, F.; Paulweber, B.; Zavros, Michalis; Pierides, Alkis M.; Constantinou-Deltas, Constantinos D. (2017)
      Background Recent data emphasize that thin basement membrane nephropathy (TBMN) should not be viewed as a form of benign familial hematuria since chronic renal failure (CRF) and even end-stage renal disease (ESRD), is a ...

    • Article  

      Genomic approaches that aid in the identification of transcription factor target genes 

      Kirmizis, Antonis; Farnham, P. J. (2004)
      It is well-established that deregulation of the transcriptional activity of many different genes has been causatively linked to human diseases. In cancer, altered patterns of gene expression are often the result of the ...

    • Article  

      KIF1Bβ transports dendritically localized mRNPs in neurons and is recruited to synapses in an activity-dependent manner 

      Charalambous, Despina C.; Pasciuto, E.; Mercaldo, V.; Pilo Boyl, P.; Munck, S.; Bagni, C.; Santama, Niovi (2013)
      KIF1Bβ is a kinesin-like, microtubule-based molecular motor protein involved in anterograde axonal vesicular transport in vertebrate and invertebrate neurons. Certain KIF1Bβ isoforms have been implicated in different forms ...

    • Article  

      MeDIP real-time qPCR of maternal peripheral blood reliably identifies trisomy 21 

      Tsaliki, Evdokia; Papageorgiou, Elisavet A.; Spyrou, Christiana; Koumbaris, George L.; Kypri, Elena; Kyriakou, Skevi; Sotiriou, Chrysovalanto; Touvana, Evi; Keravnou, Anna; Karagrigoriou, Alex; Lamnissou, Klea; Velissariou, Voula; Patsalis, Philippos C. (2012)
      Objective: To reevaluate the efficiency of the 12 differentially methylated regions (DMRs) used in the methylated DNA immunoprecipitation (MeDIP) real-time quantitative polymerase chain reaction (real-time qPCR) based ...

    • Article  

      A new non-invasive prenatal diagnosis of Down syndrome through epigenetic markers and real-time qPCR 

      Patsalis, Philippos C.; Tsaliki, Evdokia; Koumbaris, George L.; Karagrigoriou, Alex; Velissariou, Voula; Papageorgiou, Elisavet A. (2012)
      Introduction: Non-invasive prenatal diagnosis (NIPD) of Down syndrome is rapidly evolving. Currently, two applications for NIPD of Down syndrome have been developed with potential and have displayed positive results

    • Article  

      Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27 

      Kirmizis, Antonis; Bartley, S. M.; Kuzmichev, A.; Margueron, R.; Reinberg, D.; Green, R.; Farnham, P. J. (2004)
      Polycomb group (PcG) complexes 2 and 3 are involved in transcriptional silencing. These complexes contain a histone lysine methyltransferase (HKMT) activity that targets different lysine residues on histones H1 or H3 in ...

    • Article  

      WR-1065, an active metabolite of the cytoprotector amifostine, affects phosphorylation of topoisomerase IIα leading to changes in enzyme activity and cell cycle progression in CHO AA8 cells 

      Murley, J. S.; Constantinou, Andreas I.; Kamath, N. S.; Grdina, D. J. (1997)
      The effects of WR-1065 (2-((aminopropyl)amino)ethanethiol) on cell cycle progression, topoisomerase (topo) IIα activity, and topo IIα phosphorylation in Chinese hamster ovary (CHO) cells have been investigated. Exposure ...

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