Browsing by Subject "in vitro study"

Article

The A and B fragments of normal type I procollagen have a similar thermal stability to proteinase digestion but are selectively destabilized by structural mutations

Constantinou-Deltas, Constantinos D.; VOGEL, B. E.; JEFFREY, J. J.; PROCKOP, D. J. (1987)

Previous studies demonstrated that the thermal stability of the procollagen triple helix can be assayed by digesting the protein for short periods with high concentrations of trypsin and chymotrypsin. Here we cleaved human ...

Article

Ets2 is necessary in trophoblast for normal embryonic anteroposterior axis development

Georgiades, Pantelis; Rossant, J. (2006)

Although the trophoblast is necessary for the growth, viability and patterning of the mammalian embryo, understanding of its patterning role is still rudimentary. Expression of the transcription factor Ets2 is restricted ...

Article

FOXO3a is broadly neuroprotective in vitro and in vivo against insults implicated in motor neuron diseases

Mojsilovic-Petrovic, J.; Nedelsky, N.; Boccitto, M.; Mano, I.; Georgiades, Savvas N.; Zhou, W.; Liu, Y.; Neve, R. L.; Taylor, J. P.; Driscoll, M.; Clardy, J.; Merry, D.; Kalb, R. G. (2009)

Aging is a risk factor for the development of adult-onset neurodegenerative diseases. Although some of the molecular pathways regulating longevity and stress resistance in lower organisms are defined (i.e., those activating ...

Article

Hyaluronan-Derived Swelling of Solid Tumors, the Contribution of Collagen and Cancer Cells, and Implications for Cancer Therapy

Voutouri, C.; Polydorou, C.; Papageorgis, P.; Gkretsi, V.; Stylianopoulos, T. (2016)

Despite the important role that mechanical forces play in tumor growth and therapy, the contribution of swelling to tumor mechanopathology remains unexplored. Tumors rich in hyaluronan exhibit a highly negative fixed charge ...

Article

Identification of Ras suppressor-1 (RSU-1) as a potential breast cancer metastasis biomarker using a three-dimensional in vitro approach

Gkretsi, V.; Stylianou, A.; Louca, M.; Stylianopoulos, T. (2017)

Breast cancer (BC) is the most common malignant disease in women, with most patients dying from metastasis to distant organs, making discovery of novel metastasis biomarkers and therapeutic targets imperative. Extracellular ...

Article

Identification of the factors responsible for the in vitro pro-oxidant and cytotoxic activities of the olive polyphenols oleuropein and hydroxytyrosol

Odiatou, Elena M.; Skaltsounis, Alexios Leandros; Constantinou, Andreas I. (2013)

The olive polyphenols oleuropein and hydroxytyrosol were reported recently to produce extracellular hydrogen peroxide (H2O2) under standard culture conditions. The precise factors responsible for this production and the ...

Article

Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals

Stylianou, Panayiota; Skourides, Paris A. (2009)

Mesoderm migration is a well studied morphogenetic movement that takes place during Xenopus gastrulation. The study of mesoderm migration and other morphogenetic movements has been primarily based on in vitro assays due ...

Article

In vitro antioxidant activity and phenolic content of Cedrus brevifolia bark

Cretu, E.; Salminen, J. -P; Karonen, M.; Miron, A.; Charalambous, Christina; Constantinou, Andreas I.; Aprotosoaie, A. C. (2014)

A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The ...

Article

In vitro study on the antioxidant activity of a polyphenol-rich extract from pinus brutia bark and its fractions

Cretu, E.; Karonen, M.; Salminen, J. -P; Mircea, C.; Trifan, A.; Charalambous, Christina; Constantinou, Andreas I.; Miron, A. (2013)

A crude hydromethanolic extract from Pinus brutia bark and its fractions (diethyl ether, ethyl acetate, n-butanol, and aqueous fractions) were studied with regard to their phenolic content and antioxidant activities. The ...

Article

Multiscale biphasic modelling of peritumoural collagen microstructure: The effect of tumour growth on permeability and fluid flow

Wijeratne, P. A.; Hipwell, J. H.; Hawkes, D. J.; Stylianopoulos, T.; Vavourakis, V. (2017)

We present an in-silico model of avascular poroelastic tumour growth coupled with a multiscale biphasic description of the tumour–host environment. The model is specified to in-vitro data, facilitating biophysically realistic ...

Article

Multistage nanoparticle delivery system for deep penetration into tumor tissue

Wong, C.; Stylianopoulos, T.; Cui, J.; Martin, J.; Chauhan, V. P.; Jiang, W.; Popovic, Z.; Jain, R. K.; Bawendi, M. G.; Fukumura, D. (2011)

Current Food and Drug Administration-approved cancer nanotherapeutics, which passively accumulate around leaky regions of the tumor vasculature because of an enhanced permeation and retention (EPR) effect, have provided ...

Article

Multistage nanoparticles for improved delivery into tumor tissue

Stylianopoulos, T.; Wong, C.; Bawendi, M. G.; Jain, R. K.; Fukumura, D. (2012)

The enhanced permeability and retention (EPR) effect has been a key rationale for the development of nanoscale carriers to solid tumors. As a consequence of EPR, nanotherapeutics are expected to improve drug and detection ...

Article

New compstatin peptides containing n-terminal extensions and non-natural amino acids exhibit potent complement inhibition and improved solubility characteristics

Gorham, R. D.; Forest, D. L.; Khoury, G. A.; Smadbeck, J.; Beecher, C. N.; Healy, E. D.; Tamamis, Phanourios; Archontis, Georgios Z.; Larive, C. K.; Floudas, C. A.; Radeke, M. J.; Johnson, L. V.; Morikis, D. (2015)

Compstatin peptides are complement inhibitors that bind and inhibit cleavage of complement C3. Peptide binding is enhanced by hydrophobic interactions

Article

Novel compstatin family peptides inhibit complement activation by drusen-like deposits in human retinal pigmented epithelial cell cultures

Gorham, R. D.; Forest, D. L.; Tamamis, Phanourios; López de Victoria, A.; Kraszni, M.; Kieslich, C. A.; Banna, C. D.; Bellows-Peterson, M. L.; Larive, C. K.; Floudas, C. A.; Archontis, Georgios Z.; Johnson, L. V.; Morikis, D. (2013)

We have used a novel human retinal pigmented epithelial (RPE) cell-based model that mimics drusen biogenesis and the pathobiology of age-related macular degeneration to evaluate the efficacy of newly designed peptide ...

Article

Novel mixed metal Ag(I)-Sb(III)-metallotherapeutics of the NSAIDs, aspirin and salicylic acid: Enhancement of their solubility and bioactivity by using the surfactant CTAB

Gkaniatsou, E. I.; Banti, Christina N.; Kourkoumelis, Nikolaos; Skoulika, S.; Manoli, Maria; Tasiopoulos, Anastasios J.; Hadjikakou, Sotiris K. (2015)

The already known Ag(I)-Sb(III) compound of the formula {Ag(Ph3Sb)3(NO3)} (1) and two novel mixed metal Ag(I)-Sb(III) metallotherapeutics of the formulae {Ag(Ph3Sb)3(SalH)}(2) and {Ag(Ph3Sb)3(Asp)}(3) (SalH2 = salicylic ...

Article

Phenoxodiol, an anticancer isoflavene, induces immunomodulatory effects in vitro and in vivo

Georgaki, Sylvianna; Skopeliti, Margarita; Tsiatas, Marinos L.; Nicolaou, Katerina A.; Ioannou, Kyriaki; Husband, A.; Bamias, Aristotelis T.; Dimopoulos, Meletios A.; Constantinou, Andreas I.; Tsitsilonis, Ourania E. (2009)

Phenoxodiol (PXD) is a synthetic analogue of the plant isoflavone genistein with improved anticancer efficacy. Various properties and mechanisms of action have been attributed to the drug, the most important being its ...

Article

Regional aerosol deposition in the human airways: The SimInhale benchmark case and a critical assessment of in silico methods

Koullapis, P.; Kassinos, Stavros C.; Muela, J.; Perez-Segarra, C.; Rigola, J.; Lehmkuhl, O.; Cui, Y.; Sommerfeld, M.; Elcner, J.; Jicha, M.; Saveljic, I.; Filipovic, N.; Lizal, F.; Nicolaou, L. (2018)

Regional deposition effects are important in the pulmonary delivery of drugs intended for the topical treatment of respiratory ailments. They also play a critical role in the systemic delivery of drugs with limited lung ...

Article

Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo

Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2011)

Background: Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within ...

Article

Structure based inhibitor design targeting glycogen phosphorylase b. Virtual screening, synthesis, biochemical and biological assessment of novel N-acyl-β-d-glucopyranosylamines

Parmenopoulou, Vanessa; Kantsadi, Anastassia L.; Tsirkone, Vicky G.; Chatzileontiadou, Demetra S. M.; Manta, Stella; Zographos, Spyros E.; Molfeta, Christina; Archontis, Georgios Z.; Agius, Loranne; Hayes, Joseph M.; Leonidas, Demetres D.; Komiotis, Dimitris (2014)

Glycogen phosphorylase (GP) is a validated target for the development of new type 2 diabetes treatments. Exploiting the Zinc docking database, we report the in silico screening of 1888 N-acyl-β-d-glucopyranosylamines ...

Article

Study on single crystal structure of the antimony(III) bromide complex with 3-methyl-2-mercaptobenzothiazole and biological activity of some antimony(III) bromide complexes with thioamides

Ozturk, I. I.; Metsios, A. K.; Filimonova-Orlova, S.; Kourkoumelis, Nikolaos; Hadjikakou, Sotiris K.; Manos, Manolis J.; Tasiopoulos, Anastasios J.; Karkabounas, Spyridon Ch; Milaeva, E. R.; Hadjiliadis, Nick (2012)

Crystals of the [SbBr3(MMBZT)2] (1) complex (MMBZT = N-methyl-2- mercaptobenzothiazole) were grown from acetonitrile/methanol of the filtrate derived from the reaction between 3-methyl-2-mercaptobenzo thiazole (MMBZT) in ...