Browsing by Subject "isolation and purification"
Now showing items 1-16 of 16
-
Article
Activated biochar derived from cactus fibres - Preparation, characterization and application on Cu(II) removal from aqueous solutions
(2014)The adsorption efficiency of activated biochar prepared from cactus fibres regarding the removal of Cu(II) from aqueous solutions has been investigated as a function of various physicochemical parameters (e.g. pH, initial ...
-
Article
Black cohosh (Cimicifuga racemosa L.) protects against menadione-induced DNA damage through scavenging of reactive oxygen species: Bioassay-directed isolation and characterization of active principles
(2002)The roots/rhizomes of Cimicifuga racemosa L. (Nutt.) (black cohosh) have traditionally been used to treat menopausal symptoms through an unknown mechanism of action. In an effort to determine if black cohosh had additional ...
-
Article
Combined use of cyclofructans and an amino acid ester-based ionic liquid for the enantioseparation of huperzine A and coumarin derivatives in CE
(2015)Cyclofructans (CFs) and their derivatives have recently been proven to be efficient chiral selectors (CSs) for the enantioseparation of several analytes in CE, HPLC, and GC. In this study, the chiral separation ability of ...
-
Article
Combined use of l-alanine tert butyl ester lactate and trimethyl-ß-cyclodextrin for the enantiomeric separations of 2-arylpropionic acids nonsteroidal anti-inflammatory drugs
(2015)In this study, a new CE method, employing a binary system of trimethyl-ß-CD (TM-ß-CD) and a chiral amino acid ester-based ionic liquid (AAIL), was developed for the chiral separation of seven 2-arylpropionic acid nonsteroidal ...
-
Article
Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): A general methodology for detecting rapidly mutating viruses
(2011)Emerging infectious diseases have caused a global effort for development of fast and accurate detection techniques. The rapidly mutating nature of viruses presents a major difficulty, highlighting the need for specific ...
-
Article
Evaluation of amino acid ester-based ionic liquids as buffer additives in CE for the separation of 2-arylpropionic acids nonsteroidal anti-inflammatory drugs
(2014)The aim of the present study is the CE performance evaluation for the separation of 2-arylpropionic acid nonsteroidal anti-inflammatory drugs. In particular, the separation of indoprofen, carprofen, ketoprofen, ibuprofen, ...
-
Article
Genetic analysis of HIV type 1 strains from newly infected untreated patients in cyprus: high genetic diversity and low prevalence of drug resistance.
(2009)Abstract The molecular epidemiology of HIV-1 infection was first studied in Cyprus in the mid-1990s, but the extent of HIV-1 diversity and the prevalence of drug resistance have remained elusive. In an effort to address ...
-
Article
In vivo, site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing
(2012)The ability to target proteins with nanostructures and/or nanodevices in vivo is important for understanding and controlling their biological function. Quantum dots (QDs) serve as an ideal model nanostructure due to their ...
-
Article
Induction of caspase-independent programmed cell death by vitamin E natural homologs and synthetic derivatives.
(2009)Current observations in the literature suggest that vitamin E may be a suitable candidate for cancer chemotherapy. To investigate this further, we examined the ability of the vitamin E natural homologs [alpha-, beta-, ...
-
Article
Isolation of isoflavones from soy-based fermentations of the erythromycin-producing bacterium Saccharopolyspora erythraea
(1997)A search for an abundant and economical source of isoflavones, particularly genistein, led to the discovery that the erythromycin-producing organism Saccharopolyspora erythraea also produces this promising new cancer-prevention ...
-
Article
KIF2β, a new kinesin superfamily protein in non-neuronal cells, is associated with lysosomes and may be implicated in their centrifugal translocation
(1998)Lysosomes concentrate juxtanuclearly in the region around the microtubule-organizing center by interaction with microtubules. Different experimental and physiological conditions can induce these organelles to move to the ...
-
Article
Molecular beacon-based real-time PCR detection of primary isolates of salmonella typhimurium and salmonella enteritidis in environmental and clinical samples
(2009)Background. A fast and simple two-step multiplex real-time PCR assay has been developed to replace the traditional, laborious Salmonella serotyping procedure. Molecular beacons were incorporated into the assay as probes ...
-
Article
Molecular epidemiology of hepatitis C infection in cyprus: Evidence of polyphyletic infection
(2009)The genetic diversity of the hepatitis C virus (HCV) in Cyprus is investigated for the first time in this study. Nucleotide sequence analysis of the CORE-E1 and NS5B regions of the HCV genome was performed on blood plasma ...
-
Article
Permeation profiles of potential urine-borne biomarkers of human presence over brick and concrete
(2012)Headspace solid phase micro-extraction gas chromatography-mass spectrometry (SPME-GC-MS) analysis was performed over an in-house made filling chamber loaded with brick or concrete, mimicking a potential entrapment scene ...
-
Article
Separation of acetylcholinesterase inhibitors using polymeric surfactants in polyelectrolyte multilayer coatings
(2012)The main objective of this study is the use of polymeric surfactants in polyelectrolyte multilayer (PEM) coatings for the separation of the pharmaceutical substances acetylcholinesterase inhibitors (AChEIs). AChEIs are ...
-
Article
Simultaneous determination of nine acetylcholinesterase inhibitors using micellar electrokinetic chromatography
(2011)MEKC was used for the separation of nine acetylcholinesterase inhibitors (AChEIs). AChEIs are an important group of drug compounds that are used medicinally to treat Alzheimer's disease and Myasthenia Gravis. At the time ...