Browsing by Subject "protein expression"

Article

Activation of endogenous FAK via expression of its amino terminal domain in xenopus embryos

Petridou, Nicoletta I.; Stylianou, Panayiota; Christodoulou, Neophytos; Rhoads, Daniel S.; Guan, Junlin; Skourides, Paris A. (2012)

Background: The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic ...

Article

Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells

Antoniades, Ioanna; Stylianou, Panayiota; Christodoulou, Neophytos; Skourides, Paris A. (2017)

We previously identified focal adhesion kinase (FAK) as an important regulator of ciliogenesis in multiciliated cells. FAK and other focal adhesion (FA) proteins associate with the basal bodies and their striated rootlets ...

Article

Angiotensin inhibition enhances drug delivery and potentiates chemotherapy by decompressing tumour blood vessels

Chauhan, V. P.; Martin, J. D.; Liu, H.; Lacorre, D. A.; Jain, S. R.; Kozin, S. V.; Stylianopoulos, T.; Mousa, A. S.; Han, X.; Adstamongkonkul, P.; Popovic, Z.; Huang, P.; Bawendi, M. G.; Boucher, Y.; Jain, R. K. (2013)

Cancer and stromal cells actively exert physical forces (solid stress) to compress tumour blood vessels, thus reducing vascular perfusion. Tumour interstitial matrix also contributes to solid stress, with hyaluronan ...

Article

Bcl-2 blocks 2-methoxyestradiol induced leukemia cell apoptosis by a p27Kip1-dependent G1/S cell cycle arrest in conjunction with NF-κB activation

Batsi, Christina; Markopoulou, Soultana; Kontargiris, Evangelos; Charalambous, Christina; Thomas, Christoforos G.; Christoforidis, Savvas; Kanavaros, Panagiotis E.; Constantinou, Andreas I.; Marcu, Kenneth B.; Kolettas, Evangelos (2009)

2-Methoxyestradiol (2-ME2) induces leukemia cells to undergo apoptosis in association with Bcl-2 inactivation but the mechanisms whereby Bcl-2 contributes to protection against programmed cell death in this context remain ...

Article

Calpain2 protease: A new member of the Wnt/Ca2+ pathway modulating convergent extension movements in Xenopus

Zanardelli, Sara; Christodoulou, Neophytos; Skourides, Paris A. (2013)

Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate several physiological processes by limited cleavage of different substrates. The role of Calpain2 in embryogenesis is not clear with ...

Article

Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation

Kuzmichev, A.; Margueron, R.; Vaquero, A.; Preissner, T. S.; Scher, M.; Kirmizis, Antonis; Ouyang, X.; Brockdorff, N.; Abate-Shen, C.; Farnham, P.; Reinberg, D. (2005)

Changes in the substrate specificities of factors that irreversibly modify the histone components of chromatin are expected to have a profound effect on gene expression through epigenetics. Ezh2 is a histone-lysine ...

Article

CXCR4 mediates entry and productive infection of syncytia-inducing (x4) hiv-1 strains in primary macrophages

Valentin, A.; Trivedi, H.; Lu, W.; Kostrikis, Leontios G.; Pavlakis, G. N. (2000)

CCR5 and CXCR4 are the main coreceptors for non-syncytia-inducing (NSI) and syncytia-inducing (SI) HIV-1 strains, respectively. NSI HIV-1 isolates do not infect either human lymphoid or monocytoid cell lines, and this ...

Article

Defining the role of solid stress and matrix stiffness in cancer cell proliferation and metastasis

Kalli, M.; Stylianopoulos, T. (2018)

Solid tumors are characterized by an abnormal stroma that contributes to the development of biomechanical abnormalities in the tumor microenvironment. In particular, these abnormalities include an increase in matrix stiffness ...

Article

Differential expression of the rat retinoid X receptor γ gene during skeletal muscle differentiation suggests a role in myogenesis

Georgiades, Pantelis; Brickell, P. M. (1997)

Even though previous studies have shown that transcripts encoding the murine retinoid X receptor γ (RXRγ) are present in skeletal muscle of mouse embryos and that cultured myoblasts are induced to differentiate upon retinoid ...

Article

A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis

Petridou, Nicoletta I.; Stylianou, Panayiota; Skourides, Paris A. (2013)

FAK is a non-receptor tyrosine kinase involved in a wide variety of biological processes and crucial for embryonic development. In this manuscript, we report the generation of a new FAK dominant negative (FF), composed of ...

Article

Equol enhances tamoxifen's anti-tumor activity by induction of caspase-mediated apoptosis in MCF-7 breast cancer cells

Charalambous, Christina; Pitta, Chara A.; Constantinou, Andreas I. (2013)

Background: Soy phytoestrogens, such as daidzein and its metabolite equol, have been proposed to be responsible for the low breast cancer rate in Asian women. Since the majority of estrogen receptor positive breast cancer ...

Article

Ets2 is necessary in trophoblast for normal embryonic anteroposterior axis development

Georgiades, Pantelis; Rossant, J. (2006)

Although the trophoblast is necessary for the growth, viability and patterning of the mammalian embryo, understanding of its patterning role is still rudimentary. Expression of the transcription factor Ets2 is restricted ...

Article

Ets2-dependent trophoblast signalling is required for gastrulation progression after primitive streak initiation

Polydorou, Christiana; Georgiades, Pantelis (2013)

Although extraembryonic ectoderm trophoblast signals the embryo for primitive streak initiation, a prerequisite for gastrulation, it is unknown whether it also signals for the progression of gastrulation after primitive ...

Article

FOXO3a is broadly neuroprotective in vitro and in vivo against insults implicated in motor neuron diseases

Mojsilovic-Petrovic, J.; Nedelsky, N.; Boccitto, M.; Mano, I.; Georgiades, Savvas N.; Zhou, W.; Liu, Y.; Neve, R. L.; Taylor, J. P.; Driscoll, M.; Clardy, J.; Merry, D.; Kalb, R. G. (2009)

Aging is a risk factor for the development of adult-onset neurodegenerative diseases. Although some of the molecular pathways regulating longevity and stress resistance in lower organisms are defined (i.e., those activating ...

Article

Groucho mediates a Ci-independent mechanism of hedgehog repression in the anterior wing pouch

Apidianakis, Yiorgos; Grbavec, D.; Stifani, S.; Delidakis, Christos (2001)

Groucho (Gro) is the founding member of a family of transcriptional co-repressors that are recruited by a number of different transcription factors. Drosophila has a single gro gene, whose loss of function affects processes ...

Article

Hedgehog restricts its expression domain in the Drosophila wing

Bejarano, F.; Pérez, L.; Apidianakis, Yiorgos; Delidakis, Christos; Milán, M. (2007)

The stable subdivision of Drosophila limbs into anterior and posterior compartments is a consequence of asymmetrical signalling by Hedgehog (Hh), from the posterior to anterior cells. The activity of the homeodomain protein ...

Article

Human papillomavirus type 16 E6 and E7 oncoproteins act synergistically to cause head and neck cancer in mice

Jabbar, S.; Strati, Katerina; Shin, M. K.; Pitot, H. C.; Lambert, P. F. (2010)

High-risk human papillomaviruses (HPVs) contribute to cervical and other anogenital cancers, and they are also linked etiologically to a subset of head and neck squamous cell carcinomas (HNSCC). We previously established ...

Article

Identification of a potent activator of Akt phosphorylation from a novel series of phenolic, picolinic, pyridino, and hydroxamic zinc(II) complexes

Georgiades, Savvas N.; Mak, L. H.; Angurell, I.; Rosivatz, E.; Firouz Mohd Mustapa, M.; Polychroni, Christoulla; Woscholski, R.; Vilar, R. (2011)

The discovery of small-molecule modulators of signaling pathways is currently a particularly active area of research. We aimed at developing unprecedented metal-based activators of Akt signaling which can potentially find ...

Article

Identification of biomarkers that distinguish human papillomavirus (HPV)-positive versus HPV-negative head and neck cancers in a mouse model

Strati, Katerina; Pitot, H. C.; Lambert, P. F. (2006)

Head and neck squamous cell carcinoma (HNSCC) is a leading cause of cancer mortality worldwide. Recent reports have associated a subset of HNSCC with high-risk human papillomaviruses (HPVs), particularly HPV16, the same ...

Article

The Ink4/Arf locus is a barrier for iPS cell reprogramming

Li, H.; Collado, M.; Villasante, A.; Strati, Katerina; Ortega, S.; Cãamero, M.; Blasco, M. A.; Serrano, M. (2009)

The mechanisms involved in the reprogramming of differentiated cells into induced pluripotent stem (iPS) cells by the three transcription factors Oct4 (also known as Pou5f1), Klf4 and Sox2 remain poorly understood. The ...