Browsing by Subject "protein localization"

Article

40LoVe and Samba are involved in Xenopusneural development and functionally distinct from hnRNP AB

Andreou, Maria I.; Yan, C. Y. I.; Skourides, Paris A. (2014)

Heterogeneous nuclear ribonucleoproteins (hnRNPs) comprise a large group of modular RNA-binding proteins classified according to their conserved domains. This modular nature, coupled with a large choice of alternative ...

Article

Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells

Antoniades, Ioanna; Stylianou, Panayiota; Christodoulou, Neophytos; Skourides, Paris A. (2017)

We previously identified focal adhesion kinase (FAK) as an important regulator of ciliogenesis in multiciliated cells. FAK and other focal adhesion (FA) proteins associate with the basal bodies and their striated rootlets ...

Article

Autosomal dominant polycystic kidney disease: Molecular genetics and molecular pathogenesis

Koptides, Michael; Constantinou-Deltas, Constantinos D. (2000)

Mutations in three different genes, PKD1, PKD2 and PKD3, can cause a very similar clinical picture of the autosomal dominant form of polycystic kidney disease (ADPKD). Apparently, mutations in the PKD3 gene, which is still ...

Article

Evidence for activation of the unfolded protein response in collagen iv nephropathies

Pieri, Myrtani; Stefanou, Charalambos; Zaravinos, Apostolos; Erguler, K.; Stylianou, Konstantinos G.; Lapathitis, Georgios; Karaiskos, Christos; Savva, Isavella; Paraskeva, Revekka; Dweep, H.; Sticht, C.; Anastasiadou, Natassa; Zouvani, Ioanna; Goumenos, Dimitrios S.; Felekkis, Kyriacos N.; Saleem, M.; Voskarides, Konstantinos; Gretz, N.; Constantinou-Deltas, Constantinos D. (2014)

Thin-basement-membrane nephropathy (TBMN) and Alport syndrome (AS) are progressive collagen IV nephropathies caused by mutations in COL4A3/A4/A5 genes. These nephropathies invariably present with microscopic hematuria and ...

Article

Identification, Distribution and Physiological Activity of Three Novel Neuropeptides of Lymnaea: FLRlamide and pQFYRlamide Encoded by the FMRFamide Gene, and a Related Peptide

Santama, Niovi; Wheeler, C. H.; Skingsley, D. R.; Yeoman, M. S.; Bright, K.; Kaye, I.; Burke, J. F.; Benjamin, P. R. (1995)

We are interested in analysing the detailed modulation of defined neuronal systems by multiple neuropeptides encoded in the FMRFamide locus of the snail Lymnaea. Cloning of the FMRFamide gene has predicted the existence ...

Article

The interplay between DSL proteins and ubiquitin ligases in Notch signaling

Pitsouli, Chrysoula; Delidakis, Christos (2005)

Lateral inhibition is a pattern refining process that generates single neural precursors from a field of equipotent cells and is mediated via Notch signaling. Of the two Notch ligands Delta and Serrate, only the former was ...

Article

KIF1Bβ transports dendritically localized mRNPs in neurons and is recruited to synapses in an activity-dependent manner

Charalambous, Despina C.; Pasciuto, E.; Mercaldo, V.; Pilo Boyl, P.; Munck, S.; Bagni, C.; Santama, Niovi (2013)

KIF1Bβ is a kinesin-like, microtubule-based molecular motor protein involved in anterograde axonal vesicular transport in vertebrate and invertebrate neurons. Certain KIF1Bβ isoforms have been implicated in different forms ...

Article

Neural network controlling feeding in Lymnaea stagnalis: Immunocytochemical localization of myomodulin, small cardioactive peptide, buccalin, and FMRFamide‐ related peptides

Santama, Niovi; Brierley, M.; Burke, J. F.; Benjamin, P. R. (1994)

This paper investigates the distribution of four classes of neuropeptides, myomodulin, small cardioactive peptide (SCP), buccalin, and FMRFamide, in central neurons forming the network that underlies feeding behavior in ...

Article

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis

Kypri, Elena; Christodoulou, A.; Maimaris, G.; Lethan, M.; Markaki, M.; Lysandrou, C.; Lederer, C. W.; Tavernarakis, N.; Geimer, S.; Pedersen, L. B.; Santama, Niovi (2014)

Nucleotide-binding proteins Nubp1 and Nubp2 are MRP/MinD-type P-loop NTPases with sequence similarity to bacterial division site-determining proteins and are conserved, essential proteins throughout the Eukaryotes. They ...

Article

Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins

Skourides, Paris A.; Perera, S. A.; Ren, R. (1999)

Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether ...

Article

The Polycomb Group Protein SUZ12 regulates histone H3 lysine 9 methylation and HP1α distribution

De La Cruz, C. C.; Kirmizis, Antonis; Simon, M. D.; Isono, K. -I; Koseki, H.; Panning, B. (2007)

Regulation of histone methylation is critical for proper gene expression and chromosome function. Suppressor of Zeste 12 (SUZ12) is a requisite member of the EED/EZH2 histone methyltransferase complexes, and is required ...

Article

Processing of the FMRFamide Precursor Protein in the Snail Lymnaea stagnalis: Characterization and Neuronal Localization of a Novel Peptide, ‘SEEPLY’

Santama, Niovi; Wan Li, K.; Bright, K. E.; Yeoman, M.; Geraerts, W. P. M.; Benjamin, P. R.; Burke, J. F. (1993)

In the pulmonate snail Lymnaea stagnalis, FMRFamide‐like neuropeptides are encoded by a multi‐exon genomic locus which is subject to regulation at the level of mRNA splicing. We aim to understand the post‐translational ...

Article

Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27

Kirmizis, Antonis; Bartley, S. M.; Kuzmichev, A.; Margueron, R.; Reinberg, D.; Green, R.; Farnham, P. J. (2004)

Polycomb group (PcG) complexes 2 and 3 are involved in transcriptional silencing. These complexes contain a histone lysine methyltransferase (HKMT) activity that targets different lysine residues on histones H1 or H3 in ...

Article

Spectral density ratio based clustering methods for the binary segmentation of protein sequences: A comparative study

Ioannou, Androulla; Fokianos, Konstantinos; Promponas, Vasilis J. (2010)

We compare several spectral domain based clustering methods for partitioning protein sequence data. The main instrument for this exercise is the spectral density ratio model, which specifies that the logarithmic ratio of ...

Article

Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo

Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2011)

Background: Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within ...

Article

Xenopus laevis nucleotide binding protein 1 (xNubp1) is important for convergent extension movements and controls ciliogenesis via regulation of the actin cytoskeleton

Ioannou, Androulla; Santama, Niovi; Skourides, Paris A. (2013)

Nucleotide binding protein 1 (Nubp1) is a highly conserved phosphate loop (P-loop) ATPase involved in diverse processes including iron-sulfur protein assembly, centrosome duplication and lung development. Here, we report ...