dc.contributor.author | Gatica, Joao | en |
dc.contributor.author | Tripathi, Vijay | en |
dc.contributor.author | Green, Stefan | en |
dc.contributor.author | Manaia, C. M. | en |
dc.contributor.author | Berendonk, Thomas U. | en |
dc.contributor.author | Cacace, Damiano | en |
dc.contributor.author | Merlin, Christophe | en |
dc.contributor.author | Kreuzinger, Norbert | en |
dc.contributor.author | Schwartz, Thomas | en |
dc.contributor.author | Fatta-Kassinos, Despo | en |
dc.contributor.author | Rizzo, Luigi | en |
dc.contributor.author | Schwermer, Carsten U. | en |
dc.contributor.author | Garelick, Hemda | en |
dc.contributor.author | Jurkevitch, Edouard | en |
dc.contributor.author | Cytryn, Eddie | en |
dc.creator | Gatica, Joao | en |
dc.creator | Tripathi, Vijay | en |
dc.creator | Green, Stefan | en |
dc.creator | Manaia, C. M. | en |
dc.creator | Berendonk, Thomas U. | en |
dc.creator | Cacace, Damiano | en |
dc.creator | Merlin, Christophe | en |
dc.creator | Kreuzinger, Norbert | en |
dc.creator | Schwartz, Thomas | en |
dc.creator | Fatta-Kassinos, Despo | en |
dc.creator | Rizzo, Luigi | en |
dc.creator | Schwermer, Carsten U. | en |
dc.creator | Garelick, Hemda | en |
dc.creator | Jurkevitch, Edouard | en |
dc.creator | Cytryn, Eddie | en |
dc.date.accessioned | 2019-04-18T06:19:06Z | |
dc.date.available | 2019-04-18T06:19:06Z | |
dc.date.issued | 2016 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/45486 | |
dc.description.abstract | Integrons are extensively targeted as a proxy for anthropogenic impact in the environment. We developed a novel high-throughput amplicon sequencing pipeline that enables characterization of thousands of integron gene cassette-associated reads, and applied it to acquire a comprehensive overview of gene cassette composition in effluents from wastewater treatment facilities across Europe. Between 38 100 and 172 995 reads per-sample were generated and functionally characterized by screening against nr, SEED, ARDB and &beta | en |
dc.description.abstract | -lactamase databases. Over 75% of the reads were characterized as hypothetical, but thousands were associated with toxin-antitoxin systems, DNA repair, cell membrane function, detoxification and aminoglycoside and &beta | en |
dc.description.abstract | -lactam resistance. Among the reads characterized as &beta | en |
dc.description.abstract | -lactamases, the carbapenemase bla^sub OXA^ was dominant in most of the effluents, except for Cyprus and Israel where blaGES was also abundant. Quantitative PCR assessment of bla^sub OXA^ and bla^sub GES^ genes in the European effluents revealed similar trends to those displayed in the integron amplicon sequencing pipeline described above, corroborating the robustness of this method and suggesting that these integron-associated genes may be excellent targets for source tracking of effluents in downstream environments. Further application of the above analyses revealed several order-of-magnitude reductions in effluent-associated &beta | en |
dc.description.abstract | -lactamase genes in effluent-saturated soils, suggesting marginal persistence in the soil microbiome. | en |
dc.language.iso | eng | en |
dc.source | Environmental science & technology | en |
dc.source.uri | http://search.proquest.com/docview/1836582134?accountid=17200 | |
dc.subject | Polymerase chain reaction | en |
dc.subject | Membranes | en |
dc.subject | Microbiology | en |
dc.subject | Environmental Studies | en |
dc.subject | Water treatment | en |
dc.subject | Effluents | en |
dc.subject | Genetics | en |
dc.subject | Toxins | en |
dc.title | High Throughput Analysis of Integron Gene Cassettes in Wastewater Environments | en |
dc.type | info:eu-repo/semantics/article | |
dc.description.volume | 50 | |
dc.description.issue | 21 | |
dc.author.faculty | Πολυτεχνική Σχολή / Faculty of Engineering | |
dc.author.department | Τμήμα Πολιτικών Μηχανικών και Μηχανικών Περιβάλλοντος / Department of Civil and Environmental Engineering | |
dc.type.uhtype | Article | en |
dc.contributor.orcid | Fatta-Kassinos, Despo [0000-0003-1173-0941] | |
dc.gnosis.orcid | 0000-0003-1173-0941 | |