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dc.contributor.authorGatica, Joaoen
dc.contributor.authorTripathi, Vijayen
dc.contributor.authorGreen, Stefanen
dc.contributor.authorManaia, C. M.en
dc.contributor.authorBerendonk, Thomas U.en
dc.contributor.authorCacace, Damianoen
dc.contributor.authorMerlin, Christopheen
dc.contributor.authorKreuzinger, Norberten
dc.contributor.authorSchwartz, Thomasen
dc.contributor.authorFatta-Kassinos, Despoen
dc.contributor.authorRizzo, Luigien
dc.contributor.authorSchwermer, Carsten U.en
dc.contributor.authorGarelick, Hemdaen
dc.contributor.authorJurkevitch, Edouarden
dc.contributor.authorCytryn, Eddieen
dc.creatorGatica, Joaoen
dc.creatorTripathi, Vijayen
dc.creatorGreen, Stefanen
dc.creatorManaia, C. M.en
dc.creatorBerendonk, Thomas U.en
dc.creatorCacace, Damianoen
dc.creatorMerlin, Christopheen
dc.creatorKreuzinger, Norberten
dc.creatorSchwartz, Thomasen
dc.creatorFatta-Kassinos, Despoen
dc.creatorRizzo, Luigien
dc.creatorSchwermer, Carsten U.en
dc.creatorGarelick, Hemdaen
dc.creatorJurkevitch, Edouarden
dc.creatorCytryn, Eddieen
dc.date.accessioned2019-04-18T06:19:06Z
dc.date.available2019-04-18T06:19:06Z
dc.date.issued2016
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/45486
dc.description.abstractIntegrons are extensively targeted as a proxy for anthropogenic impact in the environment. We developed a novel high-throughput amplicon sequencing pipeline that enables characterization of thousands of integron gene cassette-associated reads, and applied it to acquire a comprehensive overview of gene cassette composition in effluents from wastewater treatment facilities across Europe. Between 38 100 and 172 995 reads per-sample were generated and functionally characterized by screening against nr, SEED, ARDB and &betaen
dc.description.abstract-lactamase databases. Over 75% of the reads were characterized as hypothetical, but thousands were associated with toxin-antitoxin systems, DNA repair, cell membrane function, detoxification and aminoglycoside and &betaen
dc.description.abstract-lactam resistance. Among the reads characterized as &betaen
dc.description.abstract-lactamases, the carbapenemase bla^sub OXA^ was dominant in most of the effluents, except for Cyprus and Israel where blaGES was also abundant. Quantitative PCR assessment of bla^sub OXA^ and bla^sub GES^ genes in the European effluents revealed similar trends to those displayed in the integron amplicon sequencing pipeline described above, corroborating the robustness of this method and suggesting that these integron-associated genes may be excellent targets for source tracking of effluents in downstream environments. Further application of the above analyses revealed several order-of-magnitude reductions in effluent-associated &betaen
dc.description.abstract-lactamase genes in effluent-saturated soils, suggesting marginal persistence in the soil microbiome.en
dc.language.isoengen
dc.sourceEnvironmental science & technologyen
dc.source.urihttp://search.proquest.com/docview/1836582134?accountid=17200
dc.subjectPolymerase chain reactionen
dc.subjectMembranesen
dc.subjectMicrobiologyen
dc.subjectEnvironmental Studiesen
dc.subjectWater treatmenten
dc.subjectEffluentsen
dc.subjectGeneticsen
dc.subjectToxinsen
dc.titleHigh Throughput Analysis of Integron Gene Cassettes in Wastewater Environmentsen
dc.typeinfo:eu-repo/semantics/article
dc.description.volume50
dc.description.issue21
dc.author.facultyΠολυτεχνική Σχολή / Faculty of Engineering
dc.author.departmentΤμήμα Πολιτικών Μηχανικών και Μηχανικών Περιβάλλοντος / Department of Civil and Environmental Engineering
dc.type.uhtypeArticleen
dc.contributor.orcidFatta-Kassinos, Despo [0000-0003-1173-0941]
dc.gnosis.orcid0000-0003-1173-0941


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