dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Vaughan, A. T. M. | en |
dc.contributor.author | Yamasaki, H. | en |
dc.contributor.author | Kamath, N. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Vaughan, A. T. M. | en |
dc.creator | Yamasaki, H. | en |
dc.creator | Kamath, N. | en |
dc.date.accessioned | 2019-11-04T12:50:24Z | |
dc.date.available | 2019-11-04T12:50:24Z | |
dc.date.issued | 1996 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53005 | |
dc.description.abstract | To explore the program of cell differentiation in Friend murine erythroleukemia (MEL) cells, we used three clonal variants: phorbol 12- myristate 13-acetate (PMA)-hypersensitive TS-19-101, PMA-resistant TR19-9, and hexamethylene bis-acetamide (HMBA)- and PMA-resistant DS19/R1. After treating TS19-101 cells with HMBA, topoisomerase II (topo II) enzymatic activity was dramatically reduced, and cells became terminally differentiated. The initial reduction in activity was soon followed by reduced topo IIα phosphorylation, but only later did the protein level drop significantly. PMA, which completely blocked HMBA-induced differentiation in TS19-101 cells, increased the phosphorylation of topo IIα and restored the enzymatic activity to its original levels. Reduced topo II activity and phosphorylation were also evident in HMBA-treated TR19-9 cells. PMA failed to restore topo II activity and phosphorylation to their original levels in TR19-9 cells. Predictably, the topo II activity and phosphorylation of DS19/R1 cells showed little change in response to HMBA or PMA treatment. Structural changes in chromatin became evident in sensitive cells 24 h after HMBA treatment, suggesting that alterations in topo IIα phosphorylation may control cell differentiation by altering nuclear architecture. | en |
dc.source | Cancer research | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029789457&partnerID=40&md5=c5e97531d33030aeb459435efd5b0d26 | |
dc.subject | article | en |
dc.subject | Antineoplastic Agents | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | nonhuman | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | animal cell | en |
dc.subject | mouse | en |
dc.subject | DNA-Binding Proteins | en |
dc.subject | cell differentiation | en |
dc.subject | Kinetics | en |
dc.subject | enzyme activity | en |
dc.subject | enzyme phosphorylation | en |
dc.subject | Acetamides | en |
dc.subject | Cell Nucleus | en |
dc.subject | Models, Biological | en |
dc.subject | Cell Survival | en |
dc.subject | Antigens, Neoplasm | en |
dc.subject | DNA Topoisomerases, Type II | en |
dc.subject | DNA Topoisomerases, Type II, Eukaryotic | en |
dc.subject | Isoenzymes | en |
dc.subject | Phosphorylation | en |
dc.subject | DNA Topoisomerases, Type I | en |
dc.subject | dna topoisomerase (atp hydrolysing) | en |
dc.subject | Tetradecanoylphorbol Acetate | en |
dc.subject | Chromatin | en |
dc.subject | chromatin structure | en |
dc.subject | Clone Cells | en |
dc.subject | erythroleukemia cell | en |
dc.subject | Friend murine leukemia virus | en |
dc.subject | hexamethylenebisacetamide | en |
dc.subject | Leukemia, Experimental | en |
dc.subject | phorbol 12 acetate 13 myristate | en |
dc.title | Commitment to erythroid differentiation in mouse erythroleukemia cells is controlled by alterations in topoisomerase IIα phosphorylation | en |
dc.type | info:eu-repo/semantics/article | |
dc.description.volume | 56 | |
dc.description.startingpage | 4192 | |
dc.description.endingpage | 4199 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :16</p> | en |
dc.source.abbreviation | Cancer Res. | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 | |