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Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms

dc.contributor.authorLiu, J.en
dc.contributor.authorBurdette, J. E.en
dc.contributor.authorXu, H.en
dc.contributor.authorGu, C.en
dc.contributor.authorVan Breemen, R. B.en
dc.contributor.authorBhat, K. P. L.en
dc.contributor.authorBooth, N.en
dc.contributor.authorConstantinou, Andreas I.en
dc.contributor.authorPezzuto, J. M.en
dc.contributor.authorFong, H. H. S.en
dc.contributor.authorFarnsworth, N. R.en
dc.contributor.authorBolton, J. L.en
dc.creatorLiu, J.en
dc.creatorBurdette, J. E.en
dc.creatorXu, H.en
dc.creatorGu, C.en
dc.creatorVan Breemen, R. B.en
dc.creatorBhat, K. P. L.en
dc.creatorBooth, N.en
dc.creatorConstantinou, Andreas I.en
dc.creatorPezzuto, J. M.en
dc.creatorFong, H. H. S.en
dc.creatorFarnsworth, N. R.en
dc.creatorBolton, J. L.en
dc.date.accessioned2019-11-04T12:52:17Z
dc.date.available2019-11-04T12:52:17Z
dc.date.issued2001
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53229
dc.description.abstractEight botanical preparations that are commonly used for the treatment of menopausal symptoms were tested for estrogenic activity. Methanol extracts of red clover (Trifolium pratense L.), chasteberry (Vitex agnus-castus L.), and hops (Humulus lupulus L.) showed significant competitive binding to estrogen receptors α (ERα) and β (ERβ). With cultured Ishikawa (endometrial) cells, red clover and hops exhibited estrogenic activity as indicated by induction of alkaline phosphatase (AP) activity and up-regulation of progesterone receptor (PR) mRNA. Chasteberry also stimulated PR expression, but no induction of AP activity was observed. In S30 breast cancer cells, pS2 (pres enelin-2), another estrogen-inducible gene, was up-regulated in the presence of red clover, hops, and chasteberry. Interestingly, extracts of Asian ginseng (Panax ginseng C.A. Meyer) and North American ginseng (Panax quinquefolius L.) induced pS2 mRNA expression in S30 cells, but no significant ER binding affinity, AP induction, or PR expression was noted in Ishikawa cells. Dong quai [Angelica sinensis (Oliv.) Diels] and licorice (Glycyrrhiza glabra L.) showed only weak ER binding and PR and pS2 mRNA induction. Black cohosh [Cimicifuga racemosa (L.) Nutt.] showed no activity in any of the above in vitro assays. Bioassay-guided isolation utilizing ER competitive binding as a monitor and screening using ultrafiltration LC-MS revealed that genistein was the most active component of red clover. Consistent with this observation, genistein was found to be the most effective of four red clover isoflavones tested in the above in vitro assays. Therefore, estrogenic components of plant extracts can be identified using assays for estrogenic activity along with screening and identification of the active components using ultrafiltration LC-MS. These data suggest a potential use for some dietary supplements, ingested by human beings, in the treatment of menopausal symptoms.en
dc.sourceJournal of Agricultural and Food Chemistryen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-17944378660&doi=10.1021%2fjf0014157&partnerID=40&md5=24133299f6dcbe4b7120cab14c28adfd
dc.subjectarticleen
dc.subjecthumanen
dc.subjectHumansen
dc.subjectbreast canceren
dc.subjectcontrolled studyen
dc.subjectdrug effecten
dc.subjectmetabolismen
dc.subjectdrug screeningen
dc.subjecthuman cellen
dc.subjectdiet supplementationen
dc.subjectphysiologyen
dc.subjectmessenger RNAen
dc.subjectestrogen activityen
dc.subjectestrogenen
dc.subjectmenopauseen
dc.subjectpS2en
dc.subjectchemistryen
dc.subjectplant extracten
dc.subjectcancer cell cultureen
dc.subjectUp-Regulationen
dc.subjectEstrogen receptoren
dc.subjectProgesterone receptoren
dc.subjectreceptor upregulationen
dc.subjectEstrogensen
dc.subjectcell cultureen
dc.subjectgenisteinen
dc.subjectCimicifuga racemosa extracten
dc.subjectPlant Extractsen
dc.subjectReceptors, Estrogenen
dc.subjectisoflavone derivativeen
dc.subjectIsoflavonesen
dc.subjectTrifolium pratenseen
dc.subjectPhytoestrogensen
dc.subjectEstrogens, Non-Steroidalen
dc.subjectDietary Supplementsen
dc.subjectCells, Cultureden
dc.subjectAlkaline phosphataseen
dc.subjectAngelica sinensis extracten
dc.subjectbinding competitionen
dc.subjectBinding, Competitiveen
dc.subjectDietary supplementen
dc.subjectdrug isolationen
dc.subjectdrug receptor bindingen
dc.subjectendometrium cellen
dc.subjectEstrogens, Non Steroidalen
dc.subjectginseng extracten
dc.subjectGlycyrrhiza extracten
dc.subjecthigher planten
dc.subjectHumulus lupulus extracten
dc.subjectpanax quinquefoliusen
dc.subjectPlant Preparationsen
dc.subjectpresenilin 2en
dc.subjectReceptors, Progesteroneen
dc.subjectVitex agnus castusen
dc.titleEvaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptomsen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1021/jf0014157
dc.description.volume49
dc.description.startingpage2472
dc.description.endingpage2479
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :327</p>en
dc.source.abbreviationJ.Agric.Food Chem.en
dc.contributor.orcidConstantinou, Andreas I. [0000-0003-0365-1821]
dc.gnosis.orcid0000-0003-0365-1821


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