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dc.contributor.authorMuro, E.en
dc.contributor.authorVermeulen, P.en
dc.contributor.authorIoannou, Androullaen
dc.contributor.authorSkourides, Paris A.en
dc.contributor.authorDubertret, B.en
dc.contributor.authorFragola, A.en
dc.contributor.authorLoriette, V.en
dc.creatorMuro, E.en
dc.creatorVermeulen, P.en
dc.creatorIoannou, Androullaen
dc.creatorSkourides, Paris A.en
dc.creatorDubertret, B.en
dc.creatorFragola, A.en
dc.creatorLoriette, V.en
dc.date.accessioned2019-11-04T12:52:23Z
dc.date.available2019-11-04T12:52:23Z
dc.date.issued2011
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53263
dc.description.abstractWe describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving sample which is illuminated simultaneously with a flat and a structured pattern at two different wavelengths. Both homogenous and structured fluorescence images are spectrally separated at detection and combined similarly with the HiLo microscopy technique. We present optically sectioned full-field images of Xenopus laevis embryos acquired at 25 images/s frame rate. © 2011 by the Biophysical Society.en
dc.sourceBiophysical journalen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79960295038&doi=10.1016%2fj.bpj.2011.03.047&partnerID=40&md5=7f772a2691129eb8e3329bd082411141
dc.subjectmethodologyen
dc.subjectarticleen
dc.subjectAnimalsen
dc.subjectanimalen
dc.subjectchemistryen
dc.subjectinstrumentationen
dc.subjectlight related phenomenaen
dc.subjectXenopus laevisen
dc.subjectprenatal developmenten
dc.subjectfluorescent dyeen
dc.subjectFluorescent Dyesen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectcoloren
dc.subjectfluorescence microscopyen
dc.subjectmicrosphereen
dc.subjectMicrospheresen
dc.subjectOptical Processesen
dc.titleSingle-shot optical sectioning using two-color probes in HiLo fluorescence microscopyen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1016/j.bpj.2011.03.047
dc.description.volume100
dc.description.startingpage2810
dc.description.endingpage2819
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :4</p>en
dc.source.abbreviationBiophys.J.en
dc.contributor.orcidSkourides, Paris A. [0000-0003-3502-5729]


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