dc.contributor.author | Santama, Niovi | en |
dc.contributor.author | Krijnse-Ĺocker, J. | en |
dc.contributor.author | Griffiths, G. | en |
dc.contributor.author | Noda, Y. | en |
dc.contributor.author | Hirokawa, N. | en |
dc.contributor.author | Dotti, C. G. | en |
dc.creator | Santama, Niovi | en |
dc.creator | Krijnse-Ĺocker, J. | en |
dc.creator | Griffiths, G. | en |
dc.creator | Noda, Y. | en |
dc.creator | Hirokawa, N. | en |
dc.creator | Dotti, C. G. | en |
dc.date.accessioned | 2019-11-04T12:52:36Z | |
dc.date.available | 2019-11-04T12:52:36Z | |
dc.date.issued | 1998 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53359 | |
dc.description.abstract | Lysosomes concentrate juxtanuclearly in the region around the microtubule-organizing center by interaction with microtubules. Different experimental and physiological conditions can induce these organelles to move to the cell periphery by a mechanism implying a plus-end-directed microtubule-motor protein (a kinesin-like motor). The responsible kinesin-superfamily protein, however, is unknown. We have identified a new mouse isoform of the kinesin superfamily, KIF2β, an alternatively spliced isoform of the known, neuronal kinesin, KIF2. Developmental expression pattern and cell-type analysis in vivo and in vitro reveal that KIF2β is abundant at early developmental stages of the hippocampus but is then downregulated in differentiated neuronal cells, and it is mainly or uniquely expressed in non-neuronal cells while KIF2 remains exclusively neuronal. Electron microscopy of mouse fibroblasts and immunofluorescence of KIF2β-transiently-transfected fibroblasts show KIF2 and KIF2β primarily associated with lysosomes, and this association can be disrupted by detergent treatment. In KIF2β-overexpressing cells, lysosomes (labeled with anti-lysosome-associated membrane protein-1) become abnormally large and peripherally located at some distance from their usual perinuclear positions. Overexpression of KIF2 or KIF2β does not change the size or distribution of early, late and recycling endosomes nor does overexpression of different kinesin superfamily proteins result in changes in lysosome size or positioning. These results implicate KIF2β as a motor responsible for the peripheral translocation of lysosomes. | en |
dc.source | EMBO Journal | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032531562&doi=10.1093%2femboj%2f17.20.5855&partnerID=40&md5=c0ecab3aefbb8a88e27a577076d915b9 | |
dc.subject | article | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | amino acid sequence | en |
dc.subject | immunohistochemistry | en |
dc.subject | protein expression | en |
dc.subject | gene expression regulation | en |
dc.subject | nonhuman | en |
dc.subject | pathology | en |
dc.subject | newborn | en |
dc.subject | immunofluorescence | en |
dc.subject | metabolism | en |
dc.subject | electron microscopy | en |
dc.subject | molecular genetics | en |
dc.subject | Animals | en |
dc.subject | Mice | en |
dc.subject | animal | en |
dc.subject | animal cell | en |
dc.subject | biosynthesis | en |
dc.subject | mouse | en |
dc.subject | genetics | en |
dc.subject | physiology | en |
dc.subject | Base Sequence | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | cell differentiation | en |
dc.subject | Lysosomes | en |
dc.subject | membrane protein | en |
dc.subject | nucleotide sequence | en |
dc.subject | isolation and purification | en |
dc.subject | DNA sequence | en |
dc.subject | enzymology | en |
dc.subject | Neurons | en |
dc.subject | Fibroblasts | en |
dc.subject | fibroblast | en |
dc.subject | embryo | en |
dc.subject | cell culture | en |
dc.subject | genetic transfection | en |
dc.subject | Sequence Analysis, DNA | en |
dc.subject | Isoenzymes | en |
dc.subject | Gene Expression Regulation, Developmental | en |
dc.subject | nerve cell | en |
dc.subject | protein family | en |
dc.subject | nervous system development | en |
dc.subject | rat | en |
dc.subject | protein transport | en |
dc.subject | Cells, Cultured | en |
dc.subject | complementary DNA | en |
dc.subject | DNA, Complementary | en |
dc.subject | messenger rna | en |
dc.subject | isoenzyme | en |
dc.subject | Motor proteins | en |
dc.subject | 3T3 Cells | en |
dc.subject | alternative rna splicing | en |
dc.subject | cell nucleus | en |
dc.subject | cell strain 3T3 | en |
dc.subject | centrifugation | en |
dc.subject | detergent | en |
dc.subject | endosome | en |
dc.subject | hippocampus | en |
dc.subject | intracellular fluid | en |
dc.subject | Kif2b protein, mouse | en |
dc.subject | kinesin | en |
dc.subject | Kinesins | en |
dc.subject | Lamp-1,2 | en |
dc.subject | lysosome | en |
dc.subject | Microtubules | en |
dc.title | KIF2β, a new kinesin superfamily protein in non-neuronal cells, is associated with lysosomes and may be implicated in their centrifugal translocation | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1093/emboj/17.20.5855 | |
dc.description.volume | 17 | |
dc.description.startingpage | 5855 | |
dc.description.endingpage | 5867 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :50</p> | en |
dc.source.abbreviation | EMBO J. | en |
dc.contributor.orcid | Santama, Niovi [0000-0002-8370-8674] | |
dc.gnosis.orcid | 0000-0002-8370-8674 | |