dc.contributor.author | Valentin, A. | en |
dc.contributor.author | Trivedi, H. | en |
dc.contributor.author | Lu, W. | en |
dc.contributor.author | Kostrikis, Leontios G. | en |
dc.contributor.author | Pavlakis, G. N. | en |
dc.creator | Valentin, A. | en |
dc.creator | Trivedi, H. | en |
dc.creator | Lu, W. | en |
dc.creator | Kostrikis, Leontios G. | en |
dc.creator | Pavlakis, G. N. | en |
dc.date.accessioned | 2019-11-04T12:52:47Z | |
dc.date.available | 2019-11-04T12:52:47Z | |
dc.date.issued | 2000 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53427 | |
dc.description.abstract | CCR5 and CXCR4 are the main coreceptors for non-syncytia-inducing (NSI) and syncytia-inducing (SI) HIV-1 strains, respectively. NSI HIV-1 isolates do not infect either human lymphoid or monocytoid cell lines, and this inability correlates with the absence of CCR5 expression in these cell types. The ability of SI HIV-1 isolates to infect human primary macrophages has been disputed. Here, we report that CXCR4 is expressed in primary blood-derived human mononuclear phagocytes at all stages of differentiation, although the maturation process correlates with downregulation of CXCR4 mRNA. Infection experiments with the SI molecular clone NL4-3 tagged with a mutant of the green fluorescent protein established that both monocytes and attached macrophages are susceptible to infection with CXCR4-restricted HIV-1 strains. NL4-3 entry into primary macrophages could be blocked by SDF-1α in a dose- dependent manner, or by the anti-CXCR4 monoclonal antibody 12G5. HIV-1 entry led to productive infection. No evidence of postentry defects or nuclear import delay for CXCR4-restricted HIV-1 strains was detected using a quantitative real-time PCR assay measuring HIV-1 DNA entry into the nucleus. Macrophages infected by HIV-1 and expressing virus were maintained in culture for long periods of time (up to 5 months). These results demonstrate that CXCR4 is the main HIV-1 SI coreceptor in human primary macrophages and underline the importance of the macrophage as a long-living viral reservoir for HIV-1. (C) 2000 Academic Press. | en |
dc.source | Virology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034630165&doi=10.1006%2fviro.1999.0136&partnerID=40&md5=9b5be812687554f1dd96c17df4d5aae1 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | priority journal | en |
dc.subject | Human immunodeficiency virus infection | en |
dc.subject | protein analysis | en |
dc.subject | protein expression | en |
dc.subject | monoclonal antibody | en |
dc.subject | Human immunodeficiency virus | en |
dc.subject | down regulation | en |
dc.subject | human cell | en |
dc.subject | monocyte | en |
dc.subject | T-Lymphocytes | en |
dc.subject | internalization | en |
dc.subject | polymerase chain reaction | en |
dc.subject | cell differentiation | en |
dc.subject | Human immunodeficiency virus 1 | en |
dc.subject | HIV-1 | en |
dc.subject | Macrophages | en |
dc.subject | chemokine receptor CXCR4 | en |
dc.subject | macrophage | en |
dc.subject | virus pathogenesis | en |
dc.subject | Cloning, Molecular | en |
dc.subject | cell maturation | en |
dc.subject | Cells, Cultured | en |
dc.subject | DNA determination | en |
dc.subject | Flow Cytometry | en |
dc.subject | RNA viruses | en |
dc.subject | Ribonucleases | en |
dc.subject | Receptors, CXCR4 | en |
dc.subject | hiv 1 | en |
dc.subject | mononuclear phagocyte | en |
dc.title | CXCR4 mediates entry and productive infection of syncytia-inducing (x4) hiv-1 strains in primary macrophages | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1006/viro.1999.0136 | |
dc.description.volume | 269 | |
dc.description.startingpage | 294 | |
dc.description.endingpage | 304 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :39</p> | en |
dc.source.abbreviation | Virology | en |
dc.contributor.orcid | Kostrikis, Leontios G. [0000-0002-5340-7109] | |
dc.gnosis.orcid | 0000-0002-5340-7109 | |