UV resonance Raman study of TTR(105-115) structural evolution as a function of temperature
AuthorPieridou, Galatia K.
Archontis, Georgios Z.
Hayes, Sophia C.
SourceJournal of Physical Chemistry B
Google Scholar check
MetadataShow full item record
UV resonance Raman spectroscopy was used to probe the temperature dependence of the conformation of TTR(105-115) in solution. Resonance Raman spectra with excitation at 239.5 nm, show an increase in the absolute resonance Raman cross section of Tyr with an increase in temperature. This trend is associated with an increase in the hydrophobicity of the Tyr local environment, suggesting a conformational change at 28 °C. Excitation at ∼200 nm is known to enhance scattering due to amide vibrations and provides insights as to the secondary structure of a peptide or protein. UVRR spectra at this excitation suggest that in solution the peptide assumes a disordered conformation with frequent formation of β-turns. Explicit-solvent replica-exchange MD simulations of the isolated peptide in the region 15 to 37 °C suggest that the dominant conformation assumed by the peptide corresponds to a coil with β-turns in the central and C-terminal region. In line with the experiments, an increase in temperature induces structural order in the peptide, reflected by an increase in the probability for the formation of β-turns and hydrophobic side-chain contacts, mainly in the 8-11 moiety, and to a lesser extent in the 4-7 moiety. © 2011 American Chemical Society.
Showing items related by title, author, creator and subject.
Pieridou, Galatia K.; Avgousti-Menelaou, C.; Tamamis, Phanourios; Archontis, Georgios Z.; Hayes, Sophia C. (2011)UV resonance Raman spectroscopy was used to probe the temperature dependence of the conformation of TTR(105-115) in solution. Resonance Raman spectra with excitation at 239.5 nm, show an increase in the absolute resonance ...
Trimithioti, Marilena; Hayes, Sophia C. (2013)Halogens such as chlorine are converted from halides, including ClNO 2, to reactive radicals by UV solar radiation. These radicals can affect ozone production and destruction in the stratosphere. Recently, it became clear ...
Simultaneous resonance Raman detection of the heme a3-Fe-CO and CuB-CO species in CO-bound ba3-cytochrome c oxidase from Thermus thermophilus: Evidence for a charge transfer CuB-CO transition Pinakoulaki, Eftychia; Ohta, T.; Soulimane, T.; Kitagawa, T.; Varotsis, Constantinos (2004)Understanding of the chemical nature of the dioxygen and nitric oxide moiety of ba3-cytochrome c oxidase from Thermus thermophilus is crucial for elucidation of its physiological function. In the present work, direct ...