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dc.contributor.authorMitrelias, Thanosen
dc.contributor.authorTrypiniotis, Theodossisen
dc.contributor.authorPalfreyman, J. J.en
dc.contributor.authorHong, B.en
dc.contributor.authorVyas, K.en
dc.contributor.authorHayward, T. J.en
dc.contributor.authorLlandro, J.en
dc.contributor.authorKopper, K. P.en
dc.contributor.authorBland, J. A. C.en
dc.contributor.authorRobertson, P. A.en
dc.contributor.authorBarnes, C. H. W.en
dc.creatorMitrelias, Thanosen
dc.creatorTrypiniotis, Theodossisen
dc.creatorPalfreyman, J. J.en
dc.creatorHong, B.en
dc.creatorVyas, K.en
dc.creatorHayward, T. J.en
dc.creatorLlandro, J.en
dc.creatorKopper, K. P.en
dc.creatorBland, J. A. C.en
dc.creatorRobertson, P. A.en
dc.creatorBarnes, C. H. W.en
dc.description.abstractMicroarrays and suspension (or bead)-based technologies have attracted significant interest for their broad applications in high throughput molecular biology. However, the throughput of microarrays will always be limited by the array density and the slow diffusion of molecules to their binding sites. Suspension-based technologies, in which all the reactions take place directly on the surface of microcarriers functionalized with molecular probes, could offer true multiplexing due to the possibility of extending their detection capability by a straightforward expansion of the size of the chemical library of probes. To fully exploit their potential, the microcarriers must be tagged, but the number of distinct codes available from spectrometric/graphical/physical encoding methods is currently fairly limited. A digital magnetic tagging method based on magnetic microtags, which have been anisotropy engineered to provide stable magnetization directions which correspond to digital codes, is reported. The tags can be suspended in solution and functionalized with a variety of biological molecular probes. Magnetic tagging offers several benefits compared to the traditional optical encoding techniques currently employed. It offers minimal background signals, potential for a large number of distinct codes, miniaturization of devices, and the ability to write a code in situ. Experimental data showing the reading of individual magnetic microbars from samples comprising 50×20 µ m2 Ni elements, as well as micromagnetic simulations that show the feasibility of stray field detection, are presented. The stray fields of the magnetic microbars spanning a range of 60 mOe were detected by a microfabricated fluxgate sensor scanned in a raster fashion over the sample that was placed about 70 µm away. Free floating tags have also been fabricated for use in microfluidic systems. A magnetic lab-on-a-chip device could be used for tagging biomolecular probes for applications in genome sequencing, immunoassays, clinical diagnostics, drug discovery, and general pathogen detection and screening. © 2009 American Institute of Physics.en
dc.sourceJournal of Applied Physicsen
dc.subjectMolecular biologyen
dc.subjectEncoding (symbols)en
dc.subjectMagnetic materialsen
dc.subjectMagnetic anisotropyen
dc.subjectBinding energyen
dc.subjectMolecular probesen
dc.subjectBinding sitesen
dc.subjectExperimental datumen
dc.subjectOptical data processingen
dc.subjectMagnetic taggingen
dc.subjectArray densitiesen
dc.subjectBackground signalsen
dc.subjectBiochemical assaysen
dc.subjectBroad applicationsen
dc.subjectChemical librariesen
dc.subjectClinical diagnosticsen
dc.subjectDetection capabilitiesen
dc.subjectDigital codesen
dc.subjectDrug discoveriesen
dc.subjectEncoding methodsen
dc.subjectFlux-gate sensorsen
dc.subjectGenome sequencingen
dc.subjectHigh throughputsen
dc.subjectLab-on-a-chip devicesen
dc.subjectMagnetic microbarsen
dc.subjectMagnetization directionsen
dc.subjectMicro fluidic systemsen
dc.subjectMicro-magnetic simulationsen
dc.subjectMiniaturization of devicesen
dc.subjectOptical encodingen
dc.subjectPathogen detectionsen
dc.subjectSlow diffusionsen
dc.subjectStray fieldsen
dc.titleDigital magnetic tagging for multiplexed suspension-based biochemical assaysen
dc.description.issue7Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied SciencesΤμήμα Φυσικής / Department of Physics

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