Browsing by Author "Charalambous, Anna"
Now showing items 1-9 of 9
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Article
1,2,3-Dithiazoles – new reversible melanin synthesis inhibitors: a chemical genomics study
Charalambous, Anna; Koyioni, Maria G.; Antoniades, Ioanna A.; Pegeioti, Despoina; Eleftheriou, Iro; Michaelidou, Sophia S.; Amelichev, Stanislav A.; Konstantinova, Lidia S.; Rakitin, Oleg A.; Koutentis, Panayiotis Andreas; Skourides, Paris A. (2015)
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Article
1,2,3-Dithiazoles-new reversible melanin synthesis inhibitors: A chemical genomics study
Charalambous, Anna; Koyioni, Maria; Antoniades, Ioanna; Pegeioti, Despoina; Eleftheriou, Iro; Michaelidou, Sophia S.; Amelichev, Stanislav A.; Konstantinova, L. S.; Rakitin, O. A.; Koutentis, Panayiotis Andreas; Skourides, Paris A. (2015)A chemical genomic screen of an in-house library of small molecule heterocycles was carried out using Xenopus laevis embryos. This led to the identification of N-(4-chloro-5H-1,2,3-dithiazol-5-ylidene)-4-methoxyaniline ...
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Book Chapter
Determining Temporal and Spatial Expression of Calpains in Amphibians
Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Zanardelli, Sara; Skourides, Paris A. (Springer, 2019)Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that Calpains have been identified in the Xenopus genome, ...
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Article
High-resolution whole-mount in situ hybridization using quantum dot nanocrystals
Ioannou, Androulla; Eleftheriou, Iro; Lubatti, Andrea; Charalambous, Anna; Skourides, Paris A. (2012)The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically ...
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Article
In vivo, site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing
Charalambous, Anna; Andreou, Maria I.; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2012)The ability to target proteins with nanostructures and/or nanodevices in vivo is important for understanding and controlling their biological function. Quantum dots (QDs) serve as an ideal model nanostructure due to their ...
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Article
Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo
Charalambous, Anna; Andreou, Maria I.; Skourides, Paris A. (2009)We describe an intein based method to site-specifically conjugate Quantum Dots (QDs) to target proteins in vivo. This approach allows the covalent conjugation of any nanostructure and/or nanodevice to any protein and thus ...
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Book Chapter
Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
Antoniades, Ioanna; Charalambous, Anna; Christodoulou, Neophytos; Zanardelli, Sara; Skourides, Paris A. (Springer, 2019)Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that the role of calpains in cell migration and other ...
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Book Chapter
Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus
Ioannou, Androulla; Eleftheriou, Iro; Charalambous, Anna; Skourides, Paris A. (Springer New York, 2015)The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount ...
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Article
Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2011)Background: Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within ...