Determining Temporal and Spatial Expression of Calpains in Amphibians
Date
2019Author
Charalambous, AnnaAntoniades, Ioanna
Christodoulou, Neophytos
Zanardelli, Sara
![ORCID logo](https://orcid.org/sites/default/files/images/orcid_16x16.png)
ISBN
978-1-4939-8988-1Publisher
SpringerPlace of publication
New York, NYSource
Calpain: Methods and ProtocolsPages
67-79Google Scholar check
Metadata
Show full item recordAbstract
Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that Calpains have been identified in the Xenopus genome, their expression patterns and role have not been characterized. Therefore, herein, we describe two methods to determine temporal and spatial expression of Calpain 2 during Xenopus development, namely, RT-PCR and whole-mount in situ hybridization (WISH). In addition, indirect immunofluorescence (IF) is described to determine translocation to the plasma membrane, which correlates with activity levels of Calpain 2.