Browsing by Author "Koutsoupakis, Constantinos"
Now showing items 1-5 of 5
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Aldoxime dehydratase: Probing the heme environment involved in the synthesis of the carbon-nitrogen triple bond
Pinakoulaki, Eftychia; Koutsoupakis, Constantinos; Sawai, H.; Pavlou, A.; Kato, Y.; Asano, Y.; Aono, S. (2011)Fourier transform infrared (FTIR) spectra, "light" minus "dark" difference FTIR spectra, and time-resolved step-scan (TRS 2) FTIR spectra are reported for carbonmonoxy aldoxime dehydratase. Two C-O modes of heme at 1945 ...
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Article
Decay of the transient CuB-CO complex is accompanied by formation of the heme Fe-CO complex of cytochrome cbb3-CO at ambient temperature: Evidence from time-resolved fourier transform infrared spectroscopy
Stavrakis, Stavros; Koutsoupakis, Constantinos; Pinakoulaki, Eftychia; Urban, A.; Saraste, M.; Varotsis, Constantinos (2002)Time-resolved step-scan Fourier infrared spectroscopy has been used to study the CO-bound cbb3-type cytochrome c oxidase from Pseudomonas stutzeri at room temperature. We observe a single band in the FTIR spectrum at 1956 ...
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Article
Observation of the equilibrium CuB-CO complex and functional implications of the transient heme a3 propionates in cytochrome ba3-CO from Thermus thermophilus. Fourier transform infrared (FTIR) and time-resolved step-scan FTIR studies
Koutsoupakis, Constantinos; Stavrakis, Stavros; Pinakoulaki, Eftychia; Soulimane, T.; Varotsis, Constantinos (2002)We report the first evidence for the existence of the equilibrium CuB 1+-CO species of CO-bound reduced cytochrome ba3 from Thermus thermophilus at room temperature. The frequency of the C-O stretching mode of CuB 1+-CO ...
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Structural dynamics of heme-copper oxidases and nitric oxide reductases: Time-resolved step-scan Fourier transform infrared and time-resolved resonance Raman studies
Pinakoulaki, Eftychia; Koutsoupakis, Constantinos; Stavrakis, Stavros; Aggelaki, Marialena; Papadopoulos, George Angelos; Daskalakis, Vangelis; Varotsis, Constantinos (2005)Of the spectroscopic methods available for the characterization of the dynamics of heme protein active sites, time-resolved resonance Raman spectroscopy (TR3) is a powerful technique because excitation within the heme π-π* ...
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Time-resolved step-scan Fourier transform infrared investigation of heme-copper oxidases: Implications for O2 input and H 2O/H+ output channels
Koutsoupakis, Constantinos; Pinakoulaki, Eftychia; Stavrakis, Stavros; Daskalakis, Vangelis; Varotsis, Constantinos (2004)We have applied FTIR and time-resolved step-scan Fourier transform infrared (TRS2-FTIR) spectroscopy to investigate the dynamics of the heme-CuB binuclear center and the protein dynamics of mammalian aa3, Pseudomonas ...