Browsing by Subject "Drosophila melanogaster"

Article

The bacterial metabolite 2-aminoacetophenone promotes association of pathogenic bacteria with flies

Kapsetaki, Stefania Elisavet; Tzelepis, Ilias; Avgousti, Kalodoti; Livadaras, Ioannis; Garantonakis, Nikos G.; Varikou, Kyriaki N.; Apidianakis, Yiorgos (2014)

Bacteria contaminate insects and secrete metabolites that may affect insect behaviour and potentially fitness through unknown mechanisms. Here we show that the 'grape-like' odorant 2-aminoacetophenone (2AA), secreted by ...

Article

Challenge of Drosophila melanogaster with Cryptococcus neoformans and role of the innate immune response

Apidianakis, Yiorgos; Rahme, L. G.; Heitman, J.; Ausubel, F. M.; Calderwood, S. B.; Mylonakis, E. (2004)

We found that the ingestion of Cryptococcus neoformans by Drosophila melanogaster resulted in the death of the fly but that the ingestion of Saccharomyces cerevisiae or the nonpathogenic Cryptococcus kuetzingii or Cryptococcus ...

Article

Drosophila and the hallmarks of cancer

Christofi, T.; Apidianakis, Yiorgos (2013)

: Cancer was the disease of the twentieth century. Today it is still a leading cause of death worldwide despite being intensively investigated. Abundant knowledge exists regarding the pathological and molecular mechanisms ...

Article

Drosophila immune priming against Pseudomonas aeruginosa is short-lasting and depends on cellular and humoral immunity

Apidianakis, Yiorgos; Christofi, T. (2013)

Immune responses are traditionally divided into the innate and the adaptive arm, both of which are present in vertebrates, while only the innate arm is found in invertebrates. Immune priming experiments in Drosophila ...

Article

Drosophila melanogaster as a model for human intestinal infection and pathology

Apidianakis, Yiorgos; Rahme, L. G. (2011)

Recent findings concerning Drosophila melanogaster intestinal pathology suggest that this model is well suited for the study of intestinal stem cell physiology during aging, stress and infection. Despite the physiological ...

Article

Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa infection

Apidianakis, Yiorgos; Rahme, L. G. (2009)

Conservation of host signaling pathways and tissue physiology between Drosophila melanogaster and mammals allows for the modeling of human host-pathogen interactions in Drosophila. Here we present the use of genetically ...

Article

Drosophila melanogaster: A first step and a stepping-stone to anti-infectives

Tzelepis, Ilias; Kapsetaki, Stefania Elisavet; Panayidou, S.; Apidianakis, Yiorgos (2013)

Following an expansion in the antibiotic drug discovery in the previous century, we now face a bottleneck in the production of new anti-infective drugs. Traditionally, chemical libraries are screened either using in vitro ...

Article

Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes

Markstein, M.; Pitsouli, Chrysoula; Villalta, C.; Celniker, S. E.; Perrimon, N. (2008)

A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase ...

Article

Human pathogenic bacteria, fungi, and viruses in Drosophila

Panayidou, S.; Ioannidou, E.; Apidianakis, Yiorgos (2014)

Drosophila has been the invertebrate model organism of choice for the study of innate immune responses during the past few decades. Many Drosophila-microbe interaction studies have helped to define innate immunity pathways, ...

Article

In vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injury

Constantinou, Constantina; Apidianakis, Yiorgos; Psychogios, N.; Righi, V.; Mindrinos, M. N.; Khan, N.; Swartz, H. M.; Szeto, H. H.; Tompkins, R. G.; Rahme, L. G.; Aria Tzika, A. (2016)

Trauma is the most common cause of mortality among individuals aged between 1 and 44 years and the third leading cause of mortality overall in the US. In this study, we examined the effects of trauma on the expression of ...

Article

In vivo high-resolution magic angle spinning magnetic resonance spectroscopy of Drosophila melanogaster at 14.1 T shows trauma in aging and in innate immune-deficiency is linked to reduced insulin signaling

Righi, V.; Apidianakis, Yiorgos; Mintzopoulos, D.; Astrakas, L.; Rahme, L. G.; Tzika, A. A. (2010)

In vivo magnetic resonance spectroscopy (MRS), a non-destructive biochemical tool for investigating live organisms, has yet to be used in the fruit fly Drosophila melanogaster, a useful model organism for investigating ...

Article

In vivo high-resolution magic angle spinning proton NMR spectroscopy of Drosophila melanogaster flies as a model system to investigate mitochondrial dysfunction in Drosophila GST2 mutants

Righi, V.; Apidianakis, Yiorgos; Psychogios, N.; Rahme, L. G.; Tompkins, R. G.; Tzika, A. A. (2014)

In vivo nuclear magnetic resonance spectroscopy (NMR), a non-destructive biochemical tool used for investigating live organisms, has recently been performed in studies of the fruit fly Drosophila melanogaster, a useful ...

Article

In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography

Vinegoni, C.; Pitsouli, Chrysoula; Razansky, D.; Perrimon, N.; Ntziachristos, V. (2008)

We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in ...

Article

Involvement of skeletal muscle gene regulatory network in susceptibility to wound infection following trauma

Apidianakis, Yiorgos; Mindrinos, M. N.; Xiao, W.; Tegos, G. P.; Papisov, M. I.; Hamblin, M. R.; Davis, R. W.; Tompkins, R. G.; Rahme, L. G. (2007)

Despite recent advances in our understanding the pathophysiology of trauma, the basis of the predisposition of trauma patients to infection remains unclear. A Drosophila melanogaster/Pseudomonas aeruginosa injury and ...

Article

Magnetic resonance spectroscopy of live drosophila melanogaster using magic angle spinning

Righi, V.; Apidianakis, Yiorgos; Rahme, L. G.; Tzika, A. A. (2010)

High-Resolution Magic Angle Spinning (HRMAS) proton magnetic resonance spectroscopy (1H-MRS) is a novel non-destructive technique that improves spectral line-widths and allows high-resolution spectra to be obtained from ...

Article

Magnetic resonance spectroscopy of live Drosophila melanogaster using magic angle spinning.

Righi, V.; Apidianakis, Yiorgos; Rahme, L. G.; Tzika, A. A. (2010)

High-Resolution Magic Angle Spinning (HRMAS) proton magnetic resonance spectroscopy ((1)H-MRS) is a novel non-destructive technique that improves spectral line-widths and allows high-resolution spectra to be obtained from ...

Article

Mesoscopic fluorescence tomography for in-vivo imaging of developing Drosophila.

Vinegoni, C.; Razansky, D.; Pitsouli, Chrysoula; Perrimon, N.; Ntziachristos, V.; Weissleder, R. (2009)

Visualizing developing organ formation as well as progession and treatment of disease often heavily relies on the ability to optically interrogate molecular and functional changes in intact living organisms. Most existing ...

Article

Neuralized Encodes a Peripheral Membrane Protein Involved in Delta Signaling and Endocytosis

Pavlopoulos, Elias; Pitsouli, Chrysoula; Klueg, K. M.; Muskavitch, M. A. T.; Moschonas, Nicholas K.; Delidakis, Christos (2001)

Activation of the Notch (N) receptor involves an intracellular proteolytic step triggered by shedding of the extracellular N domain (N-EC) upon ligand interaction. The ligand Dl has been proposed to effect this N-EC shedding ...

Article

The pathogenic properties of a novel and conserved gene product, KerV, in proteobacteria

An, D.; Apidianakis, Yiorgos; Boechat, A. L.; Baldini, R. L.; Goumnerov, B. C.; Rahme, L. G. (2009)

Identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. In this study, we uncover such a factor, termed KerV, in Proteobacteria. Experiments ...

Article

Profiling early infection responses: Pseudomonas aeruginosa eludes host defenses by suppressing antimocrobial peptide gene expression

Apidianakis, Yiorgos; Mindrinos, M. N.; Xiao, W.; Lau, G. W.; Baldini, R. L.; Davis, R. W.; Rahme, L. G. (2005)

Insights into the host factors and mechanisms mediating the primary host responses after pathogen presentation remain limited, due in part to the complexity and genetic intractability of host systems. Here, we employ the ...