Browsing by Subject "down regulation"

Article

Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells

Antoniades, Ioanna; Stylianou, Panayiota; Christodoulou, Neophytos; Skourides, Paris A. (2017)

We previously identified focal adhesion kinase (FAK) as an important regulator of ciliogenesis in multiciliated cells. FAK and other focal adhesion (FA) proteins associate with the basal bodies and their striated rootlets ...

Article

Calpain2 protease: A new member of the Wnt/Ca2+ pathway modulating convergent extension movements in Xenopus

Zanardelli, Sara; Christodoulou, Neophytos; Skourides, Paris A. (2013)

Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate several physiological processes by limited cleavage of different substrates. The role of Calpain2 in embryogenesis is not clear with ...

Article

CXCR4 mediates entry and productive infection of syncytia-inducing (x4) hiv-1 strains in primary macrophages

Valentin, A.; Trivedi, H.; Lu, W.; Kostrikis, Leontios G.; Pavlakis, G. N. (2000)

CCR5 and CXCR4 are the main coreceptors for non-syncytia-inducing (NSI) and syncytia-inducing (SI) HIV-1 strains, respectively. NSI HIV-1 isolates do not infect either human lymphoid or monocytoid cell lines, and this ...

Article

D-alpha-tocopheryl polyethylene glycol succinate (TPGS) induces cell cycle arrest and apoptosis selectively in Survivin-overexpressing breast cancer cells

Neophytou, Christiana M.; Constantinou, Constantina; Papageorgis, P.; Constantinou, Andreas I. (2014)

d-alpha-tocopheryl polyethylene glycol succinate (TPGS) is a vitamin E derivative that has been intensively applied as a vehicle for drug delivery systems to enhance drug solubility and increase the oral bioavailability ...

Article

Down-regulation of glutatione S-transferase α 4 (hGSTA4) in the muscle of thermally injured patients is indicative of susceptibility to bacterial infection

Apidianakis, Yiorgos; Que, Y. -A; Xu, W.; Tegos, G. P.; Zimniak, P.; Hamblin, M. R.; Tompkins, R. G.; Xiao, W.; Rahme, L. G. (2012)

Patients with severe burns are highly susceptible to bacterial infection. While immunosuppression facilitates infection, the contribution of soft tissues to infection beyond providing a portal for bacterial entry remains ...

Article

The Ink4/Arf locus is a barrier for iPS cell reprogramming

Li, H.; Collado, M.; Villasante, A.; Strati, K.; Ortega, S.; Cãamero, M.; Blasco, M. A.; Serrano, M. (2009)

The mechanisms involved in the reprogramming of differentiated cells into induced pluripotent stem (iPS) cells by the three transcription factors Oct4 (also known as Pou5f1), Klf4 and Sox2 remain poorly understood. The ...

Article

Loss of Nat4 and its associated histone H4 N-terminal acetylation mediates calorie restriction-induced longevity

Molina-Serrano, D.; Schiza, V.; Demosthenous, Christis; Stavrou, Emmanouil; Oppelt, J.; Kyriakou, DImitris; Liu, W.; Zisser, G.; Bergler, H.; Dang, W.; Kirmizis, Antonis (2016)

Changes in histone modifications are an attractive model through which environmental signals, such as diet, could be integrated in the cell for regulating its lifespan. However, evidence linking dietary interventions with ...

Article

Making the Connection: Ciliary Adhesion Complexes Anchor Basal Bodies to the Actin Cytoskeleton

Antoniades, Ioanna; Stylianou, Panayiota; Skourides, Paris A. (2014)

Cilia have been associated with diverse developmental and physiological processes, and defects in cilia underlie a number of genetic conditions. Several lines of evidence support a critical role of the actin cytoskeleton ...

Article

A miR-1207-5p binding site polymorphism abolishes regulation of HBEGF and is associated with disease severity in CFHR5 nephropathy

Papagregoriou, Gregory N.; Erguler, K.; Dweep, H.; Voskarides, Konstantinos; Koupepidou, P.; Athanasiou, Yiannis; Pierides, Alkis M.; Gretz, N.; Felekkis, Kyriacos N.; Constantinou-Deltas, Constantinos D. (2012)

Heparin binding epidermal growth factor (HBEGF) is expressed in podocytes and was shown to play a role in glomerular physiology. MicroRNA binding sites on the 3′UTR of HBEGF were predicted using miRWalk algorithm and ...

Article

The mouse Foxi3 transcription factor is necessary for the development of posterior placodes

Birol, O.; Ohyama, T.; Edlund, R. K.; Drakou, Katerina; Georgiades, Pantelis; Groves, A. K. (2016)

The inner ear develops from the otic placode, one of the cranial placodes that arise from a region of ectoderm adjacent to the anterior neural plate called the pre-placodal domain. We have identified a Forkhead family ...

Article

New miRNA profiles accurately distinguish renal cell carcinomas and upper tract urothelial carcinomas from the normal kidney

Zaravinos, Apostolos; Lambrou, George I.; Mourmouras, Nikos; Katafygiotis, Patroklos; Papagregoriou, Gregory N.; Giannikou, Krinio; Delakas, Dimitrios S.; Constantinou-Deltas, Constantinos D. (2014)

Background: Upper tract urothelial carcinomas (UT-UC) can invade the pelvicalyceal system making differential diagnosis of the various histologically distinct renal cell carcinoma (RCC) subtypes and UT-UC, difficult. Correct ...

Article

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis

Kypri, Elena; Christodoulou, A.; Maimaris, G.; Lethan, M.; Markaki, M.; Lysandrou, C.; Lederer, C. W.; Tavernarakis, N.; Geimer, S.; Pedersen, L. B.; Santama, Niovi (2014)

Nucleotide-binding proteins Nubp1 and Nubp2 are MRP/MinD-type P-loop NTPases with sequence similarity to bacterial division site-determining proteins and are conserved, essential proteins throughout the Eukaryotes. They ...

Article

Overexpression of the m4 and mα genes of the E(spl)-Complex antagonizes Notch mediated lateral inhibition

Apidianakis, Yiorgos; Nagel, A. C.; Chalkiadaki, Angeliki; Preiss, A.; Delidakis, Christos (1999)

Intercellular signalling mediated by Notch proteins is crucial to many cell fate decisions in metazoans. Its profound effects on cell fate and proliferation require that a complex set of responses involving positive and ...

Article

Pirfenidone normalizes the tumor microenvironment to improve chemotherapy

Polydorou, C.; Mpekris, F.; Papageorgis, P.; Voutouri, C.; Stylianopoulos, T. (2017)

Normalization of the tumor microenvironment by selectively targeting components of the tumor extracellular matrix has been recently proposed to have the potential to decompress tumor blood vessels, increase vessel perfusion ...

Article

A quorum sensing regulated small volatile molecule reduces acute virulence and promotes chronic infection phenotypes

Kesarwani, M.; Hazan, R.; He, J.; Que, Y.; Apidianakis, Yiorgos; Lesic, B.; Xiao, G.; Dekimpe, V.; Milot, S.; Déziel, Eric; Lépine, F.; Rahme, L. G. (2011)

A significant number of environmental microorganisms can cause serious, even fatal, acute and chronic infections in humans. The severity and outcome of each type of infection depends on the expression of specific bacterial ...

Article

Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and urokinase-plasminogen activator (uPA)

Gkretsi, V.; Stylianou, A.; Stylianopoulos, T. (2017)

A hallmark of cancer cells is their ability to invade surrounding tissues and form metastases. Cell-extracellular matrix (ECM)-adhesion proteins are crucial in metastasis, connecting tumor ECM with actin cytoskeleton thus ...

Article

Xenopus laevis nucleotide binding protein 1 (xNubp1) is important for convergent extension movements and controls ciliogenesis via regulation of the actin cytoskeleton

Ioannou, Androulla; Santama, Niovi; Skourides, Paris A. (2013)

Nucleotide binding protein 1 (Nubp1) is a highly conserved phosphate loop (P-loop) ATPase involved in diverse processes including iron-sulfur protein assembly, centrosome duplication and lung development. Here, we report ...