Browsing by Subject "in vivo study"

Article

Activation of endogenous FAK via expression of its amino terminal domain in xenopus embryos

Petridou, Nicoletta I.; Stylianou, Panayiota; Christodoulou, Neophytos; Rhoads, Daniel S.; Guan, Junlin; Skourides, Paris A. (2012)

Background: The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic ...

Article

Coevolution of solid stress and interstitial fluid pressure in tumors during progression: Implications for vascular collapse

Stylianopoulos, T.; Martin, J. D.; Snuderl, M.; Mpekris, F.; Jain, S. R.; Jain, R. K. (2013)

The stress harbored by the solid phase of tumors is known as solid stress. Solid stress can be either applied externally by the surrounding normal tissue or induced by the tumor itself due to its growth. Fluid pressure is ...

Article

A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis

Petridou, Nicoletta I.; Stylianou, Panayiota; Skourides, Paris A. (2013)

FAK is a non-receptor tyrosine kinase involved in a wide variety of biological processes and crucial for embryonic development. In this manuscript, we report the generation of a new FAK dominant negative (FF), composed of ...

Article

Ets2-dependent trophoblast signalling is required for gastrulation progression after primitive streak initiation

Polydorou, Christiana; Georgiades, Pantelis (2013)

Although extraembryonic ectoderm trophoblast signals the embryo for primitive streak initiation, a prerequisite for gastrulation, it is unknown whether it also signals for the progression of gastrulation after primitive ...

Article

FOXO3a is broadly neuroprotective in vitro and in vivo against insults implicated in motor neuron diseases

Mojsilovic-Petrovic, J.; Nedelsky, N.; Boccitto, M.; Mano, I.; Georgiades, Savvas N.; Zhou, W.; Liu, Y.; Neve, R. L.; Taylor, J. P.; Driscoll, M.; Clardy, J.; Merry, D.; Kalb, R. G. (2009)

Aging is a risk factor for the development of adult-onset neurodegenerative diseases. Although some of the molecular pathways regulating longevity and stress resistance in lower organisms are defined (i.e., those activating ...

Article

Histone H3 tail clipping regulates gene expression

Santos-Rosa, H.; Kirmizis, Antonis; Nelson, C.; Bartke, T.; Saksouk, N.; Cote, J.; Kouzarides, T. (2009)

Induction of gene expression in yeast and human cells involves changes in the histone modifications associated with promoters. Here we identify a histone H3 endopeptidase activity in Saccharomyces cerevisiae that may ...

Article

The HPV16 oncogenes cause aberrant stem cell mobilization

Michael, S.; Lambert, P. F.; Strati, K. (2013)

Human Papilloma Virus related epithelial cancers have been speculated to derive from virus-infected tissue stem cells. Stem cells also are thought to provide a reservoir of latently infected cells that can persist for long ...

Article

Hyaluronan-Derived Swelling of Solid Tumors, the Contribution of Collagen and Cancer Cells, and Implications for Cancer Therapy

Voutouri, C.; Polydorou, C.; Papageorgis, P.; Gkretsi, V.; Stylianopoulos, T. (2016)

Despite the important role that mechanical forces play in tumor growth and therapy, the contribution of swelling to tumor mechanopathology remains unexplored. Tumors rich in hyaluronan exhibit a highly negative fixed charge ...

Article

Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals

Stylianou, Panayiota; Skourides, Paris A. (2009)

Mesoderm migration is a well studied morphogenetic movement that takes place during Xenopus gastrulation. The study of mesoderm migration and other morphogenetic movements has been primarily based on in vitro assays due ...

Article

In vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injury

Constantinou, Constantina; Apidianakis, Yiorgos; Psychogios, N.; Righi, V.; Mindrinos, M. N.; Khan, N.; Swartz, H. M.; Szeto, H. H.; Tompkins, R. G.; Rahme, L. G.; Aria Tzika, A. (2016)

Trauma is the most common cause of mortality among individuals aged between 1 and 44 years and the third leading cause of mortality overall in the US. In this study, we examined the effects of trauma on the expression of ...

Article

In vivo high-resolution magic angle spinning magnetic resonance spectroscopy of Drosophila melanogaster at 14.1 T shows trauma in aging and in innate immune-deficiency is linked to reduced insulin signaling

Righi, V.; Apidianakis, Yiorgos; Mintzopoulos, D.; Astrakas, L.; Rahme, L. G.; Tzika, A. A. (2010)

In vivo magnetic resonance spectroscopy (MRS), a non-destructive biochemical tool for investigating live organisms, has yet to be used in the fruit fly Drosophila melanogaster, a useful model organism for investigating ...

Article

In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography

Vinegoni, C.; Pitsouli, Chrysoula; Razansky, D.; Perrimon, N.; Ntziachristos, V. (2008)

We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in ...

Article

Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo

Charalambous, Anna; Andreou, Maria I.; Skourides, Paris A. (2009)

We describe an intein based method to site-specifically conjugate Quantum Dots (QDs) to target proteins in vivo. This approach allows the covalent conjugation of any nanostructure and/or nanodevice to any protein and thus ...

Article

Model-based tumor growth dynamics and therapy response in a mouse model of de novo carcinogenesis

Loizides, Constantinos A.; Iacovides, D.; Hadjiandreou, M. M.; Rizki, G.; Achilleos, Achilleas P.; Strati, K.; Mitsis, Georgios D. (2015)

Tumorigenesis is a complex, multistep process that depends on numerous alterations within the cell and contribution from the surrounding stroma. The ability to model macroscopic tumor evolution with high fidelity may ...

Article

Multistage nanoparticle delivery system for deep penetration into tumor tissue

Wong, C.; Stylianopoulos, T.; Cui, J.; Martin, J.; Chauhan, V. P.; Jiang, W.; Popovic, Z.; Jain, R. K.; Bawendi, M. G.; Fukumura, D. (2011)

Current Food and Drug Administration-approved cancer nanotherapeutics, which passively accumulate around leaky regions of the tumor vasculature because of an enhanced permeation and retention (EPR) effect, have provided ...

Article

A quorum sensing regulated small volatile molecule reduces acute virulence and promotes chronic infection phenotypes

Kesarwani, M.; Hazan, R.; He, J.; Que, Y.; Apidianakis, Yiorgos; Lesic, B.; Xiao, G.; Dekimpe, V.; Milot, S.; Déziel, Eric; Lépine, F.; Rahme, L. G. (2011)

A significant number of environmental microorganisms can cause serious, even fatal, acute and chronic infections in humans. The severity and outcome of each type of infection depends on the expression of specific bacterial ...

Article

Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo

Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2011)

Background: Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within ...

Article

Targeting IL13Ralpha2 activates STAT6-TP63 pathway to suppress breast cancer lung metastasis

Papageorgis, P.; Ozturk, S.; Lambert, A. W.; Neophytou, Christiana M.; Tzatsos, Alexandros; Wong, C. K.; Thiagalingam, S.; Constantinou, Andreas I. (2015)

Introduction: Basal-like breast cancer (BLBC) is an aggressive subtype often characterized by distant metastasis, poor patient prognosis, and limited treatment options. Therefore, the discovery of alternative targets to ...