dc.contributor.author | Nicolaou, I. N. | en |
dc.contributor.author | Kapnissi‐Christodoulou, Constantina P. | en |
dc.creator | Nicolaou, I. N. | en |
dc.creator | Kapnissi‐Christodoulou, Constantina P. | en |
dc.date.accessioned | 2019-11-21T06:21:46Z | |
dc.date.available | 2019-11-21T06:21:46Z | |
dc.date.issued | 2012 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/55913 | |
dc.description.abstract | A capillary zone electrophoresis-electrospray ionization-mass spectrometry (CZE-ESI-MS) method was developed for the analysis of the acetylcholinesterase inhibitor rivastigmine. Several electrophoretic and ESI-MS parameters were investigated in order to improve sensitivity. These parameters were categorized in three areas: (i) background electrolyte (BGE) parameters, (ii) sheath liquid parameters, and (iii) spray chamber parameters. The optimized results were obtained by using 40-mM ammonium acetate at pH 9 as BGE, a sheath liquid of 1% acetic acid in water:MeOH (50:50 v/v) at a flow rate of 10 µL/min, and a drying gas flow rate that was set at 6 L/min and at a temperature of 200°C. These parameters provided limit of detection and limit of quantitation of 2.8 ng/mL and 8.4 ng/mL, respectively. The optimal CZE-ESI-MS conditions were applied to a plasma sample obtained from an Alzheimer's disease patient following rivastigmine patch administration, and the mean (±standard deviation) plasma concentration was estimated to be 14.6 (±1.7) ng/mL. Several sample preparation procedures were examined, and solid-phase extraction using a C18 cartridge proved to be the most effective procedure, since higher sensitivity and recovery were obtained. In addition, precision was evaluated based on migration time and peak area in plasma, and the relative standard deviations were in the range of 0.10-0.16% and 0.62-9.0%, respectively. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | en |
dc.source | Electrophoresis | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84858994977&doi=10.1002%2felps.201100286&partnerID=40&md5=4111864c34ad0fe33928c2d2a12a938c | |
dc.subject | article | en |
dc.subject | Humans | en |
dc.subject | controlled study | en |
dc.subject | quantitative analysis | en |
dc.subject | sensitivity analysis | en |
dc.subject | statistical analysis | en |
dc.subject | Reproducibility of Results | en |
dc.subject | drug blood level | en |
dc.subject | blood sampling | en |
dc.subject | drug determination | en |
dc.subject | accuracy | en |
dc.subject | Alzheimer disease | en |
dc.subject | Calibration | en |
dc.subject | Gases | en |
dc.subject | temperature | en |
dc.subject | Mass spectrometry | en |
dc.subject | pH | en |
dc.subject | flow rate | en |
dc.subject | water | en |
dc.subject | Hydrogen-Ion Concentration | en |
dc.subject | process optimization | en |
dc.subject | Osmolar Concentration | en |
dc.subject | Limit of Detection | en |
dc.subject | electrospray mass spectrometry | en |
dc.subject | Spectrometry, Mass, Electrospray Ionization | en |
dc.subject | electrolyte | en |
dc.subject | acetic acid | en |
dc.subject | solid phase extraction | en |
dc.subject | Electrophoresis, Capillary | en |
dc.subject | Capillary electrophoresis | en |
dc.subject | capillary zone electrophoresis | en |
dc.subject | analytical parameters | en |
dc.subject | migration time | en |
dc.subject | Acetylcholinesterase inhibitors | en |
dc.subject | ammonium acetate | en |
dc.subject | drying gas flow rate | en |
dc.subject | gas flow | en |
dc.subject | neostigmine | en |
dc.subject | peak area in plasma | en |
dc.subject | Phenylcarbamates | en |
dc.subject | Plasma sample preparation | en |
dc.subject | Rivastigmine | en |
dc.subject | shealth liquid parameter | en |
dc.subject | spray chamber parameter | en |
dc.title | Development of a capillary electrophoresis-mass spectrometry method for the determination of rivastigmine in human plasma - Optimization of the limits of detection and quantitation | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1002/elps.201100286 | |
dc.description.volume | 33 | |
dc.description.issue | 4 | |
dc.description.startingpage | 644 | |
dc.description.endingpage | 652 | |
dc.author.faculty | 002 Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Χημείας / Department of Chemistry | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Manufacturers: Sigma Aldrich, Germany | en |
dc.description.notes | Cited By :4</p> | en |
dc.source.abbreviation | Electrophoresis | en |
dc.contributor.orcid | Kapnissi‐Christodoulou, Constantina P. [0000-0003-3755-1052] | |
dc.gnosis.orcid | 0000-0003-3755-1052 | |