dc.contributor.author | Russel, Mohammad | en |
dc.contributor.author | Sophocleous, Marios | en |
dc.contributor.author | JiaJia, Shan | en |
dc.contributor.author | Xu, Weiping | en |
dc.contributor.author | Xiao, Lehui | en |
dc.contributor.author | Maskow, Thomas | en |
dc.contributor.author | Alam, Mahbub | en |
dc.contributor.author | Georgiou, Julius | en |
dc.creator | Russel, Mohammad | en |
dc.creator | Sophocleous, Marios | en |
dc.creator | JiaJia, Shan | en |
dc.creator | Xu, Weiping | en |
dc.creator | Xiao, Lehui | en |
dc.creator | Maskow, Thomas | en |
dc.creator | Alam, Mahbub | en |
dc.creator | Georgiou, Julius | en |
dc.date.accessioned | 2021-01-26T09:45:20Z | |
dc.date.available | 2021-01-26T09:45:20Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0003-2670 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/63175 | |
dc.description.abstract | This paper describes a novel technique to quantify and identify bacterial cultures of Bacillus Subtilis (2.10–1.30 × 109 CFU mL−1) and Escherichia Coli (1.60–1.00 × 109 CFU mL−1), in corn oil using dielectric spectroscopy at elevated frequencies of 0.0100–20.0 GHz. This technique is using the electrophysiological/biophysical differences (e.g. gram positive and gram negative) between various bacteria types, as a basis to distinguish between bacteria concentrations and bacteria types. A close-ended, coaxial probe of 20.0 mm long sample-holder was developed and used to calculate the dielectric constant from the measured S parameters of the bacterial cultures, using the Nicolson-Ross-Weir method. This technique shows a linear relationship (r2 ≥ 0.999) between the dielectric constant and the cell concentration, at 16.0 GHz. The sensitivity of the technique is 0.177 × 109 (CFU mL−1)−1 for B. Subtilis (with a size of 10.0 × 1.00 μm), 0.322 × 109 (CFU mL−1)−1 for E. Coli (with a size of 2.00 × 0.500 μm) and 0.913 × 109 (CFU mL−1) −1 for their 1:1 mixture, while the response time is 60.0s. The dependency of dielectric constant on the bacterial cell concentration at a given frequency can be potentially exploited for measuring bacterial concentrations and biophysical differences. | en |
dc.language.iso | en | en |
dc.source | Analytica Chimica Acta | en |
dc.source.uri | http://www.sciencedirect.com/science/article/pii/S0003267018305312 | |
dc.title | High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1016/j.aca.2018.04.045 | |
dc.description.volume | 1028 | |
dc.description.startingpage | 86 | |
dc.description.endingpage | 95 | |
dc.author.faculty | Πολυτεχνική Σχολή / Faculty of Engineering | |
dc.author.department | Τμήμα Ηλεκτρολόγων Μηχανικών και Μηχανικών Υπολογιστών / Department of Electrical and Computer Engineering | |
dc.type.uhtype | Article | en |
dc.source.abbreviation | Analytica Chimica Acta | en |
dc.contributor.orcid | Georgiou, Julius [0000-0002-7474-5449] | |
dc.contributor.orcid | Sophocleous, Marios [0000-0002-9669-0581] | |
dc.gnosis.orcid | 0000-0002-7474-5449 | |
dc.gnosis.orcid | 0000-0002-9669-0581 | |