Browsing by Author "Skourides, Paris A."
Now showing items 1-20 of 27
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Article
1,2,3-Dithiazoles – new reversible melanin synthesis inhibitors: a chemical genomics study
Charalambous, Anna; Koyioni, Maria G.; Antoniades, Ioanna A.; Pegeioti, Despoina; Eleftheriou, Iro; Michaelidou, Sophia S.; Amelichev, Stanislav A.; Konstantinova, Lidia S.; Rakitin, Oleg A.; Koutentis, Panayiotis Andreas; Skourides, Paris A. (2015)
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Article
1,2,3-Dithiazoles-new reversible melanin synthesis inhibitors: A chemical genomics study
Charalambous, Anna; Koyioni, Maria; Antoniades, Ioanna; Pegeioti, Despoina; Eleftheriou, Iro; Michaelidou, Sophia S.; Amelichev, Stanislav A.; Konstantinova, L. S.; Rakitin, O. A.; Koutentis, Panayiotis Andreas; Skourides, Paris A. (2015)A chemical genomic screen of an in-house library of small molecule heterocycles was carried out using Xenopus laevis embryos. This led to the identification of N-(4-chloro-5H-1,2,3-dithiazol-5-ylidene)-4-methoxyaniline ...
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Article
40LoVe and Samba are involved in Xenopusneural development and functionally distinct from hnRNP AB
Andreou, Maria I.; Yan, C. Y. I.; Skourides, Paris A. (2014)Heterogeneous nuclear ribonucleoproteins (hnRNPs) comprise a large group of modular RNA-binding proteins classified according to their conserved domains. This modular nature, coupled with a large choice of alternative ...
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Article
A ligand-independent integrin β1 mechanosensory complex guides spindle orientation
Petridou, Nicoletta I.; Skourides, Paris A. (2016)Control of spindle orientation is a fundamental process for embryonic development, morphogenesis and tissue homeostasis, while defects are associated with tumorigenesis and other diseases. Force sensing is one of the ...
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Article
Activation of endogenous FAK via expression of its amino terminal domain in xenopus embryos
Petridou, Nicoletta I.; Stylianou, Panayiota; Christodoulou, Neophytos; Rhoads, Daniel S.; Guan, Junlin; Skourides, Paris A. (2012)Background: The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic ...
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Article
Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells
Antoniades, Ioanna; Stylianou, Panayiota; Christodoulou, Neophytos; Skourides, Paris A. (2017)We previously identified focal adhesion kinase (FAK) as an important regulator of ciliogenesis in multiciliated cells. FAK and other focal adhesion (FA) proteins associate with the basal bodies and their striated rootlets ...
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Article
Calpain2 protease: A new member of the Wnt/Ca2+ pathway modulating convergent extension movements in Xenopus
Zanardelli, Sara; Christodoulou, Neophytos; Skourides, Paris A. (2013)Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate several physiological processes by limited cleavage of different substrates. The role of Calpain2 in embryogenesis is not clear with ...
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Article
Cell-Autonomous Ca(2+) Flashes Elicit Pulsed Contractions of an Apical Actin Network to Drive Apical Constriction during Neural Tube Closure
Christodoulou, Neophytos; Skourides, Paris A. (2015)Neurulation is a critical period in all vertebrates and results in the formation of the neural tube, which gives rise to the CNS. Apical constriction is one of the fundamental morphogenetic movements that drives neural ...
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Article
Comparing intracellular stability and targeting of sulfobetaine quantum dots with other surface chemistries in live cells
Muro, E.; Fragola, A.; Pons, T.; Lequeux, N.; Ioannou, Androulla; Skourides, Paris A.; Dubertret, B. (2012)The in vivo labeling of intracellular components with quantum dots (QDs) is very limited because of QD aggregation in the cell cytoplasm and/or QD confinement into lysosomal compartments. In order to improve intracellular ...
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Book Chapter
Determining Temporal and Spatial Expression of Calpains in Amphibians
Charalambous, Anna; Antoniades, Ioanna; Christodoulou, Neophytos; Zanardelli, Sara; Skourides, Paris A. (Springer, 2019)Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that Calpains have been identified in the Xenopus genome, ...
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Article
A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis
Petridou, Nicoletta I.; Stylianou, Panayiota; Skourides, Paris A. (2013)FAK is a non-receptor tyrosine kinase involved in a wide variety of biological processes and crucial for embryonic development. In this manuscript, we report the generation of a new FAK dominant negative (FF), composed of ...
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Article
FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis
Petridou, Nicoletta I.; Skourides, Paris A. (2014)Spindle orientation is critical for proper morphogenesis of organs and tissues as well as for the maintenance of tissue morphology. Although significant progress has been made in understanding the mechanisms linking the ...
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Article
High-resolution whole-mount in situ hybridization using quantum dot nanocrystals
Ioannou, Androulla; Eleftheriou, Iro; Lubatti, Andrea; Charalambous, Anna; Skourides, Paris A. (2012)The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically ...
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Article
Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals
Stylianou, Panayiota; Skourides, Paris A. (2009)Mesoderm migration is a well studied morphogenetic movement that takes place during Xenopus gastrulation. The study of mesoderm migration and other morphogenetic movements has been primarily based on in vitro assays due ...
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Article
In vivo imaging of quantum dots encapsulated in phospholipid micelles
Dubertret, B.; Skourides, Paris A.; Norris, D. J.; Brivanlou, A. H.; Libchaber, A.; Noireaux, V. (2002)Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this ...
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Article
In vivo, site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing
Charalambous, Anna; Andreou, Maria I.; Antoniades, Ioanna; Christodoulou, Neophytos; Skourides, Paris A. (2012)The ability to target proteins with nanostructures and/or nanodevices in vivo is important for understanding and controlling their biological function. Quantum dots (QDs) serve as an ideal model nanostructure due to their ...
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Article
Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo
Charalambous, Anna; Andreou, Maria I.; Skourides, Paris A. (2009)We describe an intein based method to site-specifically conjugate Quantum Dots (QDs) to target proteins in vivo. This approach allows the covalent conjugation of any nanostructure and/or nanodevice to any protein and thus ...
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Article
Making the Connection: Ciliary Adhesion Complexes Anchor Basal Bodies to the Actin Cytoskeleton
Antoniades, Ioanna; Stylianou, Panayiota; Skourides, Paris A. (2014)Cilia have been associated with diverse developmental and physiological processes, and defects in cilia underlie a number of genetic conditions. Several lines of evidence support a critical role of the actin cytoskeleton ...
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Book Chapter
Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
Antoniades, Ioanna; Charalambous, Anna; Christodoulou, Neophytos; Zanardelli, Sara; Skourides, Paris A. (Springer, 2019)Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that the role of calpains in cell migration and other ...
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Article
Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins
Skourides, Paris A.; Perera, S. A.; Ren, R. (1999)Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether ...