Browsing by Subject "tyrosine"

Article

Activation of endogenous FAK via expression of its amino terminal domain in xenopus embryos

Petridou, Nicoletta I.; Stylianou, Panayiota; Christodoulou, Neophytos; Rhoads, Daniel S.; Guan, Junlin; Skourides, Paris A. (2012)

Background: The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic ...

Article

Chiral ionic liquids in chromatographic and electrophoretic separations

Kapnissi‐Christodoulou, Constantina P.; Stavrou, Ioannis J.; Mavroudi, Maria C. (2014)

This report provides an overview of the application of chiral ionic liquids (CILs) in separation technology, and particularly in capillary electrophoresis and both gas and liquid chromatography. There is a large number of ...

Article

Computational protein design: The proteus software and selected applications

Simonson, T.; Gaillard, T.; Mignon, D.; Schmidt Am Busch, M.; Lopes, A.; Amara, Najette; Polydorides, Savvas; Sedano, A.; Druart, Karen; Archontis, Georgios Z. (2013)

We describe an automated procedure for protein design, implemented in a flexible software package, called Proteus. System setup and calculation of an energy matrix are done with the XPLOR modeling program and its sophisticated ...

Article

Direct detection of Fe(IV)=O intermediates in the cytochrome aa3 oxidase from Paracoccus denitrificans[H2O2 reaction

Pinakoulaki, Eftychia; Pfitzner, U.; Ludwig, B.; Varotsis, Constantinos (2003)

We report the first evidence for the formation of the "607- and 580-nm forms" in the cytochrome oxidase aa3/H2O2 reaction without the involvement of tyrosine 280. The pKa of the 607-580-nm transition is 7.5. The 607-nm ...

Article

Molecular investigation and long-term clinical progress in Greek Cypriot families with recessive distal renal tubular acidosis and sensorineural deafness due to mutations in the ATP6V1B1 gene

Feldman, M.; Prikis, Marios; Athanasiou, Yiannis; Elia, Avraam; Pierides, Alkis M.; Constantinou-Deltas, Constantinos D. (2006)

The spectrum of distal renal tubular acidosis (dRTA) includes a genetically heterogeneous group of inherited conditions of both autosomal-dominant and recessive mode of inheritance. The basic defect islinked to the renal ...

Article

The phosphoform of the regulatory subunit RII of cyclic AMP-dependent protein kinase possesses intrinsic topoisomerase activity

Constantinou, Andreas I.; Squinto, S. P.; Jungmann, R. A. (1985)

The phosphoform of the type II regulatory subunit (phospho-RII-cAMP) of cAMP-dependent protein kinase from rat liver was found to possess intrinsic topoisomerase activity towards several DNA substrates such as φX174, pBR322, ...

Article

Preparation of a psammaplysene-based library

Georgiades, Savvas N.; Clardy, J. (2006)

(Chemical Equation Presented) A 28-member focused library, based on the pseudosymmetric template of the marine alkaloids psammaplysenes, was prepared from combinations of components that were, in turn, derived from ...

Article

Recognition and discrimination of gases by the oxygen-sensing signal transducer protein HemAT as revealed by FTIR spectroscopy

Pinakoulaki, Eftychia; Yoshimura, H.; Yoshioka, S.; Aono, S.; Varotsis, Constantinos (2006)

The determination of ligand binding properties is a key step in our understanding of gas sensing and discrimination by gas sensory proteins. HemAT is a newly discovered signal transducer heme protein that recognizes O 2 ...

Article

The role of the cross-link His-Tyr in the functional properties of the binuclear center in cytochrome c oxidase

Pinakoulaki, Eftychia; Pfitzner, U.; Ludwig, B.; Varotsis, Constantinos (2002)

Resonance Raman and Fourier transform infrared spectroscopies have been used to study the aa3-type cytochrome c oxidase and the Y280H mutant from Paracoccus denitrificans. The stability of the binuclear center in the absence ...

Article

Total synthesis of psammaplysenes A and B, naturally occurring inhibitors of FOXO1a nuclear export

Georgiades, Savvas N.; Clardy, J. (2005)

(Chemical Equation Presented) Two inhibitors of FOXO1a-mediated nuclear export, psammaplysenes A and B, have been synthesized by a flexible and efficient route. A common starting material, 4-iodophenol, was used to prepare ...

Article

Ultraviolet Absorbance and Circular Dichroism of Pf1 Virus: Nucleotide/Subunit Ratio of Unity, Hyperchromic Tyrosines and DNA Bases, and High Helicity in the Subunits

Kostrikis, Leontios G.; Liu, D. J.; Day, L. A. (1994)

Data have been obtained for the Pfl virion that establish its stoichiometry and conformational features of its DNA and its protein. The absorbance spectrum of the dissociated virus under alkaline denaturing conditions is ...

Article

UV resonance Raman spectroscopy of TTR(105-115): Determination of the pKa of tyrosine

Pieridou, Galatia K.; Hayes, Sophia C. (2009)

The 11-residue peptide fragment from transthyretin (TTR(105-115)) has been investigated using UV resonance Raman spectroscopy. Excitation at 239.5 nm reveals selective enhancement of scattering from two Tyr residues. The ...