Browsing Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences by Author "Ioannou, Androulla"
Now showing items 1-5 of 5
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Article
Comparing intracellular stability and targeting of sulfobetaine quantum dots with other surface chemistries in live cells
Muro, E.; Fragola, A.; Pons, T.; Lequeux, N.; Ioannou, Androulla; Skourides, Paris A.; Dubertret, B. (2012)The in vivo labeling of intracellular components with quantum dots (QDs) is very limited because of QD aggregation in the cell cytoplasm and/or QD confinement into lysosomal compartments. In order to improve intracellular ...
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Article
High-resolution whole-mount in situ hybridization using quantum dot nanocrystals
Ioannou, Androulla; Eleftheriou, Iro; Lubatti, Andrea; Charalambous, Anna; Skourides, Paris A. (2012)The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically ...
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Book Chapter
Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus
Ioannou, Androulla; Eleftheriou, Iro; Charalambous, Anna; Skourides, Paris A. (Springer New York, 2015)The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount ...
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Article
Single-shot optical sectioning using two-color probes in HiLo fluorescence microscopy
Muro, E.; Vermeulen, P.; Ioannou, Androulla; Skourides, Paris A.; Dubertret, B.; Fragola, A.; Loriette, V. (2011)We describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving ...
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Article
Xenopus laevis nucleotide binding protein 1 (xNubp1) is important for convergent extension movements and controls ciliogenesis via regulation of the actin cytoskeleton
Ioannou, Androulla; Santama, Niovi; Skourides, Paris A. (2013)Nucleotide binding protein 1 (Nubp1) is a highly conserved phosphate loop (P-loop) ATPase involved in diverse processes including iron-sulfur protein assembly, centrosome duplication and lung development. Here, we report ...