dc.contributor.author | Gkretsi, V. | en |
dc.contributor.author | Stylianou, A. | en |
dc.contributor.author | Stylianopoulos, T. | en |
dc.creator | Gkretsi, V. | en |
dc.creator | Stylianou, A. | en |
dc.creator | Stylianopoulos, T. | en |
dc.date.accessioned | 2019-05-06T12:23:38Z | |
dc.date.available | 2019-05-06T12:23:38Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/48372 | |
dc.description.abstract | A hallmark of cancer cells is their ability to invade surrounding tissues and form metastases. Cell-extracellular matrix (ECM)-adhesion proteins are crucial in metastasis, connecting tumor ECM with actin cytoskeleton thus enabling cells to respond to mechanical cues. Vasodilator-stimulated phosphoprotein (VASP) is an actin-polymerization regulator which interacts with cell-ECM adhesion protein Migfilin, and regulates cell migration. We compared VASP expression in MCF-7 and MDA-MB-231 breast cancer (BC) cells and found that more invasive MDA-MB-231 cells overexpress VASP. We then utilized a 3-dimensional (3D) approach to study metastasis in MDA-MB-231 cells using a system that considers mechanical forces exerted by the ECM. We prepared 3D collagen I gels of increasing concentration, imaged them by atomic force microscopy, and used them to either embed cells or tumor spheroids, in the presence or absence of VASP. We show, for the first time, that VASP silencing downregulated Migfilin, β-catenin and urokinase plasminogen activator both in 2D and 3D, suggesting a matrix-independent mechanism. Tumor spheroids lacking VASP demonstrated impaired invasion, indicating VASP's involvement in metastasis, which was corroborated by Kaplan-Meier plotter showing high VASP expression to be associated with poor remission-free survival in lymph node-positive BC patients. Hence, VASP may be a novel BC metastasis biomarker. © 2017 Elsevier Inc. | en |
dc.language.iso | eng | en |
dc.source | Experimental cell research | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | breast cancer | en |
dc.subject | Breast Neoplasms | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | protein expression | en |
dc.subject | Cell Adhesion Molecules | en |
dc.subject | unclassified drug | en |
dc.subject | down regulation | en |
dc.subject | protein depletion | en |
dc.subject | Article | en |
dc.subject | metabolism | en |
dc.subject | cytoskeleton protein | en |
dc.subject | human cell | en |
dc.subject | genetics | en |
dc.subject | beta catenin | en |
dc.subject | breast tumor | en |
dc.subject | cell adhesion molecule | en |
dc.subject | Cellular | en |
dc.subject | collagen type 1 | en |
dc.subject | Extracellular matrix | en |
dc.subject | atomic force microscopy | en |
dc.subject | concentration (parameters) | en |
dc.subject | MCF-7 cell line | en |
dc.subject | MDA-MB-231 cell line | en |
dc.subject | tumor spheroid | en |
dc.subject | urokinase | en |
dc.subject | actin binding protein | en |
dc.subject | cancer inhibition | en |
dc.subject | Cytoskeletal Proteins | en |
dc.subject | Down-Regulation | en |
dc.subject | FBLIM1 protein | en |
dc.subject | MCF-7 Cells | en |
dc.subject | Microfilament Proteins | en |
dc.subject | Migfilin | en |
dc.subject | multicellular spheroid | en |
dc.subject | phosphoprotein | en |
dc.subject | Phosphoproteins | en |
dc.subject | Spheroids | en |
dc.subject | Tumor spheroid invasion | en |
dc.subject | Urokinase plasminogen activator | en |
dc.subject | Urokinase-Type Plasminogen Activator | en |
dc.subject | Vasodilator stimulated phosphoprotein | en |
dc.subject | vasodilator-stimulated phosphoprotein | en |
dc.title | Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and urokinase-plasminogen activator (uPA) | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1016/j.yexcr.2017.02.019 | |
dc.description.volume | 352 | |
dc.description.startingpage | 281 | |
dc.description.endingpage | 292 | |
dc.author.faculty | Πολυτεχνική Σχολή / Faculty of Engineering | |
dc.author.department | Τμήμα Μηχανικών Μηχανολογίας και Κατασκευαστικής / Department of Mechanical and Manufacturing Engineering | |
dc.type.uhtype | Article | en |
dc.contributor.orcid | Stylianopoulos, T. [0000-0002-3093-1696] | |
dc.description.totalnumpages | 281-292 | |
dc.gnosis.orcid | 0000-0002-3093-1696 | |