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dc.contributor.authorIoannou, Androullaen
dc.contributor.authorEleftheriou, Iroen
dc.contributor.authorCharalambous, Annaen
dc.contributor.authorSkourides, Paris A.en
dc.creatorIoannou, Androullaen
dc.creatorEleftheriou, Iroen
dc.creatorCharalambous, Annaen
dc.creatorSkourides, Paris A.en
dc.date.accessioned2019-11-04T12:50:45Z
dc.date.available2019-11-04T12:50:45Z
dc.date.issued2015
dc.identifier.isbn978-1-4939-2303-8
dc.identifier.isbn978-1-4939-2302-1
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53138
dc.description.abstractThe photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount fluorescent ISH in Xenopus laevis embryos using far red and near infrared (NIR) quantum dot nanocrystals. Our results demonstrate that QD detection of endogenous messenger RNA is effective even in highly autofluorescent organisms such as Xenopus and therefore holds promise for use in many other model organisms. © Springer Science+Business Media New York 2015. All rights are reserved.en
dc.publisherSpringer New Yorken
dc.sourceIn Situ Hybridization Methodsen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84955420592&doi=10.1007%2f978-1-4939-2303-8_24&partnerID=40&md5=863ebb783937bea0a74bf0d6e0d2af02
dc.subjectNanocrystalsen
dc.subjectQuantum dotsen
dc.subjectXenopusen
dc.subjectFluorescent whole-mount ISHen
dc.subjectMultiplexen
dc.titleSingle and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopusen
dc.typeinfo:eu-repo/semantics/bookChapter
dc.description.startingpage451
dc.description.endingpage462
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeBook Chapteren
dc.contributor.orcidSkourides, Paris A. [0000-0003-3502-5729]


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