dc.contributor.author | Righi, V. | en |
dc.contributor.author | Apidianakis, Yiorgos | en |
dc.contributor.author | Rahme, L. G. | en |
dc.contributor.author | Tzika, A. A. | en |
dc.creator | Righi, V. | en |
dc.creator | Apidianakis, Yiorgos | en |
dc.creator | Rahme, L. G. | en |
dc.creator | Tzika, A. A. | en |
dc.date.accessioned | 2019-11-04T12:52:34Z | |
dc.date.available | 2019-11-04T12:52:34Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 1940-087X | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53338 | |
dc.description.abstract | High-Resolution Magic Angle Spinning (HRMAS) proton magnetic resonance spectroscopy ((1)H-MRS) is a novel non-destructive technique that improves spectral line-widths and allows high-resolution spectra to be obtained from extracts, intact cells, cell cultures, and more importantly intact tissue to investigate relationships between metabolites and cellular processes. In vivo HRMAS (1)H-MRS studies have yet to be reported in the live fruit fly Drosophila melanogaster. Drosophila, as a simpler genetic organism, allows the multiple biological functions and various evolutionarily conserved signaling pathways to be examined at the whole organism level and it is a useful model for investigating genetics and physiology. To this end, we developed and implemented an in vivo HRMAS (1)H-MRS method to investigate live Drosophila at 14.1 T. Here, we outline an HRMAS (1)H-MRS protocol for the molecular characterization of Drosophila with a conventional MR spectrometer equipped with an HRMAS probe. This technique is a novel, in vivo, non-destructive Drosophila metabolite measurement approach, which enables the identification of disease biomarkers and thus may contribute to novel therapeutic development. | en |
dc.source | Journal of visualized experiments : JoVE | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873030467&partnerID=40&md5=b45a966af0fbe543609eb0d13b7bbda9 | |
dc.subject | methodology | en |
dc.subject | article | en |
dc.subject | metabolism | en |
dc.subject | Animals | en |
dc.subject | animal | en |
dc.subject | Magnetic Resonance Spectroscopy | en |
dc.subject | nuclear magnetic resonance spectroscopy | en |
dc.subject | instrumentation | en |
dc.subject | Drosophila melanogaster | en |
dc.title | Magnetic resonance spectroscopy of live Drosophila melanogaster using magic angle spinning. | en |
dc.type | info:eu-repo/semantics/article | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :1</p> | en |
dc.source.abbreviation | J Vis Exp | en |
dc.contributor.orcid | Apidianakis, Yiorgos [0000-0002-7465-3560] | |
dc.gnosis.orcid | 0000-0002-7465-3560 | |