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dc.contributor.authorRighi, V.en
dc.contributor.authorApidianakis, Yiorgosen
dc.contributor.authorRahme, L. G.en
dc.contributor.authorTzika, A. A.en
dc.creatorRighi, V.en
dc.creatorApidianakis, Yiorgosen
dc.creatorRahme, L. G.en
dc.creatorTzika, A. A.en
dc.date.accessioned2019-11-04T12:52:34Z
dc.date.available2019-11-04T12:52:34Z
dc.date.issued2010
dc.identifier.issn1940-087X
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/53338
dc.description.abstractHigh-Resolution Magic Angle Spinning (HRMAS) proton magnetic resonance spectroscopy ((1)H-MRS) is a novel non-destructive technique that improves spectral line-widths and allows high-resolution spectra to be obtained from extracts, intact cells, cell cultures, and more importantly intact tissue to investigate relationships between metabolites and cellular processes. In vivo HRMAS (1)H-MRS studies have yet to be reported in the live fruit fly Drosophila melanogaster. Drosophila, as a simpler genetic organism, allows the multiple biological functions and various evolutionarily conserved signaling pathways to be examined at the whole organism level and it is a useful model for investigating genetics and physiology. To this end, we developed and implemented an in vivo HRMAS (1)H-MRS method to investigate live Drosophila at 14.1 T. Here, we outline an HRMAS (1)H-MRS protocol for the molecular characterization of Drosophila with a conventional MR spectrometer equipped with an HRMAS probe. This technique is a novel, in vivo, non-destructive Drosophila metabolite measurement approach, which enables the identification of disease biomarkers and thus may contribute to novel therapeutic development.en
dc.sourceJournal of visualized experiments : JoVEen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84873030467&partnerID=40&md5=b45a966af0fbe543609eb0d13b7bbda9
dc.subjectmethodologyen
dc.subjectarticleen
dc.subjectmetabolismen
dc.subjectAnimalsen
dc.subjectanimalen
dc.subjectMagnetic Resonance Spectroscopyen
dc.subjectnuclear magnetic resonance spectroscopyen
dc.subjectinstrumentationen
dc.subjectDrosophila melanogasteren
dc.titleMagnetic resonance spectroscopy of live Drosophila melanogaster using magic angle spinning.en
dc.typeinfo:eu-repo/semantics/article
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :1</p>en
dc.source.abbreviationJ Vis Expen
dc.contributor.orcidApidianakis, Yiorgos [0000-0002-7465-3560]
dc.gnosis.orcid0000-0002-7465-3560


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