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Decay of the transient CuB-CO complex is accompanied by formation of the heme Fe-CO complex of cytochrome cbb3-CO at ambient temperature: Evidence from time-resolved fourier transform infrared spectroscopy

dc.contributor.authorStavrakis, Stavrosen
dc.contributor.authorKoutsoupakis, Constantinosen
dc.contributor.authorPinakoulaki, Eftychiaen
dc.contributor.authorUrban, A.en
dc.contributor.authorSaraste, M.en
dc.contributor.authorVarotsis, Constantinosen
dc.creatorStavrakis, Stavrosen
dc.creatorKoutsoupakis, Constantinosen
dc.creatorPinakoulaki, Eftychiaen
dc.creatorUrban, A.en
dc.creatorSaraste, M.en
dc.creatorVarotsis, Constantinosen
dc.date.accessioned2019-11-21T06:22:56Z
dc.date.available2019-11-21T06:22:56Z
dc.date.issued2002
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/56152
dc.description.abstractTime-resolved step-scan Fourier infrared spectroscopy has been used to study the CO-bound cbb3-type cytochrome c oxidase from Pseudomonas stutzeri at room temperature. We observe a single band in the FTIR spectrum at 1956 cm-1 (β-form). The time-resolved data indicate that upon photolysis, CO is transferred from heme b3 (vCO = 1956 cm-1) to CuB (vCO = 2064 cm-1). The decay of the 2065 cm-1 peak (t1/2 = 120 ± 16 ms) and the development of the 1956 cm-1 peak (t1/2 = 144 ± 8 ms ) suggest that formation of the Fe-CO complex is concurrent with the decay of the CuB-CO complex. The intensity ratio of the Fe-CO/CuB-CO (2.15) remains constant for all data points, and thus we conclude that no fraction of CO escapes the binuclear center at 293 K. Copyright © 2002 American Chemical Society.en
dc.sourceJournal of the American Chemical Societyen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0037123251&doi=10.1021%2fja0169825&partnerID=40&md5=5a8eab17f7889e55d269cad947056f1a
dc.subjectarticleen
dc.subjectunclassified drugen
dc.subjectnonhumanen
dc.subjectchemical analysisen
dc.subjectIronen
dc.subjectmolecular interactionen
dc.subjectstructure analysisen
dc.subjectcomplex formationen
dc.subjectinfrared spectroscopyen
dc.subjectenvironmental temperatureen
dc.subjectSpectroscopy, Fourier Transform Infrareden
dc.subjectRaman spectrometryen
dc.subjectcarbon monoxideen
dc.subjectElectron Transport Complex IVen
dc.subjectPseudomonas stutzerien
dc.subjectcopper complexen
dc.subjectheme derivativeen
dc.subjectHemeen
dc.subjectcytochromeen
dc.subjectcytochrome cbb 3en
dc.subjectiron derivativeen
dc.titleDecay of the transient CuB-CO complex is accompanied by formation of the heme Fe-CO complex of cytochrome cbb3-CO at ambient temperature: Evidence from time-resolved fourier transform infrared spectroscopyen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1021/ja0169825
dc.description.volume124
dc.description.issue15
dc.description.startingpage3814
dc.description.endingpage3815
dc.author.faculty002 Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Χημείας / Department of Chemistry
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :50</p>en
dc.source.abbreviationJ.Am.Chem.Soc.en
dc.contributor.orcidPinakoulaki, Eftychia [0000-0003-3320-6112]
dc.contributor.orcidVarotsis, Constantinos [0000-0003-2771-8891]
dc.gnosis.orcid0000-0003-3320-6112
dc.gnosis.orcid0000-0003-2771-8891


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