dc.contributor.author | Stavrakis, Stavros | en |
dc.contributor.author | Pinakoulaki, Eftychia | en |
dc.contributor.author | Urbani, A. | en |
dc.contributor.author | Varotsis, Constantinos | en |
dc.creator | Stavrakis, Stavros | en |
dc.creator | Pinakoulaki, Eftychia | en |
dc.creator | Urbani, A. | en |
dc.creator | Varotsis, Constantinos | en |
dc.date.accessioned | 2019-11-21T06:22:56Z | |
dc.date.available | 2019-11-21T06:22:56Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 1089-5647 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/56153 | |
dc.description.abstract | We report the first vibrational study of NO bound to an oxidized heme-copper oxidase. Cytochrome cbb3 oxidase from P. stutzeri reduces both O2 and NO to H2O and N2O, respectively. The ferric nitrosyl complex of cbb3 exhibits v(N-O) at 1903 cm-1. This frequency is very similar to v(NO) of nitric oxide reductase, the acidic form of Met Mb-NO, but 18 cm-1 lower than that of neutral Met Mb-NO. By monitoring the NO intensity, we estimate that NO dissociates from the heme b3 pocket, without binding to CuB, with k = 1.8 × 10-3 s-1. Therefore, NO binding occurs at the heme site and not at CuB, generating a nitrosonium CuB1+-NO+ species as proposed recently (Torres | en |
dc.description.abstract | J. | en |
dc.description.abstract | Cooper, C.E. | en |
dc.description.abstract | Wilson, M.T. J. Biol. Chem. 1998, 273, 8756-8766). The coordination of NO to cbb3 oxidase and to nitric oxide reductase and Mb is compared and discussed. | en |
dc.source | Journal of Physical Chemistry B | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037137620&doi=10.1021%2fjp026763l&partnerID=40&md5=c99e58abbca9b8c2d2b515413a23770a | |
dc.subject | Positive ions | en |
dc.subject | Temperature | en |
dc.subject | Reduction | en |
dc.subject | Fourier transform infrared spectroscopy | en |
dc.subject | Oxidation | en |
dc.subject | Organometallics | en |
dc.subject | Nitrogen oxides | en |
dc.subject | Molecular vibrations | en |
dc.subject | Dissociation | en |
dc.subject | Chemical bonds | en |
dc.subject | Vibrational studies | en |
dc.title | Fourier transform infrared evidence for a ferric six-coordinate nitrosylheme b3 complex of cytochrome cbb3 oxidase from Pseudomonas stutzeri at ambient temperature | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1021/jp026763l | |
dc.description.volume | 106 | |
dc.description.issue | 50 | |
dc.description.startingpage | 12860 | |
dc.description.endingpage | 12862 | |
dc.author.faculty | 002 Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Χημείας / Department of Chemistry | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :18</p> | en |
dc.source.abbreviation | J Phys Chem B | en |
dc.contributor.orcid | Pinakoulaki, Eftychia [0000-0003-3320-6112] | |
dc.contributor.orcid | Varotsis, Constantinos [0000-0003-2771-8891] | |
dc.gnosis.orcid | 0000-0003-3320-6112 | |
dc.gnosis.orcid | 0000-0003-2771-8891 | |