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Exploring the nano-surface of collagenous and other fibrotic tissues with AFM

dc.contributor.authorStylianou, A.en
dc.contributor.authorGkretsi, V.en
dc.contributor.authorPatrickios, Costas S.en
dc.contributor.authorStylianopoulos, Triantafyllosen
dc.creatorStylianou, A.en
dc.creatorGkretsi, V.en
dc.creatorPatrickios, Costas S.en
dc.creatorStylianopoulos, Triantafyllosen
dc.date.accessioned2019-11-21T06:23:01Z
dc.date.available2019-11-21T06:23:01Z
dc.date.issued2017
dc.identifier.issn1064-3745
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/56172
dc.description.abstractAtomic force microscope (AFM) is a powerful and invaluable tool for imaging and probing the mechanical properties of biological samples at the nanometric scale. The importance of nano-scale characterization and nanomechanics of soft biological tissues is becoming widely appreciated, and AFM offers unique advantages in this direction. In this chapter, we describe the procedure to collect data sets (imaging and mechanical properties measurement) of collagen gels and tumor tissues. We provide step-by-step instructions throughout the procedure, from sample preparation to cantilever calibration, data acquisition, analysis, and visualization, using two commercial AFM systems (PicoPlus and Cypher ES) and software that accompanied the AFM systems and/or are freeware available (WSxM, AtomicJ). Our protocols are written specifically for these two systems and the mentioned softwareen
dc.description.abstracthowever, most of the general concepts can be readily translated to other AFM systems and software. © 2017, Springer Science+Business Media LLC.en
dc.sourceMethods in Molecular Biologyen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85028449646&doi=10.1007%2f978-1-4939-7113-8_29&partnerID=40&md5=e767c458f1c251c6e9f3c1b5dbfe6693
dc.subjectCanceren
dc.subjectElasticityen
dc.subjectStiffnessen
dc.subjectMechanobiologyen
dc.subjectConnective tissuesen
dc.subjectForce mapsen
dc.subjectForce spectroscopyen
dc.subjectYoung’s modulusen
dc.titleExploring the nano-surface of collagenous and other fibrotic tissues with AFMen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1007/978-1-4939-7113-8_29
dc.description.volume1627
dc.description.startingpage453
dc.description.endingpage489
dc.author.faculty002 Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Χημείας / Department of Chemistry
dc.type.uhtypeArticleen
dc.source.abbreviationMethods Mol. Biol.en
dc.contributor.orcidStylianopoulos, Triantafyllos [0000-0002-3093-1696]
dc.contributor.orcidPatrickios, Costas S. [0000-0001-8855-0370]
dc.gnosis.orcid0000-0002-3093-1696
dc.gnosis.orcid0000-0001-8855-0370


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