dc.contributor.author | Tzika, Evangelia D. | en |
dc.contributor.author | Christoforou, Maria | en |
dc.contributor.author | Pispas, Stergios | en |
dc.contributor.author | Zervou, Maria | en |
dc.contributor.author | Papadimitriou, Vassiliki | en |
dc.contributor.author | Sotiroudis, Theodore G. | en |
dc.contributor.author | Leontidis, Epameinondas | en |
dc.contributor.author | Xenakis, Aristotelis | en |
dc.creator | Tzika, Evangelia D. | en |
dc.creator | Christoforou, Maria | en |
dc.creator | Pispas, Stergios | en |
dc.creator | Zervou, Maria | en |
dc.creator | Papadimitriou, Vassiliki | en |
dc.creator | Sotiroudis, Theodore G. | en |
dc.creator | Leontidis, Epameinondas | en |
dc.creator | Xenakis, Aristotelis | en |
dc.date.accessioned | 2019-11-21T06:23:25Z | |
dc.date.available | 2019-11-21T06:23:25Z | |
dc.date.issued | 2011 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/56263 | |
dc.description.abstract | Oxidative enzymatic reactions using horseradish peroxidase (HRP) were carried out in water-in-oil (w/o) microemulsions composed of olive oil/lecithin/1-propanol/water, a model biomimetic system. The substrates used (gallic acid, octyl gallate and 2,2′-azino-bis[3-ethylbenzo-thiazoline-6- sulfonic acid] (ABTS)) have different hydrophobicities and possible locations in the microemulsion system. HRP reactivity with reference to substrate hydrophobicity and structural characteristics of the microemulsions is discussed. The nature of the enzyme microenvironments was examined using dynamic light scattering (DLS), differential scanning calorimetry (DSC) and diffusion NMR (DOSY) methodologies while the location of various enzymatic substrates in the microemulsion phase was assessed by solubility measurements and by taking pressure-area isotherms of mixed monolayers of the substrates with dipalmitoyl-phosphatidylcholine (DPPC), which is a major constituent of lecithin. In contrast to the bulk aqueous phase, in the severely restricted environment of the polar domains of the microemulsion HRP reacted faster with octyl gallate, a substrate that is solubilized at the lipid interfaces. HRP was deactivated in the olive oil microemulsions within a few hours, a phenomenon that has also been observed in other microemulsion systems. © 2011 American Chemical Society. | en |
dc.source | Langmuir | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952588624&doi=10.1021%2fla104848t&partnerID=40&md5=abea8011a4cd689a4a461bfd11a86850 | |
dc.subject | article | en |
dc.subject | metabolism | en |
dc.subject | chemistry | en |
dc.subject | Hydrophobicity | en |
dc.subject | Structural characteristics | en |
dc.subject | Substrates | en |
dc.subject | chemical structure | en |
dc.subject | Microenvironments | en |
dc.subject | nanoparticle | en |
dc.subject | Differential scanning calorimetry | en |
dc.subject | Nanoparticles | en |
dc.subject | nanotechnology | en |
dc.subject | water | en |
dc.subject | Molecular Structure | en |
dc.subject | enzyme activation | en |
dc.subject | Olive oil | en |
dc.subject | Plant Oils | en |
dc.subject | vegetable oil | en |
dc.subject | Phospholipids | en |
dc.subject | enzyme specificity | en |
dc.subject | Substrate Specificity | en |
dc.subject | Monolayers | en |
dc.subject | Aqueous phase | en |
dc.subject | Pressure-area isotherms | en |
dc.subject | Dynamic light scattering | en |
dc.subject | oil | en |
dc.subject | Vegetable oils | en |
dc.subject | Microemulsions | en |
dc.subject | Phase interfaces | en |
dc.subject | Biomimetic systems | en |
dc.subject | Biomimetics | en |
dc.subject | Diffusion NMR | en |
dc.subject | Dipalmitoyl phosphatidylcholine | en |
dc.subject | Enzymatic reaction | en |
dc.subject | Gallic acids | en |
dc.subject | Horseradish peroxidase | en |
dc.subject | Microemulsion systems | en |
dc.subject | Mixed monolayers | en |
dc.subject | Octyl gallate | en |
dc.subject | Oils | en |
dc.subject | Solubility measurement | en |
dc.subject | Sulfonic acid | en |
dc.subject | Thiazoline | en |
dc.subject | Water-in-oil (w/o) microemulsions | en |
dc.subject | Water-in-oil microemulsions | en |
dc.title | Influence of nanoreactor environment and substrate location on the activity of horseradish peroxidase in olive oil based water-in-oil microemulsions | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1021/la104848t | |
dc.description.volume | 27 | |
dc.description.issue | 6 | |
dc.description.startingpage | 2692 | |
dc.description.endingpage | 2700 | |
dc.author.faculty | 002 Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Χημείας / Department of Chemistry | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :9</p> | en |
dc.source.abbreviation | Langmuir | en |
dc.contributor.orcid | Leontidis, Epameinondas [0000-0003-4427-0398] | |
dc.gnosis.orcid | 0000-0003-4427-0398 | |