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Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
(2011)
Background: Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within ...
In vivo, site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing
(2012)
The ability to target proteins with nanostructures and/or nanodevices in vivo is important for understanding and controlling their biological function. Quantum dots (QDs) serve as an ideal model nanostructure due to their ...
Making the Connection: Ciliary Adhesion Complexes Anchor Basal Bodies to the Actin Cytoskeleton
(2014)
Cilia have been associated with diverse developmental and physiological processes, and defects in cilia underlie a number of genetic conditions. Several lines of evidence support a critical role of the actin cytoskeleton ...
Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells
(2017)
We previously identified focal adhesion kinase (FAK) as an important regulator of ciliogenesis in multiciliated cells. FAK and other focal adhesion (FA) proteins associate with the basal bodies and their striated rootlets ...
1,2,3-Dithiazoles-new reversible melanin synthesis inhibitors: A chemical genomics study
(2015)
A chemical genomic screen of an in-house library of small molecule heterocycles was carried out using Xenopus laevis embryos. This led to the identification of N-(4-chloro-5H-1,2,3-dithiazol-5-ylidene)-4-methoxyaniline ...
Determining Temporal and Spatial Expression of Calpains in Amphibians
(Springer, 2019)
Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that Calpains have been identified in the Xenopus genome, ...
Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
(Springer, 2019)
Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that the role of calpains in cell migration and other ...