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dc.contributor.authorStoyianni, A.en
dc.contributor.authorGoussia, Annaen
dc.contributor.authorPentheroudakis, Georgeen
dc.contributor.authorSiozopoulou, V.en
dc.contributor.authorIoachim, E.en
dc.contributor.authorKrikelis, D.en
dc.contributor.authorGolfinopoulos, Vassilisen
dc.contributor.authorCervantes, A.en
dc.contributor.authorBobos, M.en
dc.contributor.authorFotsis, T.en
dc.contributor.authorBellou, S.en
dc.contributor.authorFountzilas, Georgeen
dc.contributor.authorMalamou-Mitsi, Vassiliki D.en
dc.contributor.authorPavlidis, Nicholasen
dc.creatorStoyianni, A.en
dc.creatorGoussia, Annaen
dc.creatorPentheroudakis, Georgeen
dc.creatorSiozopoulou, V.en
dc.creatorIoachim, E.en
dc.creatorKrikelis, D.en
dc.creatorGolfinopoulos, Vassilisen
dc.creatorCervantes, A.en
dc.creatorBobos, M.en
dc.creatorFotsis, T.en
dc.creatorBellou, S.en
dc.creatorFountzilas, Georgeen
dc.creatorMalamou-Mitsi, Vassiliki D.en
dc.creatorPavlidis, Nicholasen
dc.date.accessioned2018-06-22T09:53:20Z
dc.date.available2018-06-22T09:53:20Z
dc.date.issued2012
dc.identifier.urihttps://gnosis.library.ucy.ac.cy/handle/7/41784
dc.description.abstractBackground: The epithelial to mesenchymal transition (EMT) has been associated with metastatic dissemination and poor outcome in several solid tumour types. Our aim was to study its incidence and its prognostic significance in cancer of unknown primary (CUP). Patients and Methods: One hundred tumour samples of CUP were loaded in tissue microarrays and were studied for immunohistochemical (IHC) expression of E-cadherin, N-cadherin, vimentin, the EMT transcription factor (SNAIL) and the stem cell marker octamer-binding transcription marker 4(OCT4). An EMT phenotype was defined as low expression of E-cadherin, expression of N-cadherin with/without vimentin with concomitant expression of SNAIL, as assessed by percentage of tumour cell staining. Results: Among 100 CUP cases, the histological diagnosis was adenocarcinoma in 55, squamous carcinoma in 20 and undifferentiated carcinoma in 15, with a high grade seen in 46. Therapy consisted of palliative chemotherapy, mostly platinum based. The median progression-free survival and overall survival (OS) were 7 and 12 months respectively. Distributional studies resulted in selection of IHC cut-offs for E-cadherin (negative when expressed in <60% of tumour cells), N-cadherin, vimentin (positive when expressed in ≥40% of tumour cells), SNAIL (positive when stained in ≥80% of tumour cells). An EMT phenotype was observed in 8 cases (8.1%) and was strongly associated with poor OS (median OS EMT -=13 months vs. median OS EMT +=8 months, p=0.023). When we used staining intensity (H-Score), an EMT phenotype was observed in 16 patients and carried borderline adverse prognostic utility for outcome (median OS 9 vs. 14 months, p=0.07). The presence of the EMT phenotype correlated significantly with male gender, high grade and presence of visceral metastases (χ 2 p<0.05), while EMT mediator expression was correlated to high NOTCH 2/3 expression. Other factors, prognostic for poor survival, were male gender, PS≥2, non-platinum therapy (χ 2 p<0.05). Conclusion: EMT is infrequently seen in tumours of CUP. However, an adverse prognostic significance for patient outcome has been identified and may warrant studies of therapeutic targeting.en
dc.language.isoengen
dc.sourceAnticancer Researchen
dc.subjectArticleen
dc.subjectHumanen
dc.subjectNeoplasmsen
dc.subject80 and overen
dc.subjectAgeden
dc.subjectHumansen
dc.subjectAdulten
dc.subjectControlled studyen
dc.subjectFemaleen
dc.subjectMajor clinical studyen
dc.subjectMiddle ageden
dc.subjectCancer survivalen
dc.subjectPriority journalen
dc.subjectHuman tissueen
dc.subjectPlatinumen
dc.subjectPrognosisen
dc.subjectOverall survivalen
dc.subjectCancer prognosisen
dc.subjectMaleen
dc.subjectCorrelation analysisen
dc.subjectProtein expressionen
dc.subjectImmunohistochemistryen
dc.subjectCancer gradingen
dc.subjectTumor cellen
dc.subjectCancer incidenceen
dc.subjectDisease associationen
dc.subjectIncidenceen
dc.subjectAdenocarcinomaen
dc.subjectSquamous cell carcinomaen
dc.subjectCancer of unknown primary siteen
dc.subjectUnknown primaryen
dc.subjectVisceral metastasisen
dc.subjectUndifferentiated carcinomaen
dc.subjectPhenotypeen
dc.subjectHuman cellen
dc.subjectCancer of unknown primaryen
dc.subjectProgression free survivalen
dc.subjectTissue array analysisen
dc.subjectTissue microarrayen
dc.subjectTranscription factorsen
dc.subjectStainingen
dc.subjectNerve cell adhesion moleculeen
dc.subjectTranscription factor snailen
dc.subjectUvomorulinen
dc.subjectCadherinsen
dc.subjectE-cadherinen
dc.subjectEpithelial mesenchymal transitionen
dc.subjectEpithelial-mesenchymal transitionen
dc.subjectN-cadherinen
dc.subjectNotch1en
dc.subjectOct4en
dc.subjectOctamer transcription factor-3en
dc.subjectSnailen
dc.subjectVimentinen
dc.titleImmunohistochemical study of the epithelial-mesenchymal transition phenotype in cancer of unknown primary: Incidence, correlations and prognostic utilityen
dc.typeinfo:eu-repo/semantics/article
dc.description.volume32
dc.description.issue4
dc.description.startingpage1273
dc.description.endingpage1281
dc.author.facultyΙατρική Σχολή / Medical School
dc.type.uhtypeArticleen


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