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dc.contributor.authorBurdette, J. E.en
dc.contributor.authorChen, S. -Nen
dc.contributor.authorLu, Z. -Zen
dc.contributor.authorXu, H.en
dc.contributor.authorWhite, B. E. P.en
dc.contributor.authorFabricant, D. S.en
dc.contributor.authorLiu, J.en
dc.contributor.authorFong, H. H. S.en
dc.contributor.authorFarnsworth, N. R.en
dc.contributor.authorConstantinou, Andreas I.en
dc.contributor.authorVan Breemen, R. B.en
dc.contributor.authorPezzuto, J. M.en
dc.contributor.authorBolton, J. L.en
dc.creatorBurdette, J. E.en
dc.creatorChen, S. -Nen
dc.creatorLu, Z. -Zen
dc.creatorXu, H.en
dc.creatorWhite, B. E. P.en
dc.creatorFabricant, D. S.en
dc.creatorLiu, J.en
dc.creatorFong, H. H. S.en
dc.creatorFarnsworth, N. R.en
dc.creatorConstantinou, Andreas I.en
dc.creatorVan Breemen, R. B.en
dc.creatorPezzuto, J. M.en
dc.creatorBolton, J. L.en
dc.date.accessioned2019-11-04T12:50:17Z
dc.date.available2019-11-04T12:50:17Z
dc.date.issued2002
dc.identifier.urihttp://gnosis.library.ucy.ac.cy/handle/7/52958
dc.description.abstractThe roots/rhizomes of Cimicifuga racemosa L. (Nutt.) (black cohosh) have traditionally been used to treat menopausal symptoms through an unknown mechanism of action. In an effort to determine if black cohosh had additional health benefits, methanol extracts were investigated for their potential to scavenge reactive oxygen species and to protect against menadione-induced DNA damage. These extracts effectively scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. In addition, the extracts showed dose-dependent decreases in DNA single-strand breaks and oxidized bases induced by the quinone menadione using the comet (single-cell gel electrophoresis assay) and fragment length associated repair enzyme assays, respectively. Bioassay-directed fractionation of the methanolic extracts using the DPPH assay as a monitor led to the isolation of nine antioxidant active compounds: caffeic acid (1), methyl caffeate (2), ferulic acid (3), isoferulic acid (4), fukinolic acid (5), cimicifugic acid A (6), cimicifugic acid B (7), cimicifugic acid F (8), cimiracemate A (9), and cimiracemate B (10). Six of these antioxidants were found to reduce menadione-induced DNA damage in cultured S30 breast cancer cells with the following order of potency: methyl caffeate (2) > caffeic acid (1) > ferulic acid (3) > cimiracemate A (9) > cimiracemate B (10) > fukinolic acid (5). These data suggest that black cohosh can protect against cellular DNA damage caused by reactive oxygen species by acting as antioxidants.en
dc.sourceJournal of Agricultural and Food Chemistryen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0037145929&doi=10.1021%2fjf020725h&partnerID=40&md5=ce19d42f14e53b5b6fb993fc685af655
dc.subjectarticleen
dc.subjecthumanen
dc.subjectHumansen
dc.subjectbreast canceren
dc.subjectBreast Neoplasmsen
dc.subjectdrug effecten
dc.subjectunclassified drugen
dc.subjectestrogen receptoren
dc.subjectoxidative stressen
dc.subjectreactive oxygen metaboliteen
dc.subjecthuman cellen
dc.subjectgene expressionen
dc.subjectgeneticsen
dc.subjectDNAen
dc.subjectmenopauseen
dc.subjectchemistryen
dc.subjectplant extracten
dc.subjectgel electrophoresisen
dc.subjectbreast tumoren
dc.subjectcancer cell cultureen
dc.subjectisolation and purificationen
dc.subjectestrogen receptor alphaen
dc.subjectAntioxidantsen
dc.subjectnuclear magnetic resonance spectroscopyen
dc.subjectoxidation reduction reactionen
dc.subjectbioassayen
dc.subjectcell cultureen
dc.subjectgenetic transfectionen
dc.subjectDNA damageen
dc.subjectReactive oxygen speciesen
dc.subject1,1 diphenyl 2 picrylhydrazylen
dc.subject2,2-diphenyl-1-picrylhydrazylen
dc.subjectAntioxidanten
dc.subjectantioxidant activityen
dc.subjectcaffeic acid derivativeen
dc.subjectCaulophyllumen
dc.subjectcimicifugaen
dc.subjectCimicifuga dahuricaen
dc.subjectCimicifuga racemosaen
dc.subjectCimicifuga racemosa (black cohosh, Actaea racemosa)en
dc.subjectCimicifuga racemosa extracten
dc.subjectcomet assayen
dc.subjectDNA strand breakageen
dc.subjectenzyme assayen
dc.subjectferulic aciden
dc.subjectFree Radical Scavengersen
dc.subjectfukinolic aciden
dc.subjectgallic aciden
dc.subjectMenadioneen
dc.subjectmethanolen
dc.subjectphytochemistryen
dc.subjectPicratesen
dc.subjectpicric aciden
dc.subjectPlant Extractsen
dc.subjectplant rooten
dc.subjectReceptors, Estrogenen
dc.subjectscavengeren
dc.subjectSingle-cell gel electrophoresis assay (comet assay)en
dc.subjectthin layer chromatographyen
dc.subjecttraditional medicineen
dc.subjectTransfectionen
dc.subjectTumor Cells, Cultureden
dc.subjectVitamin K 3en
dc.titleBlack cohosh (Cimicifuga racemosa L.) protects against menadione-induced DNA damage through scavenging of reactive oxygen species: Bioassay-directed isolation and characterization of active principlesen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1021/jf020725h
dc.description.volume50
dc.description.startingpage7022
dc.description.endingpage7028
dc.author.facultyΣχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences
dc.author.departmentΤμήμα Βιολογικών Επιστημών / Department of Biological Sciences
dc.type.uhtypeArticleen
dc.description.notes<p>Cited By :67</p>en
dc.source.abbreviationJ.Agric.Food Chem.en
dc.contributor.orcidConstantinou, Andreas I. [0000-0003-0365-1821]
dc.gnosis.orcid0000-0003-0365-1821


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