dc.contributor.author | Burdette, J. E. | en |
dc.contributor.author | Chen, S. -N | en |
dc.contributor.author | Lu, Z. -Z | en |
dc.contributor.author | Xu, H. | en |
dc.contributor.author | White, B. E. P. | en |
dc.contributor.author | Fabricant, D. S. | en |
dc.contributor.author | Liu, J. | en |
dc.contributor.author | Fong, H. H. S. | en |
dc.contributor.author | Farnsworth, N. R. | en |
dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Van Breemen, R. B. | en |
dc.contributor.author | Pezzuto, J. M. | en |
dc.contributor.author | Bolton, J. L. | en |
dc.creator | Burdette, J. E. | en |
dc.creator | Chen, S. -N | en |
dc.creator | Lu, Z. -Z | en |
dc.creator | Xu, H. | en |
dc.creator | White, B. E. P. | en |
dc.creator | Fabricant, D. S. | en |
dc.creator | Liu, J. | en |
dc.creator | Fong, H. H. S. | en |
dc.creator | Farnsworth, N. R. | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Van Breemen, R. B. | en |
dc.creator | Pezzuto, J. M. | en |
dc.creator | Bolton, J. L. | en |
dc.date.accessioned | 2019-11-04T12:50:17Z | |
dc.date.available | 2019-11-04T12:50:17Z | |
dc.date.issued | 2002 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/52958 | |
dc.description.abstract | The roots/rhizomes of Cimicifuga racemosa L. (Nutt.) (black cohosh) have traditionally been used to treat menopausal symptoms through an unknown mechanism of action. In an effort to determine if black cohosh had additional health benefits, methanol extracts were investigated for their potential to scavenge reactive oxygen species and to protect against menadione-induced DNA damage. These extracts effectively scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. In addition, the extracts showed dose-dependent decreases in DNA single-strand breaks and oxidized bases induced by the quinone menadione using the comet (single-cell gel electrophoresis assay) and fragment length associated repair enzyme assays, respectively. Bioassay-directed fractionation of the methanolic extracts using the DPPH assay as a monitor led to the isolation of nine antioxidant active compounds: caffeic acid (1), methyl caffeate (2), ferulic acid (3), isoferulic acid (4), fukinolic acid (5), cimicifugic acid A (6), cimicifugic acid B (7), cimicifugic acid F (8), cimiracemate A (9), and cimiracemate B (10). Six of these antioxidants were found to reduce menadione-induced DNA damage in cultured S30 breast cancer cells with the following order of potency: methyl caffeate (2) > caffeic acid (1) > ferulic acid (3) > cimiracemate A (9) > cimiracemate B (10) > fukinolic acid (5). These data suggest that black cohosh can protect against cellular DNA damage caused by reactive oxygen species by acting as antioxidants. | en |
dc.source | Journal of Agricultural and Food Chemistry | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037145929&doi=10.1021%2fjf020725h&partnerID=40&md5=ce19d42f14e53b5b6fb993fc685af655 | |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | breast cancer | en |
dc.subject | Breast Neoplasms | en |
dc.subject | drug effect | en |
dc.subject | unclassified drug | en |
dc.subject | estrogen receptor | en |
dc.subject | oxidative stress | en |
dc.subject | reactive oxygen metabolite | en |
dc.subject | human cell | en |
dc.subject | gene expression | en |
dc.subject | genetics | en |
dc.subject | DNA | en |
dc.subject | menopause | en |
dc.subject | chemistry | en |
dc.subject | plant extract | en |
dc.subject | gel electrophoresis | en |
dc.subject | breast tumor | en |
dc.subject | cancer cell culture | en |
dc.subject | isolation and purification | en |
dc.subject | estrogen receptor alpha | en |
dc.subject | Antioxidants | en |
dc.subject | nuclear magnetic resonance spectroscopy | en |
dc.subject | oxidation reduction reaction | en |
dc.subject | bioassay | en |
dc.subject | cell culture | en |
dc.subject | genetic transfection | en |
dc.subject | DNA damage | en |
dc.subject | Reactive oxygen species | en |
dc.subject | 1,1 diphenyl 2 picrylhydrazyl | en |
dc.subject | 2,2-diphenyl-1-picrylhydrazyl | en |
dc.subject | Antioxidant | en |
dc.subject | antioxidant activity | en |
dc.subject | caffeic acid derivative | en |
dc.subject | Caulophyllum | en |
dc.subject | cimicifuga | en |
dc.subject | Cimicifuga dahurica | en |
dc.subject | Cimicifuga racemosa | en |
dc.subject | Cimicifuga racemosa (black cohosh, Actaea racemosa) | en |
dc.subject | Cimicifuga racemosa extract | en |
dc.subject | comet assay | en |
dc.subject | DNA strand breakage | en |
dc.subject | enzyme assay | en |
dc.subject | ferulic acid | en |
dc.subject | Free Radical Scavengers | en |
dc.subject | fukinolic acid | en |
dc.subject | gallic acid | en |
dc.subject | Menadione | en |
dc.subject | methanol | en |
dc.subject | phytochemistry | en |
dc.subject | Picrates | en |
dc.subject | picric acid | en |
dc.subject | Plant Extracts | en |
dc.subject | plant root | en |
dc.subject | Receptors, Estrogen | en |
dc.subject | scavenger | en |
dc.subject | Single-cell gel electrophoresis assay (comet assay) | en |
dc.subject | thin layer chromatography | en |
dc.subject | traditional medicine | en |
dc.subject | Transfection | en |
dc.subject | Tumor Cells, Cultured | en |
dc.subject | Vitamin K 3 | en |
dc.title | Black cohosh (Cimicifuga racemosa L.) protects against menadione-induced DNA damage through scavenging of reactive oxygen species: Bioassay-directed isolation and characterization of active principles | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1021/jf020725h | |
dc.description.volume | 50 | |
dc.description.startingpage | 7022 | |
dc.description.endingpage | 7028 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :67</p> | en |
dc.source.abbreviation | J.Agric.Food Chem. | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 | |