dc.contributor.author | Cretu, E. | en |
dc.contributor.author | Karonen, M. | en |
dc.contributor.author | Salminen, J. -P | en |
dc.contributor.author | Mircea, C. | en |
dc.contributor.author | Trifan, A. | en |
dc.contributor.author | Charalambous, Christina | en |
dc.contributor.author | Constantinou, Andreas I. | en |
dc.contributor.author | Miron, A. | en |
dc.creator | Cretu, E. | en |
dc.creator | Karonen, M. | en |
dc.creator | Salminen, J. -P | en |
dc.creator | Mircea, C. | en |
dc.creator | Trifan, A. | en |
dc.creator | Charalambous, Christina | en |
dc.creator | Constantinou, Andreas I. | en |
dc.creator | Miron, A. | en |
dc.date.accessioned | 2019-11-04T12:50:29Z | |
dc.date.available | 2019-11-04T12:50:29Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1096-620X | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53038 | |
dc.description.abstract | A crude hydromethanolic extract from Pinus brutia bark and its fractions (diethyl ether, ethyl acetate, n-butanol, and aqueous fractions) were studied with regard to their phenolic content and antioxidant activities. The total phenolics and proanthocyanidins in each extract were quantified by spectrophotometric methods | en |
dc.description.abstract | the polyphenolic profile was analyzed by RP-HPLC-DAD-ESI-MS. All extracts were tested with regard to their ability to scavenge free radicals (ABTS radical cation, superoxide and hydroxyl radicals), reduce ferric ions, and inhibit 15-lipoxygenase. P. brutia bark extracts had high phenolic contents (303.79±7.34-448.90±1.39 mg/g). Except diethyl ether extract, all other extracts contained proanthocyanidins ranging from 225.79±3.94 to 250.40±1.44 mg/g. Several polyphenols were identified by RP-HPLC-DAD-ESI-MS: taxifolin in diethyl ether extract, a taxifolin-O-hexoside, catechin, procyanidin dimers, and trimers in ethyl acetate extract. Except diethyl ether extract, all other extracts were effective scavengers of superoxide and hydroxyl radicals (EC50=33.5±1.1-54. 93±2.85 μg/mL and 0.47±0.06-0.6±0.0 mg/mL, respectively). All extracts had noticeable 15-lipoxygenase inhibitory effects (EC50=22.47±0.75-34.43±2.25 μg/mL). We conclude that P. brutia bark is very promising for the dietary supplements industry due to its high free radical scavenging and 15-lipoxygenase inhibitory effects. © 2013 Mary Ann Liebert, Inc., and Korean Society of Food Science and Nutrition. | en |
dc.source | Journal of Medicinal Food | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887902409&doi=10.1089%2fjmf.2013.0050&partnerID=40&md5=ec8917bbb40802dff7f8c40aedae2dd9 | |
dc.subject | article | en |
dc.subject | controlled study | en |
dc.subject | priority journal | en |
dc.subject | unclassified drug | en |
dc.subject | diet supplementation | en |
dc.subject | Enzyme Inhibitors | en |
dc.subject | in vitro study | en |
dc.subject | plant extract | en |
dc.subject | Antioxidants | en |
dc.subject | ferric ion | en |
dc.subject | ultraviolet spectroscopy | en |
dc.subject | Free Radicals | en |
dc.subject | Hydroxyl radical | en |
dc.subject | antioxidant activity | en |
dc.subject | Plant Extracts | en |
dc.subject | quercetin | en |
dc.subject | catechin | en |
dc.subject | antioxidant | en |
dc.subject | 15-lipoxygenase | en |
dc.subject | arachidonate 15 lipoxygenase | en |
dc.subject | Arachidonate 15-Lipoxygenase | en |
dc.subject | bark | en |
dc.subject | Calabrian pine | en |
dc.subject | electrospray mass spectrometry | en |
dc.subject | fractionation | en |
dc.subject | pine | en |
dc.subject | Pinus | en |
dc.subject | Pinus brutia | en |
dc.subject | Pinus brutia extract | en |
dc.subject | Plant Bark | en |
dc.subject | polyphenol | en |
dc.subject | Polyphenols | en |
dc.subject | proanthocyanidin derivative | en |
dc.subject | Proanthocyanidins | en |
dc.subject | procyanidin | en |
dc.subject | reversed phase high performance liquid chromatography | en |
dc.subject | RP-HPLC-DAD-ESI-MS | en |
dc.subject | superoxide | en |
dc.subject | Superoxide anion radical | en |
dc.subject | Taxifolin | en |
dc.title | In vitro study on the antioxidant activity of a polyphenol-rich extract from pinus brutia bark and its fractions | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1089/jmf.2013.0050 | |
dc.description.volume | 16 | |
dc.description.startingpage | 984 | |
dc.description.endingpage | 991 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :7</p> | en |
dc.source.abbreviation | J.Med.Food | en |
dc.contributor.orcid | Constantinou, Andreas I. [0000-0003-0365-1821] | |
dc.gnosis.orcid | 0000-0003-0365-1821 | |