dc.contributor.author | Hadjinicolaou, Andreas V. | en |
dc.contributor.author | Farcas, Gabriella A. | en |
dc.contributor.author | Demetriou, Victoria L. | en |
dc.contributor.author | Mazzulli, T. | en |
dc.contributor.author | Poutanen, Susan M. | en |
dc.contributor.author | Willey, B. M. | en |
dc.contributor.author | Low, D. E. | en |
dc.contributor.author | Butany, Jagdish W. | en |
dc.contributor.author | Asa, S. L. | en |
dc.contributor.author | Kain, K. C. | en |
dc.contributor.author | Kostrikis, Leontios G. | en |
dc.creator | Hadjinicolaou, Andreas V. | en |
dc.creator | Farcas, Gabriella A. | en |
dc.creator | Demetriou, Victoria L. | en |
dc.creator | Mazzulli, T. | en |
dc.creator | Poutanen, Susan M. | en |
dc.creator | Willey, B. M. | en |
dc.creator | Low, D. E. | en |
dc.creator | Butany, Jagdish W. | en |
dc.creator | Asa, S. L. | en |
dc.creator | Kain, K. C. | en |
dc.creator | Kostrikis, Leontios G. | en |
dc.date.accessioned | 2019-11-04T12:50:41Z | |
dc.date.available | 2019-11-04T12:50:41Z | |
dc.date.issued | 2011 | |
dc.identifier.uri | http://gnosis.library.ucy.ac.cy/handle/7/53120 | |
dc.description.abstract | Emerging infectious diseases have caused a global effort for development of fast and accurate detection techniques. The rapidly mutating nature of viruses presents a major difficulty, highlighting the need for specific detection of genetically diverse strains. One such infectious agent is SARS-associated coronavirus (SARS-CoV), which emerged in 2003. This study aimed to develop a real-time RT-PCR detection assay specific for SARS-CoV, taking into account its intrinsic polymorphic nature due to genetic drift and recombination and the possibility of continuous and multiple introductions of genetically non-identical strains into the human population, by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV S, E, M and N genes. These were applied in simple, reproducible duplex and multiplex real-time PCR assays on 25 post-mortem samples and constructed RNA controls, and they demonstrated high target detection ability and specificity. This assay can readily be adapted for detection of other emerging and rapidly mutating pathogens. © 2011 Springer-Verlag. | en |
dc.source | Archives of Virology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953219732&doi=10.1007%2fs00705-010-0906-7&partnerID=40&md5=b716b9da8eecf1d8e737d0e3cfd1c042 | |
dc.subject | methodology | en |
dc.subject | article | en |
dc.subject | human | en |
dc.subject | Humans | en |
dc.subject | evaluation | en |
dc.subject | severe acute respiratory syndrome | en |
dc.subject | genetics | en |
dc.subject | reverse transcription polymerase chain reaction | en |
dc.subject | virology | en |
dc.subject | sensitivity and specificity | en |
dc.subject | chemistry | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | molecular diagnosis | en |
dc.subject | Molecular Diagnostic Techniques | en |
dc.subject | mutation | en |
dc.subject | isolation and purification | en |
dc.subject | Coronavirus | en |
dc.subject | oligonucleotide probe | en |
dc.subject | Oligonucleotide Probes | en |
dc.subject | SARS coronavirus | en |
dc.subject | SARS Virus | en |
dc.title | Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): A general methodology for detecting rapidly mutating viruses | en |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.1007/s00705-010-0906-7 | |
dc.description.volume | 156 | |
dc.description.startingpage | 671 | |
dc.description.endingpage | 680 | |
dc.author.faculty | Σχολή Θετικών και Εφαρμοσμένων Επιστημών / Faculty of Pure and Applied Sciences | |
dc.author.department | Τμήμα Βιολογικών Επιστημών / Department of Biological Sciences | |
dc.type.uhtype | Article | en |
dc.description.notes | <p>Cited By :5</p> | en |
dc.source.abbreviation | Arch.Virol. | en |
dc.contributor.orcid | Kostrikis, Leontios G. [0000-0002-5340-7109] | |
dc.gnosis.orcid | 0000-0002-5340-7109 | |